T (NM_009309) Mouse Tagged ORF Clone
$289.00
$457.00
2 Weeks*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | T |
| Synonyms | Bra; cou; D17Mit170; Low; Lr; me75; T1; Tl2; Tl3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009309 |
| ORF Size | 1308 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009309.2, NP_033335.1 |
| RefSeq Size | 2046 bp |
| RefSeq ORF | 1311 bp |
| Locus ID | 20997 |
| UniProt ID | P20293 |
| Cytogenetics | 17 4.92 cM |
| MW | 47.9 kDa |
| Summary | Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation (PubMed:7588606). Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site (PubMed:8344258, PubMed:7588606).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009309" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC215721 | T (untagged) - Mouse brachyury (T), (10ug) | 10 ug |
$503.00
|
|
| MG223752 | T (tGFP-tagged) - Mouse brachyury (T), (10ug) | 10 ug |
$657.00
|
|
| MR223752L3 | Lenti ORF clone of T (Myc-DDK-tagged) - Mouse brachyury (T) | 10 ug |
$757.00
|
|
| MR223752L4 | Lenti ORF clone of T (mGFP-tagged) - Mouse brachyury (T) | 10 ug |
$757.00
|
|
Citations
Glycosylphosphatidylinositol biosynthesis and remodeling are required for neural tube closure, heart development, and cranial neural crest cell survival
Lukacs, Roberts, Chatuverdi et al
Elife (2019) 8 DOI: 10.7554/eLife.45248
Lukacs, Roberts, Chatuverdi et al
Elife (2019) 8 DOI: 10.7554/eLife.45248
AI Summary: In this study, OriGene’s mouse Piga plasmid (#MR222212), mouse Pgap2 plasmid (#MR2031890), and mouse Pigp plasmid (#MR216742) were used as recombinant DNA reagents to investigate the roles of GPI biosynthesis genes in neural tube closure, heart development, and neural crest cell survival.
Snippet: Software, algorithm Benchling sgRNA design software Benchling, San Fransisco CA RRID:SCR_013955 [https://scicrunch.org/resolver/SCR_013955] Recombinant DNA reagent mouse Piga plasmid Origene Origene: _#_MR222212 Recombinant DNA reagent mouse Pgap2 plasmid Origene Origene: #MR2031890 Recombinant DNA reagent mouse Pigp plasmid Origene Origene: #MR216742 Recomb;... compbio/CSBB-v3.0 RRID:SCR_017234 Software, algorithm Benchling sgRNA design software Benchling, San Fransisco CA RRID:SCR_013955 Recombinant DNA reagent mouse Piga plasmid Origene Origene: #MR222212 Recombinant DNA reagent mouse Pgap2 plasmid Origene Origene: #MR2031890 Recombinant DNA reagent mouse Pigp plasmid Origene Origene: #MR216742
Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle
Quattrocelli, Swinnen, Giacomazzi et al
J. Clin. Invest. (2015) 125 (12), 4463-4482 DOI: 10.1172/JCI82735
Quattrocelli, Swinnen, Giacomazzi et al
J. Clin. Invest. (2015) 125 (12), 4463-4482 DOI: 10.1172/JCI82735
Cited by 1
AI Summary: OriGene’s pCMV6-Entry plasmid carrying the brachyury gene was used for transient overexpression in mesodermal iPSC-derived progenitors (MiPs), enabling the study of their differentiation and regenerative potential in cardiac and skeletal muscle.
Snippet: … Transient overexpression of brachyury (pCMV6-Entry;Origene) and Pax7 (as mentioned above) in MiPs was achieved by Lipofectamine-based transfection of small amounts of plasmid, i.e., 100 ng brachyury-bearing and 120 ng Pax7-bearing plasmids per 24-well plate (cells transfected with the empty vectors were used as a control)
Glycosylphosphatidylinositol Biosynthesis and Remodeling are Required for Neural Crest Cell, Cardiac and Neural Development
Lukacs, Roberts, Chaturvedi et al
bioRxiv (2019)
Lukacs, Roberts, Chaturvedi et al
bioRxiv (2019)
Showing 1-3 of 3 papers.
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