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Gpr151 (NM_181543) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223707
Gpr151 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 151 (Gpr151)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$289.00 $686.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr151
Synonyms C130082O03Rik; GalRL; nGPCR-2037; PGR7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223707 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAAGGCAATGCTGAGAGCTGGGTTTGCCGACACCAATTCCAGCAACATGAACGAGTCGTTTGCTC
GCCTCCACTTTGCAGGAGGCTACCTGCCGTCTGACTCCAAGGACTGGAGGACCATCATTCCGTCTCTCTT
GATGGCCGTGTGCCTGGTGGGTCTCGTGGGAAACCTGTGTGTGATTGGCATTCTCCTTCATGGCGTTTGG
AAAAGAAAGCCATCCACGATCCACTCCCTGATTCTGAATCTAAGCCTGGCTGACTTCTCTCTCCTGCTCT
TCTCTGCACCTGTCCGAGCGGCAGCATACTCCAAGGGCGTTTGGGATCTCGGCTGGTTCATCTGCAAGTC
CTCTGACTGGTTCACCCACGTGTGCATGGCAGCCAAGAGCTTGACATTTGTTGTGGTAGCCAAAGCATGC
TTCGCGTATGCAAGTGACCCAGCCAAGCAAGAGAGTATCCACAGCCGCACCATCTGGTCAGTGCTGGCGG
GCATCTGGGTTGTAGCCAGCCTGCTTCCCCTGCCAGAATGGCTCTTTAGCACCACCAGACGTCACGCAGG
TGTGGAAATGTGCCTCGTGGATGTGCCAGCTGTGGCAGAAGAATTCATGTCCATGTTCGGTAAGCTCTAC
CCTCTGCTGGTGTTCTGCCTTCCTTTACTTCTGGCTGGCGTTTATTTCTGGCGAGCTTATGACCAATGTA
AGACACGATGTACGAAAACTCGAAATCTGAGAGACCAGATGCGTTCAAAGCAACTCACAGTGATGCTGCT
AAGCACTGCAATCATCTCTGCTCTTCTGTGGCTCCCAGAGTGGATAGCCTGGCTGTGGGTATGGCATGTG
AAGGCTGGAGGGCCGATGCCACCACAGGGGTTCATAGCCCTGTCTCAAGTTCTCATGTTTTTCACCTCCA
CAGCAAACCCTCTCATTTTTCTAGTTATGTCTGAAGAGTTCAAGGCAGGCTTGAAAGGCCTATGGAAATG
GATGATAACCAGAAAGCCAGCAGTTACTTCAGAGGTTCAGGAGGCACCTGCTGGAAACACAGAGGCCCTT
CCCGGAAAGGCTCCATCACCAGAGACCCAAACATGCATCCTAGACACAGACGGACGTGGGTCTCCTGATG
ACAGCAAAGAGAAATCTGGTAAGGTGGTGGCTCCCATCCTCCCTGACGTGGAGCAGTTTTGGCACGAGAG
GGATGCTGTCCCTTCAGCACAAGACAATGACCCTATACCCTGGGAACACGAAGGCCAAGAGACAGAGGGC
TGCAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223707 protein sequence
Red=Cloning site Green=Tags(s)

MGKAMLRAGFADTNSSNMNESFARLHFAGGYLPSDSKDWRTIIPSLLMAVCLVGLVGNLCVIGILLHGVW
KRKPSTIHSLILNLSLADFSLLLFSAPVRAAAYSKGVWDLGWFICKSSDWFTHVCMAAKSLTFVVVAKAC
FAYASDPAKQESIHSRTIWSVLAGIWVVASLLPLPEWLFSTTRRHAGVEMCLVDVPAVAEEFMSMFGKLY
PLLVFCLPLLLAGVYFWRAYDQCKTRCTKTRNLRDQMRSKQLTVMLLSTAIISALLWLPEWIAWLWVWHV
KAGGPMPPQGFIALSQVLMFFTSTANPLIFLVMSEEFKAGLKGLWKWMITRKPAVTSEVQEAPAGNTEAL
PGKAPSPETQTCILDTDGRGSPDDSKEKSGKVVAPILPDVEQFWHERDAVPSAQDNDPIPWEHEGQETEG
CN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181543
ORF Size 1266 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181543.2
RefSeq Size 1633 bp
RefSeq ORF 1269 bp
Locus ID 240239
UniProt ID Q7TSN6
Cytogenetics 18 B3
MW 46.8 kDa
Summary Proton-sensing G-protein coupled receptor.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_181543" in other vectors (4)
SKU Description Size Price
MC213097 Gpr151 (untagged) - Mouse G protein-coupled receptor 151 (Gpr151), (10ug) 10 ug
$686.00
MG223707 Gpr151 (tGFP-tagged) - Mouse G protein-coupled receptor 151 (Gpr151), (10ug) 10 ug
$489.00 $886.00
MR223707L3 Lenti ORF clone of Gpr151 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 151 (Gpr151) 10 ug
$986.00
MR223707L4 Lenti ORF clone of Gpr151 (mGFP-tagged) - Mouse G protein-coupled receptor 151 (Gpr151) 10 ug
$986.00

Plasmid Map for MR223707

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223707
Circular map for MR223707

file-alt Citations

Targeted disruption of the orphan receptor Gpr151 does not alter pain-related behaviour despite a strong induction in dorsal root ganglion expression in a model of neuropathic pain
Holmes, Kerr, Chen et al
Mol. Cell. Neurosci. (2016) 78, 35-40 DOI: 10.1016/j.mcn.2016.11.010
AI Summary: OriGene’s Gpr151 plasmid was used to transiently transfect ND7/23 cells, enabling the study of Gpr151 function in the context of neuropathic pain in this research.
Snippet: ND7/23 cells (ECACC 92090903, (Wood et al., 1990)) were transiently transfected with a Gpr151 plasmid (OriGene, USA) using Lipofectamine 2000 according to the manufacturer's instructions (Invitrogen), and maintained in normal culture medium overnight (high glucose ... Cite SaveSaving
Showing 1-1 of 1 papers.
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