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Nsmf (NM_001039386) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221711
Nsmf (Myc-DDK-tagged) - Mouse nasal embryonic LHRH factor (Nelf), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $495.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Nsmf or NM_001039386"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nsmf
Synonyms Jacob; Nelf
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221711 representing NM_001039386
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGCCGCCGCCTCCAGGAGGAGGGCGCTGAGGAGCGAGGCCATGTCCTCGGTAGCGGCCAAAGTAA
GAGCAGCCCGAGCGTTTGGAGAGTACCTGTCCCAGAGTCACCCTGAGAACCGCAACGGTGCAGACCACCT
GCTGGCTGACGCCTACTCTGGCCACGACGGGTCCCCAGAGATGCAACCTGCACCCCAGAACAAGCGCCGC
CTCTCCCTCGTCTCCAATGGCCGATATGAGGGCAGCATCTCAGATGAGGCAGTCAGCGGGAAGCCGGCTA
TAGAGGGCCCCCAGCCCCACGTGTACACCATCTCTAGAGAGCCCGCCCTGCTACCTGGCTCTGAAGCTGA
AGCCATTGAGCTAGCAGTGGTAAAAGGGAGGAGACAGCGGGAACGGCACCCTCACCACCACAGCCAGCCC
CTGCGTGCCAGCCCAGGCAGCAGCCGGGAGGACATCAGCAGGCCCTGCCAAAGCTGGGCAGGAAGCCGCC
AGGGCTCCAAAGAGTGCCCAGGATGTGCCCAGCTGGTCCCTGGTCCCTCTTCTCGGGCCTTTGGGCTGGA
GCAGCCACCTCTACCTGAGGCTCCTGGCCGCCACAAGAAGCTGGAAAGGATGTATAGCGTTGATGGAGTG
TCTGATGATGTCCCCATCCGTACCTGGTTCCCCAAGGAAAACCTTTTCAGCTTCCAGACGGCAACCACAA
CTATGCAAGCCATCTCGGTATTCAGGGGCTACGCGGAGAGGAAGCGTCGGAAACGGGAGAATGATTCCGC
GTCTGTAATCCAGAGGAACTTCCGCAAACACCTGCGCATGGTTGGCAGTCGGCGGGTGAAGGCCCAGACG
TTCGCTGAGCGTCGCGAACGGAGCTTCAGCCGGTCCTGGAGCGACCCCACCCCTATGAAAGCCGACACTT
CCCACGACTCCCGAGACAGCAGTGACCTACAGAGCTCACACTGCACCCTGGATGAGGCTTGTGAGGACCT
GGACTGGGACACAGAGAAAGGTCTGGAGGCCATGGCCTGCAACACCGAGGGCTTCTTGCCACCCAAGGTC
ATGCTCATCTCCTCCAAGGTGCCAAAGGCCGAGTATATCCCTACTATCATCCGCAGAGATGACCCGTCCA
TCATTCCCATCCTCTACGACCACGAGCATGCAACCTTCGAGGACATCCTGGAGGAAATAGAGAAGAAATT
GAACATCTATCACAAGGGGGCCAAGATCTGGAAGATGCTGATTTTCTGCCAGGGCGGCCCTGGACACCTT
TATTTGCTCAAGAACAAGGTGGCCACCTTTGCCAAAGTGGAGAAGGAAGAGGACATGATCCACTTCTGGA
AGAGGCTGAGCCGCCTGATGAGCAAGGTGAACCCCGAGCCGAATGTCATCCACATCATGGGCTGCTACAT
TCTGGGGAACCCTAACGGGGAGAAGCTATTCCAGAACCTCAGGACCCTCATGACCCCTTACAAGGTTACC
TTTGAGTCGCCTCTCGAGCTGTCTGCCCAAGGGAAGCAGATGATTGAGACCTACTTTGACTTCCGGCTGT
ACCGCCTGTGGAAGAGCCGCCAGCACTCAAAGCTGCTGGACTTTGATGACGTCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221711 representing NM_001039386
Red=Cloning site Green=Tags(s)

