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Col9a1 (NM_007740) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223039
Col9a1 (Myc-DDK-tagged) - Mouse collagen, type IX, alpha 1 (Col9a1)
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ORF Plasmid:
DDK tGFP
$589.00 $843.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Col9a1
Synonyms Col9a-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223039 representing NM_007740
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAACTTCTGGAAAATCTCAGTTTTCTTCTGTGTGTGCAGTTGTCTGGGACCCTGGGTATCCGCAA
CTCTTAAGCGTCGTGCAAGATTTCCTGCCAATTCCATTTCTAATGGTGGAAGTGAACTGTGTCCCAAGAT
CAGGATTGGCCAAGATGACTTACCAGGGTTTGACCTGATTTCTCAGTTCCAAATTGAGAAGGCTGCATCT
CGAAGGACTATCCAGAGGGTGGTGGGATCCACAGCCTTACAAGTGGCTTACAAGCTGGGAAGTAATGTAG
ACTTCAGGATTCCAACAAGGCATTTGTATCCCAGTGGACTGCCTGAAGAATATTCCTTTTTAACTACTTT
CCGGATGACTGGAAGCACACTTGAAAAGCACTGGAACATTTGGCAGATCCAGGATTCCGCAGGGAGAGAG
CAAGTTGGCGTGAAGATAAATGGCCAAACAAAGTCTGTCGCATTTTCATACAAGGGACTGGATGGGAGTC
TCCAAACGGCTGCCTTCTTGAATTTGCCGTCCTTGTTTGACTCCCGGTGGCATAAGCTCATGATTGGCGT
GGAAAGAACAAGCGCCACTCTTTTTATTGACTGCATCAGGATCGAATCTTTACCTATAAAGCCAAGAGGC
CAGATTGATGCGGATGGCTTTGCAGTGCTGGGAAAACTTGTGGACAATCCTCAGGTTTCTGTTCCTTTTG
AACTCCAGTGGATGCTGATTCATTGTGACCCCCTGAGACCCAGGAGAGAAACCTGTCATGAGCTGCCAAT
CAGAATCACAACCAGCCAGACCACTGATGAGAGAGGTCCTCCGGGTGAGCAGGGGCCTCCAGGGCCTCCT
GGGCCTCCTGGAGTTCCGGGCATAGATGGCATTGATGGTGACCGAGGTCCAAAGGGTCCCCCAGGACCTC
CGGGTCCTCCTGGAGACCCAGGCAAGCCAGGAGCACCAGGCAAGCCAGGCACACCAGGAGCTGATGGATT
AACAGGACCTGATGGATCCCCTGGCTCTGTTGGACCAAGGGGACAAAAAGGAGAACCTGGTGTACCTGGG
TCTCGTGGATTTCCAGGCCGTGGTATTCCAGGACCCCCTGGTCCTCCTGGGACCACAGGACTTCCTGGAG
AACTTGGCCGAGTAGGCCCTATTGGAGACCCTGGGAAAAGAGGACCACCTGGCCCTCCTGGACCTCCAGG
ACCCAGTGGAACAATTGGATTTCATGATGGAGACCCATTGTGCCCCAATTCCTGCCCACCAGGTCGCTCT
GGATATCCAGGCCTACCAGGCATGAGGGGCCATAAAGGGGCGAAAGGAGAAATCGGCGAGCCAGGAAGAC
AAGGACACAAGGGTGAAGAGGGTGACCAGGGGGAACTGGGAGAAGTTGGCGATCAAGGACCTCCAGGACC
TCAGGGTCTGAGAGGCATCACTGGCATAGTTGGAGACAAAGGAGAAAAGGGTGCTCGGGGATTTGATGGA
GAGCCTGGACCTCAGGGCATTCCAGGTGCAGCTGGTGATCAAGGACAGCGAGGCCCTCCAGGAGAAACAG
GTCCTGAGGGAGACAGAGGCATTCAAGGTTCCCGAGGAATTCCTGGATCCCCAGGGCCAAAAGGAGACAC
GGGCTTGCCAGGTGTAGATGGCCGAGATGGAATACCAGGAATGCCCGGGACAAAGGGTGAAGCAGGGAAG
CCTGGACCCCCCGGGGACGTGGGATTGCAGGGCTTACCAGGTGTCCCTGGAATCCCTGGTGCAAAAGGTG
TTGCCGGTGAAAAGGGTAACACGGGTGCTCCAGGAAAGCCCGGTCAGTTGGGAAGTTCAGGGAAACCAGG
CCAACAAGGGCCCCCAGGAGAGGTTGGACCTCGGGGACCCAGGGGCCTTCCAGGCAGTAGAGGCCCGGTA
GGACCAGAAGGGTCTCCAGGCATACCAGGGAAACTGGGATCTGTTGGCAGCCCTGGCCTTCCTGGCTTGC
CTGGGCCCCCTGGACTTCCTGGAATGAAAGGAGACAGGGGTGTATTTGGTGAACCGGGTCCCAAGGGTGA
ACAGGGTGCCTCTGGTGAAGAAGGTGAAGCAGGAGCAAGGGGTGACCTTGGAGATATGGGACAACCTGGC
CCAAAGGGATCTGTGGGTAACCCCGGGGAGCCGGGTCTGAGGGGGCCTGAAGGAATCAGAGGGCTTCCTG
GAGTGGAAGGACCAAGAGGACCACCTGGACCCCGGGGCATGCAGGGAGATCAGGGTGCCACTGGGCTGCC
TGGTATCCAGGGCCCTCCGGGCAGAGCGCCGACCGACCAGCACATCAAGCAGGTTTGCATGAGAGTCGTG
CAAGAGCATTTTGCGGAAATGGCAGCCAGCCTCAAGAGACCAGACACAGGAGCCTCTGGTCTTCCTGGGA
GGCCTGGCCCCCCTGGGCCTCCGGGCCCCCCTGGAGAGAATGGTTTCCCTGGTCAGATGGGAATCCGTGG
TCTCCCAGGCATTAAGGGTCCCCCTGGTGCTCTTGGCTTAAGAGGACCTAAAGGAGACTTGGGAGAAAAA
GGAGAACGTGGTCCTCCAGGAAGAGGTCCTAAGGGTTTGCCTGGAGCGATAGGTCTCCCAGGTGACCCAG
GCCCTGCCAGCTATGGGAAAAATGGCCGTGACGGAGAGCAAGGTCCCCCGGGAGTGGCAGGAATTCCTGG
TGTGCCTGGACCCCCAGGTCCTCCAGGCCCTCCTGGGTTCTGTGAGCCAGCCTCTTGCACCCTGCAGTCT
GGTCAAAGAGCATTTAGCAAAGGGCCGGACAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223039 representing NM_007740
Red=Cloning site Green=Tags(s)

