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Cacna2d1 (NM_001110843) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222813
Cacna2d1 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant a
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $960.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cacna2d1
Synonyms Ca(v)alpha2delta1; Cac; Cacna2; Cchl; Cchl2a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222813 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTGGCTGCCTGCTGGCCTTGACTCTGACACTTTTCCAATCTGGGCTGATCGGCCCCTCGAGCG
AGGAGCCCTTCCCTTCGCCCGTCACTATCAAGTCATGGGTGGACAAGATGCAAGAAGACCTTGTCACACT
GGCAAAAACAGCAAGTGGAGTGACTCAGCTTGCTGATATTTATGAAAAATACCAAGATTTGTATACTGTG
GAGCCCAACAATGCACGCCAACTGGTTGAAATTGCAGCCCGAGACATTGAGAAGCTTCTAAGCAACAGAT
CTAAGGCCCTGGTGCGCCTGGCTATGGAAGCAGAGAAAGTCCAAGCAGCCCACCAATGGAGGGAAGATTT
TGCAAGCAATGAAGTTGTCTACTATAATGCTAAGGATGATCTTGATCCTGAAAGAAATGAGAGTGAGCCA
GGCAGCCAACGGATTAAACCTGTTTTTATTGAAGATGCTAATTTTGGACGTCAGATATCCTATCAGCATG
CAGCGGTCCATATTCCCACGGACATCTATGAGGGCTCAACCATAGTGTTAAACGAACTCAACTGGACAAG
TGCCTTAGATGAAGTATTCAAAAGAAATCGAGACGAAGACCCTACACTGCTGTGGCAAGTGTTTGGCAGC
GCCACTGGCCTGGCCCGATATTATCCAGCTTCTCCATGGGTGGATAATAGTAGAACTCCAAACAAGATTG
ATCTATATGATGTACGCAGAAGACCATGGTACATCCAGGGAGCTGCATCCCCCAAGGACATGCTCATTCT
GGTGGACGTGAGTGGAAGTGTGAGCGGATTGACTCTGAAACTCATCCGAACATCTGTCTCCGAGATGTTA
GAAACCCTCTCTGATGATGATTTCGTGAATGTAGCTTCATTTAACAGCAACGCTCAGGATGTAAGCTGTT
TCCAGCACCTGGTTCAAGCGAATGTAAGAAATAAGAAGGTGTTGAAAGATGCCGTGAATAACATTACAGC
AAAGGGGATCACAGATTACAAGAAAGGCTTTAGCTTTGCCTTCGAACAGCTACTTAATTATAATGTTTCC
AGAGCTAATTGCAATAAGATTATCATGTTATTCACGGATGGAGGAGAAGAGAGAGCCCAGGAGATATTTG
CCAAATACAATAAAGACAAAAAAGTCCGTGTGTTTACATTTTCCGTCGGTCAACATAATTATGACAGAGG
ACCTATTCAGTGGATGGCTTGTGAAAATAAAGGTTACTATTATGAGATTCCCTCCATTGGTGCAATAAGA
ATCAATACTCAGGAATATCTAGATGTTCTGGGAAGACCAATGGTTTTAGCTGGTGACAAAGCGAAGCAAG
TTCAATGGACAAATGTGTATTTGGACGCCCTGGAACTGGGACTTGTCATTACTGGAACTCTACCAGTCTT
CAACGTCACTGGCCAATCTGAAAATAAGACAAACTTGAAGAACCAGTTGATTCTTGGTGTGATGGGCGTT
GATGTGTCTCTGGAAGATATCAAGAGATTGACACCACGTTTTACACTCTGTCCCAATGGCTACTATTTTG
CAATTGATCCTAATGGCTATGTCTTATTGCATCCAAATCTTCAGCCAAAGCCTATTGGTGTAGGTATACC
GACAATTAATTTAAGGAAAAGGAGACCCAACGTTCAGAACCCCAAATCTCAGGAGCCAGTCACACTGGAT
TTTCTCGATGCTGAGTTAGAGAATGAAATTAAAGTGGAGATTCGAAATAAAATGATAGATGGAGAAAGTG
GAGAAAAAACGTTCAGAACTCTGGTCAAGTCTCAAGATGAGAGATACATTGACAAAGGAAATCGAACATA
CACATGGACGCCTGTCAATGGCACAGATTACAGTTTGGCCTTGGTATTGCCAACCTACAGTTTTTACTAT
ATAAAAGCCAAACTAGAAGAGACAATAACTCAGGCCAGATATTCAGAAACCCTGAAGCCAGACAATTTTG
AAGAATCTGGCTATACTTTCATAGCACCAAGGGAATACTGCAATGATCTTAAACCTTCAGATAATAACAC
TGAATTTCTTTTGAATTTCAATGAATTTATTGATAGGAAAACTCCAAACAACCCCTCCTGTAATACAGAT
TTGATTAATAGAATCTTGCTGGATGCAGGTTTTACAAATGAACTTGTCCAAAATTATTGGAGTAAGCAGA
AAAATATCAAAGGAGTGAAGGCACGCTTTGTGGTGACAGACGGTGGGATTACGAGAGTTTATCCCAAAGA
GGCCGGAGAAAATTGGCAAGAGAACCCAGAGACGTACGAGGACAGCTTCTACAAACGGAGCCTAGATAAC
GATAACTACGTTTTCACTGCGCCCTACTTTAACAAAAGTGGACCTGGTGCGTATGAATCTGGAATTATGG
TAAGCAAAGCAGTAGAACTGTATATCCAAGGAAAACTTCTTAAGCCCGCAGTTGTGGGAATTAAAATTGA
TGTAAATTCTTGGATAGAAAATTTTACCAAAACTTCAATCAGGGATCCGTGTGCTGGTCCAGTTTGTGAC
TGCAAAAGAAACAGTGATGTAATGGACTGTGTCATTCTAGATGATGGTGGATTTCTTTTGATGGCAAATC
ACGATGATTACACTAATCAGATTGGACGTTTTTTTGGAGAGATTGACCCAAGCATGATGAGACACCTGGT
TAATATATCACTTTATGCATTCAACAAATCATATGACTATCAGTCCGTGTGCGATCCAGGGGCAGCACCA
AAGCAGGGGGCAGGACATCGCTCAGCATATGTGCCATCGATTGCAGATATACTGCAGATTGGCTGGTGGG
CCACCGCTGCCGCCTGGTCTATTCTCCAGCAGCTGCTCTTGAGTTTGACATTTCCACGGCTCCTTGAGGC
AGTTGAGATGGAGGAAGATGACTTCACAGCCTCCCTGTCTAAGCAGAGCTGCATCACAGAACAAACCCAG
TACTTCTTCAAGAACGATACTAAATCATTCAGTGGTTTACTGGACTGTGGAAACTGTTCCAGGATCTTTC
ATGTAGAGAAACTTATGAACACCAACTTAGTATTCATAATGGTGGAGAGCAAAGGGACATGTCCGTGTGA
CACGCGGCTGCTCATGCAAGCGGAACAGACTTCTGATGGTCCAGATCCTTGCGACATGGTCAAGCAGCCC
AGATACCGAAAAGGACCTGATGTCTGCTTTGATAATAATGTGCTGGAGGATTATACTGACTGTGGTGGTG
TTTCTGGGTTAAACCCTTCCTTATGGTCTATCTTTGGACTCCAGTTTATACTCCTTTGGCTGGTATCTGG
CAGCAGACACTACCTACTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222813 protein sequence
Red=Cloning site Green=Tags(s)

