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Hesx1 (NM_010420) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222822
Hesx1 (Myc-DDK-tagged) - Mouse homeobox gene expressed in ES cells (Hesx1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Hesx1 or NM_010420"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hesx1
Synonyms HES-1; Rpx
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222822 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCCCAGCCTTCGGGAAGGTGCTCAGCTCCGGGAAAGCAAGCCCGCGCCCTGCTCCTTCTCAATTG
AGAGCATTTTAGGACTGGACCAGAAAAAAGATTGTACAACGTCAGTAAGACCCCACAGACCCTGGACAGA
CACCTGCGGTGACTCAGAGAAAGGTGGCAACCCACCCCTACATGCCCCAGATCTTCCCAGTGAGACTTCA
TTTCCTTGTCCAGTGGATCATCCAAGGCCAGAAGAAAGGGCTCCGAAATATGAAAATTATTTTTCAGCCT
CCGAAACACGCTCTTTGAAAAGAGAATTGAGTTGGTACCGAGGACGAAGGCCAAGAACCGCTTTCACCCA
GAACCAGGTCGAAGTATTGGAAAATGTCTTTAGAGTGAACTGCTACCCTGGCATTGACATCAGAGAGGAC
CTAGCTCAAAAGCTGAACTTAGAGGAAGACAGAATCCAGATTTGGTTCCAAAATCGCCGAGCAAAGATGA
AAAGGTCCCGTAGAGAATCACAGTTTCTAATGGCGAAAAAACCCTTCAACCCAGATCTTCTGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222822 protein sequence
Red=Cloning site Green=Tags(s)

MSPSLREGAQLRESKPAPCSFSIESILGLDQKKDCTTSVRPHRPWTDTCGDSEKGGNPPLHAPDLPSETS
FPCPVDHPRPEERAPKYENYFSASETRSLKRELSWYRGRRPRTAFTQNQVEVLENVFRVNCYPGIDIRED
LAQKLNLEEDRIQIWFQNRRAKMKRSRRESQFLMAKKPFNPDLLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010420
ORF Size 555 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010420.2, NP_034550.2
RefSeq Size 1144 bp
RefSeq ORF 558 bp
Locus ID 15209
UniProt ID Q61658
Cytogenetics 14 A3
MW 21.5 kDa
Summary Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation.UniProtKB/Swiss-Prot Function
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"NM_010420" in other vectors (4)
SKU Description Size Price
MC208660 Hesx1 (untagged) - Mouse homeobox gene expressed in ES cells (Hesx1), (10ug) 10 ug
$330.00
MG222822 Hesx1 (tGFP-tagged) - Mouse homeobox gene expressed in ES cells (Hesx1), (10ug) 10 ug
$500.00
MR222822L3 Lenti ORF clone of Hesx1 (Myc-DDK-tagged) - Mouse homeobox gene expressed in ES cells (Hesx1) 10 ug
$600.00
MR222822L4 Lenti ORF clone of Hesx1 (mGFP-tagged) - Mouse homeobox gene expressed in ES cells (Hesx1) 10 ug
$600.00

Plasmid Map for MR222822

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222822
Circular map for MR222822

file-alt Citations

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