Vstm2b (NM_021387) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR223744
Vstm2b (Myc-DDK-tagged) - Mouse V-set and transmembrane domain containing 2B (Vstm2b)
$289.00 $300.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vstm2b
Synonyms 2900093B09Rik; AB030198
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223744 representing NM_021387
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACAGCGGAACCGGCTCGGCGCCCTCGGATACCTGCTACCTCTGCTACTGCACAGCCTGCTGCTCT
TCGTGGCCGACGCTACATTCACCGAAGTCCCCAAAGATGTAACCGTGCGAGAGGGTGACGATATCGAAAT
GCCCTGCGCGTTCCGGGCCAGCGGAGCCACCTCTTACTCGCTGGAGATTCAGTGGTGGTACCTCAAGGAG
CCTCCCCGGGAGCTGTTACACGAGCTGGCGCTCAGCGTGCCGGGCGCCCGGAGCAAGGTAACAAATAAGG
ATGCAACTAAAATCAGCACCGTGCGAGTCCAGGGCAATGACATCTCACACCGGCTTCGGCTGTCCGCCGT
ACGATTGCAGGACGAAGGTGTTTATGAATGTCGCGTTTCGGACTACAGCGATGATGACACTCAGGAGCAC
AAGGCCCAGGCATTGCTGCGTGTGCTATCGCGCTTCGCGCCGCCCAACATGCAAGCTGCAGAGGCAGTGT
CCCACATTCAGAGCAGCGGCCCGCGTCGCCACGGAGCCTCCAGTGCTGTCAGCTCCAACAACGCAGGCGC
TGCTGTCCGCACCACCTCTGAAACCAGCCACGATGACAAGAACCCACCTCCTGGGAGCCCTCCCGCTGGA
TCAGGAGTTCCTGAGGCCGCCGCTGCCGCTGCCTCCGCGACCCACACAGCCACCACAACTGCTGCTGCAG
CTGCTGCTTCTTCATCAGCATCACCACCATCAGGGCAGGCTGTCCTTCTGCGCCAGAGGCACGGCTCCGG
TACCGGCCCAGGCTACAGTGCCGATCCTCTGCTGTCTCTGCTCTTGTTAGCACTACATAAGTTCCTGCAC
CCACTTCTGGGCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR223744 representing NM_021387
Red=Cloning site Green=Tags(s)

MEQRNRLGALGYLLPLLLHSLLLFVADATFTEVPKDVTVREGDDIEMPCAFRASGATSYSLEIQWWYLKE
PPRELLHELALSVPGARSKVTNKDATKISTVRVQGNDISHRLRLSAVRLQDEGVYECRVSDYSDDDTQEH
KAQALLRVLSRFAPPNMQAAEAVSHIQSSGPRRHGASSAVSSNNAGAAVRTTSETSHDDKNPPPGSPPAG
SGVPEAAAAAASATHTATTTAAAAAASSSASPPSGQAVLLRQRHGSGTGPGYSADPLLSLLLLALHKFLH
PLLGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021387
ORF Size 855 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021387.3, NP_067362.1
RefSeq Size 1455 bp
RefSeq ORF 858 bp
Locus ID 58188
UniProt ID Q9JME9
Cytogenetics 7 B3
MW 30.6 kDa
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Documents
Resources
"NM_021387" in other vectors (4)
SKU Description Size Price
MC210162 Vstm2b (untagged) - Mouse V-set and transmembrane domain containing 2B (Vstm2b), (10ug) 10 ug
$330.00
MG223744 Vstm2b (tGFP-tagged) - Mouse V-set and transmembrane domain containing 2B (Vstm2b), (10ug) 10 ug
$500.00
MR223744L3 Lenti ORF clone of Vstm2b (Myc-DDK-tagged) - Mouse V-set and transmembrane domain containing 2B (Vstm2b) 10 ug
$600.00
MR223744L4 Lenti ORF clone of Vstm2b (mGFP-tagged) - Mouse V-set and transmembrane domain containing 2B (Vstm2b) 10 ug
$600.00

Plasmid Map for MR223744

Download sequence for

Cloning scheme for MR223744

Circular map for MR223744

file-alt Citations

Sexually dimorphic control of excitatory synapse maintenance in ventral hippocampal circuits by Neurexin2α ligand Vstm2b
Chofflet, Chofflet, Inagaki et al
Nature Communications (2026)
Snippet: Full length cDNAs encoding mouse Vstm2a (#MR202852), Vstm2b (#MR223744) and Vstm2l (#MR202504) were purchased from Origene. Mouse Vstm2a, Vstm2b, and Vstm2l lacking the signal peptide were cloned in frame with the TrkC signal peptide with an N-terminal Myc, HA, or
Showing 1-1 of 1 papers.
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