MAAGCLLALTLTLFQSGLIGPSSEEPFPSPVTIKSWVDKMQEDLVTLAKTASGVTQLADIYEKYQDLYTV
EPNNARQLVEIAARDIEKLLSNRSKALVRLAMEAEKVQAAHQWREDFASNEVVYYNAKDDLDPERNESEP
GSQRIKPVFIEDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKRNRDEDPTLLWQVFGS
ATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEML
ETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVS
RANCNKIIMLFTDGGEERAQEIFAKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIR
INTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNVTGQSENKTNLKNQLILGVMGV
DVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGVGIPTINLRKRRPNVQNPKSQEPVTLD
FLDAELENEIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYY
IKAKLEETITQARYSETLKPDNFEESGYTFIAPREYCNDLKPSDNNTEFLLNFNEFIDRKTPNNPSCNTD
LINRILLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDN
DNYVFTAPYFNKSGPGAYESGIMVSKAVELYIQGKLLKPAVVGIKIDVNSWIENFTKTSIRDPCAGPVCD
CKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSMMRHLVNISLYAFNKSYDYQSVCDPGAAP
KQGAGHRSAYVPSIADILQIGWWATAAAWSILQQLLLSLTFPRLLEAVEMEEDDFTASLSKQSCITEQTQ
YFFKNDTKSFSGLLDCGNCSRIFHVEKLMNTNLVFIMVESKGTCPCDTRLLMQAEQTSDGPDPCDMVKQP
RYRKGPDVCFDNNVLEDYTDCGGVSGLNPSLWSIFGLQFILLWLVSGSRHYLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001110843
ORF Size 3309 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001110843.1, NP_001104313.1
RefSeq Size 7472 bp
RefSeq ORF 3312 bp
Locus ID 12293
UniProt ID O08532
Cytogenetics 5 6.56 cM
MW 124.6 kDa
Summary This gene encodes a regulatory component of the voltage-dependent calcium channel complex. The product of this gene is a proprotein that is proteolytically processed into alpha-2 and delta subunits, which are linked by a disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Jan 2013
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Documents
Resources
"NM_001110843" in other vectors (4)
SKU Description Size Price
MC223541 Cacna2d1 (untagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant a, (10ug) 10 ug
$1,057.00
MG222813 Cacna2d1 (tGFP-tagged) - Mouse calcium channel voltage-dependent alpha2/delta subunit 1 (Cacna2d1) transcript variant a, (10ug) 10 ug
$1,160.00
MR222813L3 Lenti ORF clone of Cacna2d1 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant a 10 ug
$1,260.00
MR222813L4 Lenti ORF clone of Cacna2d1 (mGFP-tagged) - Mouse calcium channel, voltage-dependent, alpha2/delta subunit 1 (Cacna2d1), transcript variant a 10 ug
$1,260.00

Plasmid Map for MR222813

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222813
Circular map for MR222813

file-alt Citations

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