Dstn (NM_019771) Mouse Tagged ORF Clone
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Dstn |
| Synonyms | 2610043P17Rik; ADF; AU042046; corn1; Dsn; sid23p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_019771 |
| ORF Size | 495 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_019771.2, NP_062745.1 |
| RefSeq Size | 1918 bp |
| RefSeq ORF | 498 bp |
| Locus ID | 56431 |
| UniProt ID | Q9R0P5 |
| Cytogenetics | 2 70.89 cM |
| MW | 19 kDa |
| Summary | Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_019771" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC210056 | Dstn (untagged) - Mouse destrin (Dstn), (10ug) | 10 ug |
$240.00
|
|
| MG222274 | Dstn (tGFP-tagged) - Mouse destrin (Dstn), (10ug) | 10 ug |
$425.00
|
|
| MR222274L1 | Lenti ORF clone of Dstn (Myc-DDK-tagged) - Mouse destrin (Dstn) | 10 ug |
$525.00
|
|
| MR222274L2 | Lenti ORF clone of Dstn (mGFP-tagged) - Mouse destrin (Dstn) | 10 ug |
$525.00
|
|
| MR222274L3 | Lenti ORF clone of Dstn (Myc-DDK-tagged) - Mouse destrin (Dstn) | 10 ug |
$525.00
|
|
| MR222274L4 | Lenti ORF clone of Dstn (mGFP-tagged) - Mouse destrin (Dstn) | 10 ug |
$525.00
|
|
Citations
Integrative analysis of aging-related genes reveals CEBPA as a novel therapeutic target in non-small cell lung cancer
Zhu, Zhu, Shi et al
Cancer Cell Int (2024) 24 (1), 267 DOI: 10.1186/s12935-024-03457-4
Zhu, Zhu, Shi et al
Cancer Cell Int (2024) 24 (1), 267 DOI: 10.1186/s12935-024-03457-4
Cited by 7
AI Summary: OriGene provided the CEBPA overexpression plasmid used in this study to investigate the effects of CEBPA on non-small cell lung cancer cell proliferation, migration, and invasion.
Snippet: The overexpression plasmid we used was from ORIGENE (Wuxi, China). The sequence data of CEBPA siRNA and overexpression plasmid are in Table S4
Identification of the growth cone as a probe and driver of neuronal migration in the injured brain
Nakajima, Sawada, Umeda et al
Nat Commun (2024) 15 (1), 1877 DOI: 10.1038/s41467-024-45825-8
Nakajima, Sawada, Umeda et al
Nat Commun (2024) 15 (1), 1877 DOI: 10.1038/s41467-024-45825-8
Cited by 26
AI Summary: In this study, OriGene’s Myc-DDK-tagged mouse ORF clones for Syntaxin-7 (Cat. MR221576) and Destrin (Cat. MR222274) were used to investigate the molecular mechanisms underlying growth cone dynamics and neuronal migration in the injured brain.
Snippet: ), pCAGGS-flag-Cortactin*-IRES-mCherry, and pCAGGS-flag-Cortactin*Y421A-IRES-mCherry]. pCAGGS-EmGFP-miR-fyn (Fyn-KD) was employed69. The plasmid of Myc-DDK-tagged mouse ORF clones were purchased from OriGene [Syntaxin-7 (Cat. MR221576) and Destrin (Cat. MR222274)]. Vector KD efficiency was assessed by western blotting and immunocytochemistry. Primary neuronal culture Cortical tissues dissected fr
Showing 1-2 of 2 papers.
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