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Lif (NM_001039537) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223901
Lif (Myc-DDK-tagged) - Mouse leukemia inhibitory factor (Lif), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lif
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223901 representing NM_001039537
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCAGATCAAGAATCAACTGGCACAGCTCAATGGCAGCGCCAATGCTCTCTTCATTTCCTATTACA
CAGCTCAAGGGGAGCCGTTTCCCAACAACGTGGAAAAGCTATGTGCGCCTAACATGACAGACTTCCCATC
TTTCCATGGCAACGGGACAGAGAAGACCAAGTTGGTGGAGCTGTATCGGATGGTCGCATACCTGAGCGCC
TCCCTGACCAATATCACCCGGGACCAGAAGGTCCTGAACCCCACTGCCGTGAGCCTCCAGGTCAAGCTCA
ATGCTACTATAGACGTCATGAGGGGCCTCCTCAGCAATGTGCTTTGCCGTCTGTGCAACAAGTACCGTGT
GGGCCACGTGGATGTGCCACCTGTCCCCGACCACTCTGACAAAGAAGCCTTCCAAAGGAAAAAGTTGGGT
TGCCAGCTTCTGGGGACATACAAGCAAGTCATAAGTGTGGTGGTCCAGGCCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223901 representing NM_001039537
Red=Cloning site Green=Tags(s)

MNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSA
SLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLG
CQLLGTYKQVISVVVQAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039537
ORF Size 474 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039537.2, NP_001034626.1
RefSeq Size 3798 bp
RefSeq ORF 477 bp
Locus ID 16878
UniProt ID P09056
Cytogenetics 11 2.94 cM
MW 18 kDa
Summary LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.UniProtKB/Swiss-Prot Function
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Documents
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"NM_001039537" in other vectors (4)
SKU Description Size Price
MC208888 Lif (untagged) - Mouse leukemia inhibitory factor (Lif), transcript variant 2, (10ug) 10 ug
$165.00
MG223901 Lif (tGFP-tagged) - Mouse leukemia inhibitory factor (Lif) transcript variant 2, (10ug) 10 ug
$350.00
MR223901L3 Lenti ORF clone of Lif (Myc-DDK-tagged) - Mouse leukemia inhibitory factor (Lif), transcript variant 2 10 ug
$450.00
MR223901L4 Lenti ORF clone of Lif (mGFP-tagged) - Mouse leukemia inhibitory factor (Lif), transcript variant 2 10 ug
$450.00

Plasmid Map for MR223901

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223901
Circular map for MR223901

file-alt Citations

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