Pou2f2 (NM_001163556) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR224033
Pou2f2 (Myc-DDK-tagged) - Mouse POU domain, class 2, transcription factor 2 (Pou2f2), transcript variant 1
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pou2f2
Synonyms Oct-2; Oct2a; Oct2b; Oct2c; Oct2d; Otf-2; Otf2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR224033 representing NM_001163556
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCATTCCAGCATGGGGGCTCCAGAAATAAGAATGTCTAAGCCCCTGGAGGCCGAGAAGCAAAGTC
TGGACTCCCCGTCAGAGCACACAGACACCGAAAGAAATGGACCCGACATTAACCATCAGAACCCCCAGAA
TAAAGCGTCCCCATTCTCTGTGTCCCCAACTGGCCCCAGCACCAAGATCAAGGCTGAAGACCCCAGTGGC
GATTCAGCCCCAGCAGCACCCCCGCCCCCCCAGCCGGCTCAGCCTCATCTGCCCCAGGCCCAACTCATGC
TGACGGGCAGCCAGCTAGCTGGGGACATACAGCAACTCCTCCAGCTCCAGCAGCTGGTGCTTGTCCCCGG
CCACCACCTCCAGCCACCTGCTCAGTTCCTGCTGCCACAGGCACAGCAGAGTCAGCCAGGCCTGCTACCA
ACGCCAAATCTATTCCAGCTACCTCAACAAACCCAGGGAGCTCTCCTGACCTCCCAGCCCCGGGCTGGGC
TTCCTACACAGCCCCCGAAATGCTTGGAGCCGCCCTCCCACCCGGAGGAGCCCAGCGATCTGGAGGAGCT
GGAACAGTTTGCTCGCACCTTCAAGCAACGCCGCATCAAGCTGGGCTTCACACAGGGTGATGTGGGCCTG
GCCATGGGCAAGCTCTATGGCAACGACTTCAGCCAAACGACCATTTCCCGCTTCGAGGCCCTCAACCTGA
GCTTCAAGAACATGTGTAAACTCAAGCCCCTCCTGGAGAAGTGGCTCAACGACGCAGAGACTATGTCTGT
GGATTCAAGCCTACCCAGCCCAAACCAGCTGAGCAGCCCCAGCCTGGGTTTCGACGGGCTGCCGGGGCGG
AGACGCAAGAAGAGGACCAGCATCGAGACGAATGTCCGCTTCGCCTTAGAGAAGAGTTTCCTAGCGAACC
AGAAGCCTACCTCAGAGGAGATCCTGCTGATCGCAGAGCAGCTGCACATGGAGAAGGAAGTGATCCGCGT
CTGGTTCTGCAACCGGCGCCAGAAGGAGAAACGCATCAACCCTTGCAGTGCGGCCCCCATGCTGCCCAGC
CCGGGAAAGCCGACCAGCTACAGCCCTCACCTGGTCACACCCCAAGGGGGCGCAGGGACCTTACCATTGT
CCCAAGCTTCTAGCAGTCTGAGCACAACAGTTACTACCTTATCCTCAGCTGTGGGGACGCTCCATCCCAG
CCGGACAGCAGGAGGGGGTGGGGGTGGGGGCGGAGCTGCGCCCCCCCTCAATTCCATCCCCTCTGTCACT
CCCCCACCCCCGGCCACCACCAACAGCACAAACCCGAGCCCTCAAGGCAGCCACTCGGCTATTGGCTTGT
CGGGCCTGAACCCCAGCGCGGGCCCTGGCCTCTGGTGGAACCCTGCCCCTTACCAGCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR224033 representing NM_001163556
Red=Cloning site Green=Tags(s)

MVHSSMGAPEIRMSKPLEAEKQSLDSPSEHTDTERNGPDINHQNPQNKASPFSVSPTGPSTKIKAEDPSG
DSAPAAPPPPQPAQPHLPQAQLMLTGSQLAGDIQQLLQLQQLVLVPGHHLQPPAQFLLPQAQQSQPGLLP
TPNLFQLPQQTQGALLTSQPRAGLPTQPPKCLEPPSHPEEPSDLEELEQFARTFKQRRIKLGFTQGDVGL
AMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAETMSVDSSLPSPNQLSSPSLGFDGLPGR
RRKKRTSIETNVRFALEKSFLANQKPTSEEILLIAEQLHMEKEVIRVWFCNRRQKEKRINPCSAAPMLPS
PGKPTSYSPHLVTPQGGAGTLPLSQASSSLSTTVTTLSSAVGTLHPSRTAGGGGGGGGAAPPLNSIPSVT
PPPPATTNSTNPSPQGSHSAIGLSGLNPSAGPGLWWNPAPYQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163556
ORF Size 1389 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163556.1, NP_001157028.1
RefSeq Size 3249 bp
RefSeq ORF 1392 bp
Locus ID 18987
UniProt ID Q00196
Cytogenetics 7 13.73 cM
MW 49.9 kDa
Summary Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform OCT2.5 activates the U2 small nuclear RNA (snRNA) promoter. Isoforms OCT2.1, OCT2.2 and OCT2.3 activate octamer-containing promoters. Isoforms OCT2.4 and OCT2.5 repress some promoters and activate others. Isoform OCT2.7 is unable to bind to the octamer motif, but can still activate the beta-casein gene promoter at low levels.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001163556" in other vectors (4)
SKU Description Size Price
MC216281 Pou2f2 (untagged) - Mouse POU domain, class 2, transcription factor 2 (Pou2f2), transcript variant 1, (10ug) 10 ug
$457.00
MG224033 Pou2f2 (tGFP-tagged) - Mouse POU domain class 2 transcription factor 2 (Pou2f2) transcript variant 1, (10ug) 10 ug
$703.00
MR224033L3 Lenti ORF clone of Pou2f2 (Myc-DDK-tagged) - Mouse POU domain, class 2, transcription factor 2 (Pou2f2), transcript variant 1 10 ug
$803.00
MR224033L4 Lenti ORF clone of Pou2f2 (mGFP-tagged) - Mouse POU domain, class 2, transcription factor 2 (Pou2f2), transcript variant 1 10 ug
$803.00

Plasmid Map for MR224033

Download sequence for

Cloning scheme for MR224033

Circular map for MR224033

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products