MGAAASRRRALRSEAMSSVAAKVRAARAFGEYLSQSHPENRNGADHLLADAYSGHDGSPEMQPAPQNKRR
LSLVSNGRYEGSISDEAVSGKPAIEGPQPHVYTISREPALLPGSEAEAIELAVVKGRRQRERHPHHHSQP
LRASPGSSREDISRPCQSWAGSRQGSKECPGCAQLVPGPSSRAFGLEQPPLPEAPGRHKKLERMYSVDGV
SDDVPIRTWFPKENLFSFQTATTTMQAISVFRGYAERKRRKRENDSASVIQRNFRKHLRMVGSRRVKAQT
FAERRERSFSRSWSDPTPMKADTSHDSRDSSDLQSSHCTLDEACEDLDWDTEKGLEAMACNTEGFLPPKV
MLISSKVPKAEYIPTIIRRDDPSIIPILYDHEHATFEDILEEIEKKLNIYHKGAKIWKMLIFCQGGPGHL
YLLKNKVATFAKVEKEEDMIHFWKRLSRLMSKVNPEPNVIHIMGCYILGNPNGEKLFQNLRTLMTPYKVT
FESPLELSAQGKQMIETYFDFRLYRLWKSRQHSKLLDFDDVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039386
ORF Size 1596 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039386.1, NP_001034475.1
RefSeq Size 2949 bp
RefSeq ORF 1599 bp
Locus ID 56876
UniProt ID Q99NF2
Cytogenetics 2 A3
MW 60.3 kDa
Summary Couples NMDA-sensitive glutamate receptor signaling to the nucleus and triggers long-lasting changes in the cytoarchitecture of dendrites and spine synapse processes. Part of the cAMP response element-binding protein (CREB) shut-off signaling pathway. Stimulates outgrowth of olfactory axons and migration of gonadotropin-releasing hormone (GnRH) and luteinizing-hormone-releasing hormone (LHRH) neuronal cells.UniProtKB/Swiss-Prot Function
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Documents
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"NM_001039386" in other vectors (4)
SKU Description Size Price
MC217595 Nsmf (untagged) - Mouse nasal embryonic LHRH factor (Nelf), transcript variant 1, (10ug) 10 ug
$546.00
MG221711 Nsmf (tGFP-tagged) - Mouse nasal embryonic LHRH factor (Nelf) transcript variant 1, (10ug) 10 ug
$695.00
MR221711L3 Lenti ORF clone of Nsmf (Myc-DDK-tagged) - Mouse nasal embryonic LHRH factor (Nelf), transcript variant 1 10 ug
$795.00
MR221711L4 Lenti ORF clone of Nsmf (mGFP-tagged) - Mouse nasal embryonic LHRH factor (Nelf), transcript variant 1 10 ug
$795.00

Plasmid Map for MR221711

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221711
Circular map for MR221711

file-alt Citations

Activation of TrkB signaling mitigates cerebellar anomalies caused by Rbm4-Bdnf deficiency
Tsai, Shen, D et al
Commun Biol (2023) 6 (1), 910 DOI: 10.1038/s42003-023-05294-z
Cited by 5
AI Summary: OriGene products contributed to this study by providing pCMV6-Entry based vectors (empty, PS100001; Nsmf, MR221711; Hsf1, MR208087), which were used to assess BDNF gene expression and investigate the molecular mechanisms underlying cerebellar anomalies in Rbm4-deficient mice.
Snippet: Rbm4 knockdown GC (see above) using primers (Supplementary Table 1). BDNF gene expression assay Three pCMV6-Entry based vectors (empty, PS100001;Nsmf, MR221711;Hsf1, MR208087) were obtained from Origene. NSMF(∆E6) was generated by deleting exon 6 using a PCR-based method;the sequence was verified by Sanger sequencing. Rbm4 was depleted in P7 GCs at DIV1 by transient transfection of pLKO.1
FigureFigureFigure
Showing 1-1 of 1 papers.
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