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Tshb (NM_009432) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223556
Tshb (Myc-DDK-tagged) - Mouse thyroid stimulating hormone, beta subunit (Tshb), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tshb
Synonyms MGC151206; MGC151208
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223556 representing NM_009432
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCTGCCGTCCTCCTCTCCGTGCTTTTTGCTCTTGCTTGTGGGCAAGCAGCATCCTTTTGTATTC
CCACTGAGTATACAATGTACGTGGATAGGAGAGAGTGTGCCTACTGCCTGACCATCAACACCACCATCTG
TGCTGGGTATTGTATGACACGGGATATCAATGGCAAACTGTTTCTTCCCAAATATGCACTCTCTCAGGAT
GTCTGTACATACAGAGACTTCATCTACAGAACGGTGGAAATACCAGGATGCCCGCACCATGTTACTCCTT
ATTTCTCCTTCCCTGTCGCCGTAAGCTGCAAGTGTGGCAAGTGTAATACTGACAACAGTGACTGCATACA
CGAGGCTGTCAGAACCAACTACTGCACCAAGCCGCAGTCTTTCTATCTGGGGGGATTTTCTGTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223556 representing NM_009432
Red=Cloning site Green=Tags(s)

MSAAVLLSVLFALACGQAASFCIPTEYTMYVDRRECAYCLTINTTICAGYCMTRDINGKLFLPKYALSQD
VCTYRDFIYRTVEIPGCPHHVTPYFSFPVAVSCKCGKCNTDNSDCIHEAVRTNYCTKPQSFYLGGFSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009432
ORF Size 414 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009432.1
RefSeq Size 645 bp
RefSeq ORF 417 bp
Locus ID 22094
Cytogenetics 3 45.25 cM
MW 15.8 kDa
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"NM_009432" in other vectors (4)
SKU Description Size Price
MC209595 Tshb (untagged) - Mouse thyroid stimulating hormone, beta subunit (Tshb), transcript variant 1, (10ug) 10 ug
$165.00
MG223556 Tshb (tGFP-tagged) - Mouse thyroid stimulating hormone beta subunit (Tshb) transcript variant 1, (10ug) 10 ug
$350.00
MR223556L3 Lenti ORF clone of Tshb (Myc-DDK-tagged) - Mouse thyroid stimulating hormone, beta subunit (Tshb), transcript variant 1 10 ug
$450.00
MR223556L4 Lenti ORF clone of Tshb (mGFP-tagged) - Mouse thyroid stimulating hormone, beta subunit (Tshb), transcript variant 1 10 ug
$450.00

Plasmid Map for MR223556

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223556
Circular map for MR223556

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