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Alg6 (NM_001081264) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223659
Alg6 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) (Alg6)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $472.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Alg6 or NM_001081264"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Alg6
Synonyms E230028F23Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223659 representing NM_001081264
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGCTGGCCGTGGATGGCAGTCGTGGTCTTACTAGGACTTACGGTGCGGTGGACGGTCTCTCTCA
GTTCCTACTCAGGTGCTGGAAAACCACCTATGTTTGGTGATTATGAAGCTCAGAGGCACTGGCAGGAAAT
TACCCTTAATTTACCAGTCAAACAGTGGTATTTTAACAGTAGTGATAACAATTTACTGTACTGGGGATTG
GATTACCCACCTCTTACGGCCTATCATAGCCTCTTATGTGCATATGTGGCAAAGTTTATAAACCCAGACT
GGGTTGCTCTCCACACATCACGAGGCTATGAGAGTCAGGCACACAAGCTCTTCATGCGTGCCACAGTTCT
TGCTGCTGATTTGCTCATTTACGTACCCGCGGTGCTTTTGTACTGTTACTCCTTAAAAGAAATCTCACCT
AAGAGAAAGATTGCTAGTGCGTTATGCATATTGCTGTATCCAGGTCTTATTCTTATCGACTATGGACATT
TTCAATATAATTCTGTGAGTCTTGGCTTTGCGTTGTGGGGTGTTCTTGGAGTATCTTGGGACTGGGACCT
GCTAGGGTCACTGGCATTTTGCTTAGCTCTAAATTATAAGCAGATGGAACTTTACCACTCCCTGCCATTT
TTTTGCTTTTTACTTGGCAAGTGTTTTAAAAAAGGCCTCAAAGGAAAGGGGCTTGCATTGTTCATTAGGA
TAGCCTGTACTGTCCTGGCTTCTTTCCTTCTGTGTTGGCTGCCGTTCTTGACAGAAAGGGAGCATGCCCT
GCAGGTTGTTAGAAGACTCTTCCCTGTTGACCGTGGACTGTTTGAGGATAAAGTCGCTAATATTTGGTGC
AGCGTTAATGTCTTTCTGAAGATTAAGGACACTTTGCCTCGCCACATCCAGATAGCGATCAGCTTTTGCT
TTACACTTTTGAGCTTGCTTCCTGCATGTATAAAACTGACAGTTCGACCCTCTTGTAAAGGATTCAGATT
TACTCTGGTTAGCTGTGCGCTATCATTCTTTCTGTTTTCTTTCCAAGTACATGAAAAGTCCATTCTCTTG
GTATCATTACCAGTTTGCTTAGTTTTAACTGAAATTCCTTTCATGTCAACCTGGTTTTTACTAGTTTCAA
CATTTAGCATGCTGCCTCTTCTATTAAAGGATGAACTCCTGCTGCCCTCGGTTGTGACAGTGATGGCATT
TGTAATAGCTTGTGGCACTTTCTTTCCAATGTTGGAGAACACTTCCGAAGAACAACTGCAGTTGAAGTCC
TTTGCTGTCTCTGTAAGGAGGCACCTTCCGGGCTTCACATTTTTGCCCAGAATTATGCAGTGTTTGTTTC
TTTCTTCAGTCATCACCATGGTCCTCCTGACGATTTTGAGTGTAACACTGGACCCTCCTCAGAAATTACC
AGACTTGTTTCCTGTACTTATATGTTTTGTTTCCTGTGTAAATTTTGTATTCTTCTTGGTATACTTTAAT
ATTGTAATCATGTGGGATTCCAAAAATGGAAGAAATCGGAAGAAAATTGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223659 representing NM_001081264
Red=Cloning site Green=Tags(s)

MESWPWMAVVVLLGLTVRWTVSLSSYSGAGKPPMFGDYEAQRHWQEITLNLPVKQWYFNSSDNNLLYWGL
DYPPLTAYHSLLCAYVAKFINPDWVALHTSRGYESQAHKLFMRATVLAADLLIYVPAVLLYCYSLKEISP
KRKIASALCILLYPGLILIDYGHFQYNSVSLGFALWGVLGVSWDWDLLGSLAFCLALNYKQMELYHSLPF
FCFLLGKCFKKGLKGKGLALFIRIACTVLASFLLCWLPFLTEREHALQVVRRLFPVDRGLFEDKVANIWC
SVNVFLKIKDTLPRHIQIAISFCFTLLSLLPACIKLTVRPSCKGFRFTLVSCALSFFLFSFQVHEKSILL
VSLPVCLVLTEIPFMSTWFLLVSTFSMLPLLLKDELLLPSVVTVMAFVIACGTFFPMLENTSEEQLQLKS
FAVSVRRHLPGFTFLPRIMQCLFLSSVITMVLLTILSVTLDPPQKLPDLFPVLICFVSCVNFVFFLVYFN
IVIMWDSKNGRNRKKIE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081264
ORF Size 1521 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081264.1, NP_001074733.1
RefSeq Size 3114 bp
RefSeq ORF 1524 bp
Locus ID 320438
UniProt ID Q3TAE8
Cytogenetics 4 C6
MW 58.3 kDa
Summary Adds the first glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Man(9)GlcNAc(2)-PP-Dol (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_001081264" in other vectors (4)
SKU Description Size Price
MC217219 Alg6 (untagged) - Mouse asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) (Alg6), (10ug) 10 ug
$520.00
MG223659 Alg6 (tGFP-tagged) - Mouse asparagine-linked glycosylation 6 homolog (yeast alpha-13-glucosyltransferase) (Alg6), (10ug) 10 ug
$672.00
MR223659L3 Lenti ORF clone of Alg6 (Myc-DDK-tagged) - Mouse asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) (Alg6) 10 ug
$772.00
MR223659L4 Lenti ORF clone of Alg6 (mGFP-tagged) - Mouse asparagine-linked glycosylation 6 homolog (yeast, alpha-1,3,-glucosyltransferase) (Alg6) 10 ug
$772.00

Plasmid Map for MR223659

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223659
Circular map for MR223659

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