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Ky (NM_024291) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222260
Ky (Myc-DDK-tagged) - Mouse kyphoscoliosis peptidase (Ky)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $615.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ky
Synonyms CD1; D9Mgc44e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222260 representing NM_024291
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGAAGAAGGACAGCAACGCTGTGGCCATCGACATGCTGCTCATCGTGCACTCCGAGAAGCGGC
GCGCGGCGCAAGCCACGCACTTGGACCCGCAGGCGAACCCGGGCGCGCTGCTGCAAAACCGAGGAGGATT
CCAAGGTGTCAGAAACGGAATCCGGAAGTGGCAAGAGTTGGAAGAAAACAGCTTTCAGGGAAACTTGCCA
GAGAAGCAGTGCCTTCAGCAGCCGCAGGTCATCACCTCCTACGACAACCAAGGTACTCAGCTGACTGTGG
AAATCCACCCCCAAGACGCCATGCCCCAGCTGCTTAAGAAGTTCTCTTTGGCTAAACGTTTACAAGGTGA
TAAAAATGGAAACATGAGACCCCGGCAGCCTGGAGGGAAAGATGCCCACGCCTACCCCTGGGACAGATCC
AGTTTGAAATCCATGCCCCTGGACCTCCGGCTCTTTGAAAAACTGGATGCCTCTGCCTCACAGGTGACAG
TCAAGAGTGGCCTGAATGAGCTGGTGAGTGACTTGCTCCAGGAAGCCCACAGCGATCTGGAAAGGGTCAG
AGCCATCTGGATCTGGATCTGCCACCATATAGAGTACGACGTTGAGGCTGCCCAGGAGAAGGACCGCCAG
GCCTTCAAACCCACTGACATCCTGAGGACCCAGAAGACCAACTGTGATGGTTACGCCGGCCTCTTTGAAA
GGATGTGCAGGGTTGCTGGTGTGCAGTGTGTCACCGTGCCTGGCTACTCCAAGGGCTTTGGCTACCAGAC
CGGCCAAAGCTTCTCCGGAGAGTTCGACCACGCCTGGAACGCTGTGTACCTTGAGGGTCGTTGGCACCTG
GTGGACAGCACCTGGGGCAGCGGCCTGGTGGACACCACCACCTCCAAATTCACCTTCCTCTACAATGAAT
TCTACTTCCTCACCCACCCCGCCCTGTTCATCGAGGATCACTTCCCAGACAACAAAAACTGGCAGCTGCT
GAAGCCGCCCCAGTCTCTGAGGCAGTTTGAGAACAGCATGTACCACAAGAGTGAATTCTACAACAAGGGC
ATGCTGAGCGCCCACCCGGAGACGTCCATGATCAGAACAGTCAATGGGAAAGCCACAATCACCATCGAGA
GCCGGGCCCCAACACTGTTCATGTTCATGCTCAATGGCAAGCAGGAGCACGGGCTCCTGAGCCTCAGGAA
GAACGGGATGAAGCTGGAGGTGTACCCTCCAACCATGGGCACCCACAAGCTGCAGATCTTCGCCAAGGGC
AACTCTGAAATCTACAGCTCGGTGCTAGAATACACACTCAAGTGCAACTATGTAGACTTCTCTGTCCAGC
TGCCCAGTGAGCTCCACCAGCCCGTGGGCCCCAGCTGGTTCTCGGAGCAGATGGGCATCACGAAGCCCTC
TCACTCTGACCCTATCATTCACACCAGCGATGGCCGCTGCGCCATCAGCTTTAGCGTGGAAGAGGGCGTC
AGTGTCCTGGCATCCCTTCATGGCGATGACGGCCCCATCACTGAGGAGACACAGCGGCGCTACATCTTCC
AGTTGAACCGAGGGAAGAGGACCGAGCTCAAAGTCCAGCTGCCCCATGCCGGCAAGTTTGCCCTTAAGAT
CTTTGTGAAGAAGAGGCAGGAACAAGGGAACTTCATCTTTGTCTTCAACTACCTCTTGTGCTGCGCCAAC
ACCAAGGTGAACTGGCCCATGTTCCCTGAGAGCTTTGGCAACTGGGGACAGGACAATGAACTGCTAGAGC
CTCTCTCCGGTGTGCTTCCCGCCAACCGCAACATAGCCTTCAAACTAAAGCTGCATGGTATCGCCAAAGC
TCTGGTGAAAGGACAGGACACGTGGCCCCTCACTCTTAACCCCGAGGGCTACTGGGAGGGCAGCTGCAAC
ACAGCTGGCTGCCAGGAAGTCTACGTCATGGTGCTAGAGAATGCTAACCACAACTTCTACAGCTACATCC
TGAAATACAAAGTGAATGACCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222260 representing NM_024291
Red=Cloning site Green=Tags(s)

MELKKDSNAVAIDMLLIVHSEKRRAAQATHLDPQANPGALLQNRGGFQGVRNGIRKWQELEENSFQGNLP
EKQCLQQPQVITSYDNQGTQLTVEIHPQDAMPQLLKKFSLAKRLQGDKNGNMRPRQPGGKDAHAYPWDRS
SLKSMPLDLRLFEKLDASASQVTVKSGLNELVSDLLQEAHSDLERVRAIWIWICHHIEYDVEAAQEKDRQ
AFKPTDILRTQKTNCDGYAGLFERMCRVAGVQCVTVPGYSKGFGYQTGQSFSGEFDHAWNAVYLEGRWHL
VDSTWGSGLVDTTTSKFTFLYNEFYFLTHPALFIEDHFPDNKNWQLLKPPQSLRQFENSMYHKSEFYNKG
MLSAHPETSMIRTVNGKATITIESRAPTLFMFMLNGKQEHGLLSLRKNGMKLEVYPPTMGTHKLQIFAKG
NSEIYSSVLEYTLKCNYVDFSVQLPSELHQPVGPSWFSEQMGITKPSHSDPIIHTSDGRCAISFSVEEGV
SVLASLHGDDGPITEETQRRYIFQLNRGKRTELKVQLPHAGKFALKIFVKKRQEQGNFIFVFNYLLCCAN
TKVNWPMFPESFGNWGQDNELLEPLSGVLPANRNIAFKLKLHGIAKALVKGQDTWPLTLNPEGYWEGSCN
TAGCQEVYVMVLENANHNFYSYILKYKVNDQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024291
ORF Size 1983 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024291.3, NP_077253.3
RefSeq Size 5455 bp
RefSeq ORF 1986 bp
Locus ID 16716
Cytogenetics 9 54.54 cM
MW 75.1 kDa
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Documents
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"NM_024291" in other vectors (4)
SKU Description Size Price
MC220157 Ky (untagged) - Mouse kyphoscoliosis peptidase (Ky), (10ug) 10 ug
$678.00
MG222260 Ky (tGFP-tagged) - Mouse kyphoscoliosis peptidase (Ky), (10ug) 10 ug
$489.00 $815.00
MR222260L3 Lenti ORF clone of Ky (Myc-DDK-tagged) - Mouse kyphoscoliosis peptidase (Ky) 10 ug
$915.00
MR222260L4 Lenti ORF clone of Ky (mGFP-tagged) - Mouse kyphoscoliosis peptidase (Ky) 10 ug
$915.00

Plasmid Map for MR222260

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222260
Circular map for MR222260

file-alt Citations

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