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Cep76 (NM_001081073) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223755
Cep76 (Myc-DDK-tagged) - Mouse centrosomal protein 76 (Cep76)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $614.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cep76
Synonyms 6230425F05Rik; 9630013E05
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223755 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCTGCCTCCGGAGAAAGCCTCGGAGCTGAAGCAGCTCATCCACCAGCAGCTGAGCAAGATGGATG
TCCATGGCAGAATAAGAGAAATTCTTGCAGAGACTATACGAGAAGAATTAGCACCGGATCAACAACATTT
ATCAACAGAAGATCTGATCAAAGCTCTAAGACGTCGAGGAATCATTGATGATGTAATGAAAGAACTTAAT
TTTGTGACTGATAGTGTTGATCAAGAACTCCCTTCTTCTCCAAAGCAAACCGTTGGTTTTGATAAGCAGT
CAACATTAAAAAAAACTAATGTTGATCCAACACGGAGGTATCTTTACCTTCAGGTTCTGGGTGGAAAAGC
TTTCTTGGAACATCTGCAAGAACCTGAGCCTTTGCCTGGACAGATTTGCTCGACTTTCACTTTATGTTTG
CACTATCGGAACCAACGATTTCGCTCCAAACCTGTACCCTGCGCTTGTGAGCCAGATTTCCATGACGGCT
TTTTGCTTGAAGTTCACAGAGAAAGTTTAGGTGATGGAACTAGAATGGCTGATTCAACAACAATGTTATC
AATAAGTGATCCAATTCACATGGTACTAATCAAAACAGACATATTTGGTGAGACCACTTTAGTTGCGTCC
TATTTCCTGGAATGGAGATCAGTTTTGGGCTCAGAAAATGGAGTGACCAATCTGACTGTGGAGCTCATGG
GTGTAGGCACAGAATCAAAAGTTTCCGTGGGAATTTTAAATATAAAACTTGAAATGTACCCACCGCTCAG
CCAAACGCTGTCTCAGGAGGTAGTGAACACACAGCTTGCCTTGGAACGTCAGAAAACTGCAGAGAAAGAG
CGATTATTTCTCGTCTATGCTAAGCAGTGGTGGAGGGAGTATTTGCAAATTCGACCTTCTCACAACTCAA
GGCTGGTCAAGATTTTTGCCCAGGATGAGAATGGGATAAATAGACCAGTCTGTTCCTACGTTAAGCCACT
TCGAGCTGGACGACTTCTGGATACTCCGAGGCAAGCGGCTCGATTTGTTAATGTCCTTGGTTATGAACGA
GCCCCTGTTATTGGAGGAGGTGGCAAGCAGGAACAGTGGTGCACTCTGCTGGCTTTTCTCTGTAGAAACA
AGGGTGATTGTGAGGACCACGCTAACCTGCTGTGCAGCCTTCTTCTTGGCTATGGACTGGAGGCCTTTGT
CTGTGTGGGGACTAAGGCCAAGGGGGCGCCGCATGCCTGGGTGATGACCTGTGGGACTGATGGCACCATC
ATGTTTTGGGAGAGTTTAACAGGGCACAGGTACATCCACAAGCCTACCAATCCTGACGGACCTCCACTTG
CTGAGCAGCCCAAACCACTGTACCCATATCGAACAATTGGTTGTGTTTTCAATCATCAGATGTTCCTGGG
AAACTGTCAGCCCTCAGATGCAGTAGAGACCTGCATATTTGATTTGAATGATGAGTCCAAGTGGAAACCC
ATGAGCGAAGAAGCAATTAAGTCTGTGTGTGCTCCAGGTGCTACTACCTCCCTCCCTCCCTTCCCACCTC
TGTGTGCGTCCACGATTGACGCCTCGGTCACCAGCAATGAAATCGAAATGCAGCTGAGGCTTCTGGTGTC
GGAACACAGAAAGGACCTTGGCCTCACCACTGTCTGGGAAGACCAGCTCTCCTATCTTTTGTCACCAGCT
CTGGCCTCGTATGAGTTTGAGCGAACAACAAGCATATCTGCAGGCAATGAAGAATTTCAGGATGCCATCA
GGAGGGCTGTACCTGACGGGCACACATTTAAAGGCTTCCCCATACATTTTGTGTATAGAAATGCAAGGCG
TGCATTTGCCACTTGTCTTCGGTCTCCTTTTTGTGAAGAAATAATCTGTTGTCGTGGAGACCAGGTGCGA
CTGGCAGTTCGCGTTCGAGTGTTTACCTACCCTGAGTCTGCATGTGCTGTTTGGATCATGTTTGCTTGTA
AATATCGCTCAGTATTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223755 protein sequence
Red=Cloning site Green=Tags(s)

MSLPPEKASELKQLIHQQLSKMDVHGRIREILAETIREELAPDQQHLSTEDLIKALRRRGIIDDVMKELN
FVTDSVDQELPSSPKQTVGFDKQSTLKKTNVDPTRRYLYLQVLGGKAFLEHLQEPEPLPGQICSTFTLCL
HYRNQRFRSKPVPCACEPDFHDGFLLEVHRESLGDGTRMADSTTMLSISDPIHMVLIKTDIFGETTLVAS
YFLEWRSVLGSENGVTNLTVELMGVGTESKVSVGILNIKLEMYPPLSQTLSQEVVNTQLALERQKTAEKE
RLFLVYAKQWWREYLQIRPSHNSRLVKIFAQDENGINRPVCSYVKPLRAGRLLDTPRQAARFVNVLGYER
APVIGGGGKQEQWCTLLAFLCRNKGDCEDHANLLCSLLLGYGLEAFVCVGTKAKGAPHAWVMTCGTDGTI
MFWESLTGHRYIHKPTNPDGPPLAEQPKPLYPYRTIGCVFNHQMFLGNCQPSDAVETCIFDLNDESKWKP
MSEEAIKSVCAPGATTSLPPFPPLCASTIDASVTSNEIEMQLRLLVSEHRKDLGLTTVWEDQLSYLLSPA
LASYEFERTTSISAGNEEFQDAIRRAVPDGHTFKGFPIHFVYRNARRAFATCLRSPFCEEIICCRGDQVR
LAVRVRVFTYPESACAVWIMFACKYRSVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081073
ORF Size 1977 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081073.1, NP_001074542.1
RefSeq Size 3979 bp
RefSeq ORF 1980 bp
Locus ID 225659
UniProt ID Q0VEJ0
Cytogenetics 18 E1
MW 74.3 kDa
Summary Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001081073" in other vectors (4)
SKU Description Size Price
MC220202 Cep76 (untagged) - Mouse centrosomal protein 76 (Cep76), (10ug) 10 ug
$676.00
MG223755 Cep76 (tGFP-tagged) - Mouse centrosomal protein 76 (Cep76), (10ug) 10 ug
$814.00
MR223755L3 Lenti ORF clone of Cep76 (Myc-DDK-tagged) - Mouse centrosomal protein 76 (Cep76) 10 ug
$914.00
MR223755L4 Lenti ORF clone of Cep76 (mGFP-tagged) - Mouse centrosomal protein 76 (Cep76) 10 ug
$914.00

Plasmid Map for MR223755

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223755
Circular map for MR223755

file-alt Citations

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