Hopx (NM_175606) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR223455
Hopx (Myc-DDK-tagged) - Mouse HOP homeobox (Hopx), transcript variant 1
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hopx
Synonyms 1110018K11Rik; 1200015P04Rik; 2300002F06Rik; AI848177; AW490897; Cameo; Hdop; Hod; Hop
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223455 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCGCAGACCGCGAGCGGCCCCACGGAGGACCAGGTGGAGATCCTGGAGTACAACTTCAACAAGG
TCAACAAGCACCCGGACCCCACCACGCTGTGCCTCATCGCAGCCGAGGCGGGTCTCACGGAGGAGCAGAC
GCAGAAATGGTTTAAGCAGCGCCTGGCAGAGTGGCGGCGGTCAGAAGGCTTGCCTTCGGAATGCAGATCT
GTTACGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR223455 protein sequence
Red=Cloning site Green=Tags(s)

MSAQTASGPTEDQVEILEYNFNKVNKHPDPTTLCLIAAEAGLTEEQTQKWFKQRLAEWRRSEGLPSECRS
VTD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175606
ORF Size 219 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175606.3, NP_783199.1
RefSeq Size 1265 bp
RefSeq ORF 222 bp
Locus ID 74318
UniProt ID Q8R1H0
Cytogenetics 5 C3.3
MW 8.3 kDa
Summary Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (PubMed:12297045, PubMed:12297046). Acts as a co-chaperone for HSPA1A and HSPA1B chaperone proteins and assists in chaperone-mediated protein refolding (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175606" in other vectors (5)
SKU Description Size Price
MC201799 Hopx (untagged) - Mouse HOP homeobox (Hopx), transcript variant 1, (10ug) 10 ug
$150.00
MG200084 Hopx (tGFP-tagged) - Mouse homeobox only domain (Hod) 10 ug
$350.00
MG223455 Hopx (tGFP-tagged) - Mouse HOP homeobox (Hopx) transcript variant 1, (10ug) 10 ug
$350.00
MR223455L3 Lenti ORF clone of Hopx (Myc-DDK-tagged) - Mouse HOP homeobox (Hopx), transcript variant 1 10 ug
$450.00
MR223455L4 Lenti ORF clone of Hopx (mGFP-tagged) - Mouse HOP homeobox (Hopx), transcript variant 1 10 ug
$450.00

Plasmid Map for MR223455

Download sequence for

Cloning scheme for MR223455

Circular map for MR223455

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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