Tst (NM_009437) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR223915
Tst (Myc-DDK-tagged) - Mouse thiosulfate sulfurtransferase, mitochondrial (Tst), nuclear gene encoding mitochondrial protein
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tst
Synonyms Rhodanese
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223915 representing NM_009437
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTACATCAGGTGCTCTACCGGGCGCTGGTATCCACCAAGTGGCTGGCGGAATCCATTCGGTCTGGCC
GCCTGGGGCCCAGCCTTCGGGTGCTGGACGCGTCTTGGTACTCACCCGGGACCCGCCAGGCACGCAAAGA
GTACCAGGAGCGACACGTGCCTGGCGCGTCCTTCTTTGATATAGAGGAATGCCGGGACACGACTTCGCCT
TATGAGATGATGCTGCCCAGTGAGGCCCACTTTGGGGACTATGTGGGCAACCTGGGCATAAGCAACGACA
CACATGTGGTGGTATACGATGGAGATGACCTGGGCAGCTTCTATGCACCCAGAGTCTGGTGGATGTTTCG
TGTGTTTGGCCACCGCACAGTGTCAGTGCTCAATGGTGGCTTCCGGAACTGGCTGAAGGAGGGCCACCCG
GTGACATCTGAGCCCTCGAGGCCAGAGCCAGCCGTTTTCAAAGCCACACTGAATCTCTCCCTGCTCAAGA
CCTATGAGCAGGTCCTAGAGAATCTCCAGTCTAAAAGGTTCCAGCTGGTGGACTCTCGGGCCCAAGGACG
GTACCTGGGCACACAACCGGAGCCGGATATAGTAGGACTAGACTCGGGCCACATCCGTGGCTCAGTCAAC
ATGCCCTTCATGGACTTCCTGACGAAAGATGGCTTCGAGAAGAGCCCAGAGGAGCTGCGTGCCATATTCC
AGGACAAGAAGGTGGACCTCTCACAGCCCCTCATTGCCACGTGCCGCAAGGGGGTCACTGCCTGTCACGT
TGCCCTGGCTGCCTACCTTTGTGGCAAACCCGATGTGGCTGTTTACGATGGCTCCTGGTCAGAGTGGTTC
CGCAGGGCACCCCCAGAGACCCGTGTGTCCCAGGGGAAGAGTGGGAAGGCC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR223915 representing NM_009437
Red=Cloning site Green=Tags(s)

MVHQVLYRALVSTKWLAESIRSGRLGPSLRVLDASWYSPGTRQARKEYQERHVPGASFFDIEECRDTTSP
YEMMLPSEAHFGDYVGNLGISNDTHVVVYDGDDLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHP
VTSEPSRPEPAVFKATLNLSLLKTYEQVLENLQSKRFQLVDSRAQGRYLGTQPEPDIVGLDSGHIRGSVN
MPFMDFLTKDGFEKSPEELRAIFQDKKVDLSQPLIATCRKGVTACHVALAAYLCGKPDVAVYDGSWSEWF
RRAPPETRVSQGKSGKA

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_009437
ORF Size 891 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009437.5
RefSeq Size 1096 bp
RefSeq ORF 894 bp
Locus ID 22117
UniProt ID P52196
Cytogenetics 15 37.47 cM
MW 33.5 kDa
Summary Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA (By similarity). Formation of iron-sulfur complexes and cyanide detoxification.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009437" in other vectors (4)
SKU Description Size Price
MC209598 Tst (untagged) - Mouse thiosulfate sulfurtransferase, mitochondrial (Tst), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$330.00
MG223915 Tst (tGFP-tagged) - Mouse thiosulfate sulfurtransferase mitochondrial (Tst) nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$500.00
MR223915L3 Lenti ORF clone of Tst (Myc-DDK-tagged) - Mouse thiosulfate sulfurtransferase, mitochondrial (Tst), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR223915L4 Lenti ORF clone of Tst (mGFP-tagged) - Mouse thiosulfate sulfurtransferase, mitochondrial (Tst), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR223915

Download sequence for

Cloning scheme for MR223915

Circular map for MR223915

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