Cebpd (NM_007679) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR223883
Cebpd (Myc-DDK-tagged) - Mouse CCAAT/enhancer binding protein (C/EBP), delta (Cebpd)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cebpd
Synonyms c/EBPdelta
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223883 representing NM_009456
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGCCGCGCTTTTCAGCCTGGACAGCCCGGTGCGCGGCACACCCTGGCCCACAGAACCCGCGGCCT
TCTACGAGCCAGGCAGGGTGGACAAGCCCGGCCGAGGGCCCGAGCCAGGGGATCTGGGGGAGCTGGGCTC
CACGACTCCTGCCATGTACGACGACGAGAGCGCCATCGACTTCAGCGCCTACATTGACTCCATGGCCGCC
GTGCCCACCCTAGAGCTGTGCCACGACGAACTCTTCGCCGACCTCTTCAACAGCAACCACAAAGCGGCCG
GCGCGGGCGGCCTGGAGCTGCTGCAGGGCGGCCCTACGCGACCCCCGGGTGTGGGGTCTGTCGCTAGGGG
GCCGCTCAAGCGCGAACCCGACTGGGGCGACGGCGACGCGCCGGGCTCCCTGCTGCCGGCGCAAGTGGCG
GTGTGCGCGCAGACAGTGGTGAGCTTGGCGGCCGCGGCTCAGCCCACTCCACCCACTTCGCCGGAGCCTC
CTCGAGGCAGCCCGGGGCCGAGCCTCGCGCCCGGCACAGTCCGAGAAAAGGGCGCGGGCAAGAGGGGTCC
GGACCGCGGCAGCCCGGAGTACCGGCAGCGGCGCGAGCGCAACAACATCGCTGTGCGCAAGAGCCGCGAC
AAGGCCAAGCGCCGCAACCAGGAGATGCAGCAGAAGCTGGTGGAGTTGTCGGCCGAGAACGAGAAGCTGC
ATCAGCGCGTGGAGCAGCTCACCCGGGACCTGGCTGGCCTCCGGCAGTTCTTCAAAAAACTGCCCAGCCC
GCCTTTCCTGCCGCCCACCGGCGCCGACTGCCGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR223883 representing NM_007679
Red=Cloning site Green=Tags(s)

MSAALFSLDSPVRGTPWPTEPAAFYEPGRVDKPGRGPEPGDLGELGSTTPAMYDDESAIDFSAYIDSMAA
VPTLELCHDELFADLFNSNHKAAGAGGLELLQGGPTRPPGVGSVARGPLKREPDWGDGDAPGSLLPAQVA
VCAQTVVSLAAAAQPTPPTSPEPPRGSPGPSLAPGTVREKGAGKRGPXXRGSPEYRQRRERNNIAVRKSR
DKAKRRNQEMQQKLVELSAENEKLHQRVEQLTRDLAGLRQFFKKLPSPPFLPPTGADCR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_007679
ORF Size 804 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007679.1, NM_007679.2, NM_007679.3, NM_007679.4, NP_031705.3
RefSeq Size 2260 bp
RefSeq ORF 807 bp
Locus ID 12609
UniProt ID Q00322
Cytogenetics 16 10.09 cM
MW 29.1 kDa
Summary Transcription activator that recognizes two different DNA motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers (PubMed:7594592, PubMed:19641492). Important transcription factor regulating the expression of genes involved in immune and inflammatory responses. Transcriptional activator that enhances IL6 transcription alone and as heterodimer with CEBPB (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007679" in other vectors (4)
SKU Description Size Price
MC208277 Cebpd (untagged) - Mouse CCAAT/enhancer binding protein (C/EBP), delta (Cebpd), (10ug) 10 ug
$480.00
MG223883 Cebpd (tGFP-tagged) - Mouse CCAAT/enhancer binding protein (C/EBP) delta (Cebpd), (10ug) 10 ug
$650.00
MR223883L3 Lenti ORF clone of Cebpd (Myc-DDK-tagged) - Mouse CCAAT/enhancer binding protein (C/EBP), delta (Cebpd) 10 ug
$750.00
MR223883L4 Lenti ORF clone of Cebpd (mGFP-tagged) - Mouse CCAAT/enhancer binding protein (C/EBP), delta (Cebpd) 10 ug
$750.00

file-alt Citations

Co-occupancy identifies transcription factor co-operation for axon growth
Venkatesh, Mehra, Wang et al
Nat Commun (2021) 12 (1), 2555 DOI: 10.1038/s41467-021-22828-3
Cited by 21
AI Summary: OriGene contributed to this study by providing constructs for candidate transcription factor genes, which were used to test their cooperative effects on axon growth.
Snippet: tch had genes belonging to one functional subnetwork to identify Klf6 synergizers by functional category. Cloning and virus production Constructs for candidate genes were purchased from Dharmacon or Origene, and relevant accession numbers for all 62 candidate TFs are summarized in Supplementary Data 2. mScarlet was a gift from Dorus Gadella (RRID:Addgene_85044), pAAV-CAG-tdTomato (codon diversif;...
FigureFigureFigure
Showing 1-1 of 1 papers.
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