OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Bak1 (NM_007523) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR223116
Bak1 (Myc-DDK-tagged) - Mouse BCL2-antagonist/killer 1 (Bak1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Bak1 or NM_007523"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bak1
Synonyms Bak; N-Bak; N-BAK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223116 representing NM_007523
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCTGGACAAGGACCAGGTCCCCCGAAGGTGGGCTGCGATGAGTCCCCGTCCCCTTCTGAACAGC
AGGTTGCCCAGGACACAGAGGAGGTCTTTCGAAGCTACGTTTTTTACCTCCACCAGCAGGAACAGGAGAC
CCAGGGGGCGGCCGCCCCTGCCAACCCCGAGATGGACAACTTGCCCCTGGAACCCAACAGCATCTTGGGT
CAGGTGGGTCGGCAGCTTGCTCTCATCGGAGATGATATTAACCGGCGCTACGACACAGAGTTCCAGAATT
TACTAGAACAGCTTCAGCCCACAGCCGGGAATGCCTACGAACTCTTCACCAAGATCGCCTCCAGCCTATT
TAAGAGTGGCATCAGCTGGGGCCGCGTGGTGGCTCTCCTGGGCTTTGGCTACCGTCTGGCCCTGTACGTC
TACCAGCGTGGTTTGACCGGCTTCCTGGGCCAGGTGACCTGCTTTTTGGCTGATATCATACTGCATCATT
ACATCGCCAGATGGATCGCACAGAGAGGCGGTTGGGTGGCAGCCCTGAATTTTCGTAGAGACCCCATCCT
GACCGTAATGGTGATTTTTGGTGTGGTTCTGTTGGGCCAATTCGTGGTACACAGATTCTTCAGATCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223116 representing NM_007523
Red=Cloning site Green=Tags(s)

MASGQGPGPPKVGCDESPSPSEQQVAQDTEEVFRSYVFYLHQQEQETQGAAAPANPEMDNLPLEPNSILG
QVGRQLALIGDDINRRYDTEFQNLLEQLQPTAGNAYELFTKIASSLFKSGISWGRVVALLGFGYRLALYV
YQRGLTGFLGQVTCFLADIILHHYIARWIAQRGGWVAALNFRRDPILTVMVIFGVVLLGQFVVHRFFRS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007523
ORF Size 627 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007523.3
RefSeq Size 2017 bp
RefSeq ORF 630 bp
Locus ID 12018
UniProt ID O08734
Cytogenetics 17 A3.3
MW 23.7 kDa
Summary In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_007523" in other vectors (4)
SKU Description Size Price
MC208173 Bak1 (untagged) - Mouse BCL2-antagonist/killer 1 (Bak1), (10ug) 10 ug
$330.00
MG223116 Bak1 (tGFP-tagged) - Mouse BCL2-antagonist/killer 1 (Bak1), (10ug) 10 ug
$489.00 $500.00
MR223116L3 Lenti ORF clone of Bak1 (Myc-DDK-tagged) - Mouse BCL2-antagonist/killer 1 (Bak1) 10 ug
$600.00
MR223116L4 Lenti ORF clone of Bak1 (mGFP-tagged) - Mouse BCL2-antagonist/killer 1 (Bak1) 10 ug
$600.00

Plasmid Map for MR223116

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223116
Circular map for MR223116

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products