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Lyar (NM_025281) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222894
Lyar (Myc-DDK-tagged) - Mouse Ly1 antibody reactive clone (Lyar)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Lyar or NM_025281"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lyar
Synonyms MLZ-264
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222894 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTATTTTTTACATGCAATGCATGTGGTGAATCCGTGAAGAAAATACAAGTGGAAAAGCATGTGTCTA
ACTGCAGAAACTGTGAATGTCTTTCCTGCATTGACTGTGGGAAAGATTTCTGGGGTGATGACTACAAAAG
CCATGTGAAGTGCATCAGTGAAGGTCAGAAGTACGGAGGCAAAGGCTATGAAGCCAAGACACACAAAGGT
GATGCAAAACAGCAGGCATGGATTCAGAAAATTAATGAGTTAATAAAGAAACCCAACGTCAGCCCCAAGG
TGCGAGAACTTTTGCAGCAAATCAGTGCTTTTGATAATGTTCCCAGAAAAAAGGCGAAGTTTCAGAACTG
GATGAAAAACAGCCTGAAAGTGCACAGCGACTCCGTTCTAGAGCAGGTGTGGGATATCTTCTCCGAAGCA
TCCAGCAGTGAGCAAGATCAGCAGCAGCCACCCAGCCACACGGCCAAGCCACATGCAGAGATGCCGATCA
CTAAAGTTCCATCTGCAAAAACAAATGGCACCACAGAAGAGCAAACCGAGGCGAAGAAGAATAAAAGAGA
AAGGAAGGAGGAACGGCAGAAAAACAGGAAGAAAGAGAAGAAAGAGCTGAAGCTAGAAAACCACCAGGAA
AACCTGAGGGGCCAGAAGCCAAAGAAGCGCAAGAAGAACCAGGAGGCCGGACATGAGGCTGCTGGGGAGG
AGGCCGCCGAGGCCAGCGGCCCACCGGAGAAGAAGAAAGCCCAGGGAGGACAGGCTTCTGAAGAGGGAGC
AGACAGAAATGGGGGCCCTGGGGAGGATGCTGCCGAGGGGCAGACCAAGACAGCAGCAGGGAAACGGAAG
CGGCCAAAGCACTCAGGAGCTGAGTCTGGTTACAAGAAAAAAAAGATGAAGTTACCAGAGCAGCCTGAAG
AGGGAGAGGCCAAGGACCACGAGGCTCCATCGAAAGGTAAATTCAACTGGAAGGGGACCATTAAGGCTGT
TTTGAAACAGGCTCCAGACAACGAGATATCAGTCAAGAAGTTAAAGAAAAAGGTTATAGCTCAGTACCAT
GCGGTGATGAACGACCATCACACATCGGAGGAGGAGCTCCTGGCCATCTTCAACAGGAAGATCAGCAGGA
ACCCCACCTTTAAGGTGCTGAAGGACAGAGTAAAGCTTCTGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222894 protein sequence
Red=Cloning site Green=Tags(s)

MVFFTCNACGESVKKIQVEKHVSNCRNCECLSCIDCGKDFWGDDYKSHVKCISEGQKYGGKGYEAKTHKG
DAKQQAWIQKINELIKKPNVSPKVRELLQQISAFDNVPRKKAKFQNWMKNSLKVHSDSVLEQVWDIFSEA
SSSEQDQQQPPSHTAKPHAEMPITKVPSAKTNGTTEEQTEAKKNKRERKEERQKNRKKEKKELKLENHQE
NLRGQKPKKRKKNQEAGHEAAGEEAAEASGPPEKKKAQGGQASEEGADRNGGPGEDAAEGQTKTAAGKRK
RPKHSGAESGYKKKKMKLPEQPEEGEAKDHEAPSKGKFNWKGTIKAVLKQAPDNEISVKKLKKKVIAQYH
AVMNDHHTSEEELLAIFNRKISRNPTFKVLKDRVKLLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025281
ORF Size 1164 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025281.3, NP_079557.2
RefSeq Size 1568 bp
RefSeq ORF 1167 bp
Locus ID 17089
UniProt ID Q08288
Cytogenetics 5 20.29 cM
MW 43.7 kDa
Summary Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (By similarity). Also acts at the level of transcription regulation. Along with PRMT5, binds embryonic globin promoter (By similarity). Represses the expression of embryonic globin Hbb-y gene (PubMed:25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and that encoding the small nucleolar RNA SNORD41 (By similarity). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (By similarity). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (PubMed:25735755). Prevents NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (PubMed:19489080).UniProtKB/Swiss-Prot Function
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"NM_025281" in other vectors (4)
SKU Description Size Price
MC208919 Lyar (untagged) - Mouse Ly1 antibody reactive clone (Lyar), (10ug) 10 ug
$503.00
MG222894 Lyar (tGFP-tagged) - Mouse Ly1 antibody reactive clone (Lyar), (10ug) 10 ug
$657.00
MR222894L3 Lenti ORF clone of Lyar (Myc-DDK-tagged) - Mouse Ly1 antibody reactive clone (Lyar) 10 ug
$757.00
MR222894L4 Lenti ORF clone of Lyar (mGFP-tagged) - Mouse Ly1 antibody reactive clone (Lyar) 10 ug
$757.00

Plasmid Map for MR222894

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222894
Circular map for MR222894

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