Neu3 (NM_016720) Mouse Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Neu3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_016720 |
| ORF Size | 1254 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_016720.1 |
| RefSeq Size | 3341 bp |
| RefSeq ORF | 1257 bp |
| Locus ID | 50877 |
| UniProt ID | Q9JMH7 |
| Cytogenetics | 7 E1 |
| MW | 46.8 kDa |
| Summary | Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_016720" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC209872 | Neu3 (untagged) - Mouse neuraminidase 3 (Neu3), (10ug) | 10 ug |
$457.00
|
|
| MG223297 | Neu3 (tGFP-tagged) - Mouse neuraminidase 3 (Neu3), (10ug) | 10 ug |
$886.00
|
|
| MR223297L3 | Lenti ORF clone of Neu3 (Myc-DDK-tagged) - Mouse neuraminidase 3 (Neu3) | 10 ug |
$986.00
|
|
| MR223297L4 | Lenti ORF clone of Neu3 (mGFP-tagged) - Mouse neuraminidase 3 (Neu3) | 10 ug |
$986.00
|
|
Citations
The sialidase NEU3 promotes pulmonary fibrosis in mice
Pilling, Sahlberg, Karhadkar et al
Respir Res (2022) 23 (1), 215 DOI: 10.1186/s12931-022-02146-y
Pilling, Sahlberg, Karhadkar et al
Respir Res (2022) 23 (1), 215 DOI: 10.1186/s12931-022-02146-y
Cited by 18
AI Summary: OriGene’s murine NEU3 expression plasmid (Cat# MR223297) was used to transfect cells for the production of recombinant NEU3 protein, enabling the study of NEU3’s role in promoting pulmonary fibrosis in mice.
Snippet: red in CDM4CHO medium with l-glutamine (Cat# SH30557.02, Hyclone/Cytiva, Marlborough, MA, USA). Cells (1 × 105) were mixed with 2 μg of 100 μg/ml of murine NEU3 expression plasmid (MR223297;Origene, Rockville, MD, USA) in 100 μl PBS (GE Lifesciences, Marlborough, MA, USA) and were transfected by electroporation using a 4D-Nucleofector System (Lonza, Walkersville, MD, USA) following the;2 μg of 100 μg/ml of murine NEU3 expression plasmid (MR223297;Origene, Rockville, MD) in 100 μl PBS (GE Lifesciences, Marlborough, MA) and were transfected by electroporation using a 4D-Nucleofector System (Lonza, Walkersville, MD) following the manufacturer’s
Showing 1-1 of 1 papers.
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