MKNFWKISVFFCVCSCLGPWVSATLKRRARFPANSISNGGSELCPKIRIGQDDLPGFDLISQFQIEKAAS
RRTIQRVVGSTALQVAYKLGSNVDFRIPTRHLYPSGLPEEYSFLTTFRMTGSTLEKHWNIWQIQDSAGRE
QVGVKINGQTKSVAFSYKGLDGSLQTAAFLNLPSLFDSRWHKLMIGVERTSATLFIDCIRIESLPIKPRG
QIDADGFAVLGKLVDNPQVSVPFELQWMLIHCDPLRPRRETCHELPIRITTSQTTDERGPPGEQGPPGPP
GPPGVPGIDGIDGDRGPKGPPGPPGPPGDPGKPGAPGKPGTPGADGLTGPDGSPGSVGPRGQKGEPGVPG
SRGFPGRGIPGPPGPPGTTGLPGELGRVGPIGDPGKRGPPGPPGPPGPSGTIGFHDGDPLCPNSCPPGRS
GYPGLPGMRGHKGAKGEIGEPGRQGHKGEEGDQGELGEVGDQGPPGPQGLRGITGIVGDKGEKGARGFDG
EPGPQGIPGAAGDQGQRGPPGETGPEGDRGIQGSRGIPGSPGPKGDTGLPGVDGRDGIPGMPGTKGEAGK
PGPPGDVGLQGLPGVPGIPGAKGVAGEKGNTGAPGKPGQLGSSGKPGQQGPPGEVGPRGPRGLPGSRGPV
GPEGSPGIPGKLGSVGSPGLPGLPGPPGLPGMKGDRGVFGEPGPKGEQGASGEEGEAGARGDLGDMGQPG
PKGSVGNPGEPGLRGPEGIRGLPGVEGPRGPPGPRGMQGDQGATGLPGIQGPPGRAPTDQHIKQVCMRVV
QEHFAEMAASLKRPDTGASGLPGRPGPPGPPGPPGENGFPGQMGIRGLPGIKGPPGALGLRGPKGDLGEK
GERGPPGRGPKGLPGAIGLPGDPGPASYGKNGRDGEQGPPGVAGIPGVPGPPGPPGPPGFCEPASCTLQS
GQRAFSKGPDK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007740
ORF Size 2763 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007740.3, NP_031766.3
RefSeq Size 3888 bp
RefSeq ORF 2766 bp
Locus ID 12839
UniProt ID Q05722
Cytogenetics 1 9.95 cM
MW 92.1 kDa
Summary Structural component of hyaline cartilage and vitreous of the eye.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007740" in other vectors (2)
SKU Description Size Price
MC222624 Col9a1 (untagged) - Mouse collagen, type IX, alpha 1 (Col9a1), (10ug) 10 ug
$928.00
MG223039 Col9a1 (tGFP-tagged) - Mouse collagen type IX alpha 1 (Col9a1), (10ug) 10 ug
$1,043.00

Plasmid Map for MR223039

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223039
Circular map for MR223039

file-alt Citations

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