Otc (NM_008769) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR223979
Otc (Myc-DDK-tagged) - Mouse ornithine transcarbamylase (Otc), nuclear gene encoding mitochondrial protein
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Otc
Synonyms AI265390; Sf; spf
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223979 representing NM_008769
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTCTAATTTGAGGATCCTGCTCAACAATGCAGCTCTTAGAAAGGGTCACACTTCTGTGGTTCGAC
ATTTTTGGTGTGGGAAGCCAGTCCAAAGTCAAGTACAGCTGAAAGGCCGTGACCTCCTCACCTTGAAGAA
CTTCACAGGAGAGGAGATTCAGTACATGCTATGGCTCTCTGCAGATCTGAAATTCAGGATCAAGCAGAAA
GGAGAATATTTACCTTTATTGCAAGGGAAATCCTTAGGAATGATTTTTGAGAAAAGAAGTACTCGAACAA
GACTGTCCACAGAAACAGGCTTTGCTCTGCTGGGAGGACACCCTTCCTTTCTTACCACACAAGACATTCA
CTTGGGTGTGAATGAAAGTCTCACAGACACCGCTCGTGTCTTATCTAGCATGACAGATGCAGTGTTAGCT
CGAGTGTATAAACAATCAGATCTGGACACCCTGGCTAAAGAAGCATCCATCCCAATTGTCAATGGACTGT
CAGACTTGTATCATCCTATCCAGATCCTGGCTGATTACCTTACACTCCAGGAACACTATGGCTCTCTCAA
AGGTCTTACCCTCAGCTGGATAGGGGATGGGAACAATATCTTGCACTCTATCATGATGAGTGCTGCAAAA
TTCGGGATGCACCTTCAAGCAGCTACTCCAAAGGGTTATGAGCCAGATCCTAATATAGTCAAGCTAGCAG
AGCAGTATGCCAAGGAGAATGGTACCAAGTTGTCAATGACAAATGATCCACTGGAAGCAGCACGTGGAGG
CAATGTATTAATTACAGATACTTGGATAAGCATGGGACAAGAGGATGAGAAGAAAAAGCGTCTTCAAGCT
TTCCAAGGTTACCAGGTTACGATGAAGACTGCCAAAGTGGCTGCGTCTGACTGGACATTTTTACACTGTT
TGCCTAGAAAGCCAGAAGAAGTGGATGATGAAGTATTTTATTCTCCACGGTCATTAGTGTTCCCAGAGGC
AGAGAATAGAAAGTGGACAATCATGGCTGTCATGGTATCCCTGCTGACAGACTACTCACCTGTGCTCCAG
AAGCCAAAGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR223979 representing NM_008769
Red=Cloning site Green=Tags(s)

MLSNLRILLNNAALRKGHTSVVRHFWCGKPVQSQVQLKGRDLLTLKNFTGEEIQYMLWLSADLKFRIKQK
GEYLPLLQGKSLGMIFEKRSTRTRLSTETGFALLGGHPSFLTTQDIHLGVNESLTDTARVLSSMTDAVLA
RVYKQSDLDTLAKEASIPIVNGLSDLYHPIQILADYLTLQEHYGSLKGLTLSWIGDGNNILHSIMMSAAK
FGMHLQAATPKGYEPDPNIVKLAEQYAKENGTKLSMTNDPLEAARGGNVLITDTWISMGQEDEKKKRLQA
FQGYQVTMKTAKVAASDWTFLHCLPRKPEEVDDEVFYSPRSLVFPEAENRKWTIMAVMVSLLTDYSPVLQ
KPKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008769
ORF Size 1062 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008769.4, NP_032795.1
RefSeq Size 2265 bp
RefSeq ORF 1065 bp
Locus ID 18416
UniProt ID P11725
Cytogenetics X 4.66 cM
MW 40.2 kDa
Summary Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008769" in other vectors (4)
SKU Description Size Price
MC209128 Otc (untagged) - Mouse ornithine transcarbamylase (Otc), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$686.00
MG223979 Otc (tGFP-tagged) - Mouse ornithine transcarbamylase (Otc) nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$886.00
MR223979L3 Lenti ORF clone of Otc (Myc-DDK-tagged) - Mouse ornithine transcarbamylase (Otc), nuclear gene encoding mitochondrial protein 10 ug
$986.00
MR223979L4 Lenti ORF clone of Otc (mGFP-tagged) - Mouse ornithine transcarbamylase (Otc), nuclear gene encoding mitochondrial protein 10 ug
$986.00

Plasmid Map for MR223979

Download sequence for

Cloning scheme for MR223979

Circular map for MR223979

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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