Cdc27 (NM_145436) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR223434
Cdc27 (Myc-DDK-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27)
$589.00
$755.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cdc27 |
| Synonyms | AI452358; APC3; BC023187 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_145436 |
| ORF Size | 2475 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_145436.2, NP_663411.2 |
| RefSeq Size | 5783 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 217232 |
| UniProt ID | A2A6Q5 |
| Cytogenetics | 11 67.79 cM |
| MW | 92.3 kDa |
| Summary | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_145436" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC221891 | Cdc27 (untagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27), (10ug) | 10 ug |
$832.00
|
|
| MG223434 | Cdc27 (tGFP-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27), (10ug) | 10 ug |
$955.00
|
|
| MR223434L3 | Lenti ORF clone of Cdc27 (Myc-DDK-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27) | 10 ug |
$1,055.00
|
|
| MR223434L4 | Lenti ORF clone of Cdc27 (mGFP-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27) | 10 ug |
$1,055.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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