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Kcnk4 (NM_008431) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221486
Kcnk4 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kcnk4
Synonyms MLZ-622; Tex40; TRAAK; TRAAKt
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221486 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCAGCACCACACTCCTGGCTCTGCTGGCACTGGTGCTGCTTTACTTGGTATCTGGGGCTCTAGTGT
TCCAGGCTCTGGAGCAGCCTCACGAGCAGCAGGCTCAGAAGAAAATGGATCATGGCCGAGACCAGTTTCT
GAGGGACCATCCCTGTGTGAGCCAGAAGAGCCTGGAGGATTTCATCAAGCTCCTGGTTGAAGCCCTGGGA
GGGGGCGCAAACCCAGAAACCAGCTGGACCAATAGCAGCAACCACTCATCAGCTTGGAACCTGGGCAGCG
CCTTCTTTTTCTCGGGGACCATCATCACTACCATCGGCTATGGCAATATAGTCTTACACACAGATGCCGG
GCGTCTCTTTTGTATCTTCTATGCACTGGTGGGGATCCCACTGTTCGGGATGCTGCTGGCGGGAGTCGGG
GACCGGCTGGGCTCCTCTCTGCGCCGGGGCATCGGCCACATCGAAGCAATCTTCTTGAAGTGGCATGTGC
CACCGGGGCTGGTGAGAAGTCTGTCCGCAGTGCTCTTCCTGCTGATCGGCTGCCTGCTCTTTGTCCTCAC
TCCTACCTTCGTGTTCTCCTACATGGAGAGCTGGAGCAAGTTAGAAGCCATCTACTTTGTTATAGTGACT
CTCACCACTGTAGGCTTTGGCGATTATGTACCCGGCGATGGCACCGGGCAGAACTCTCCAGCCTACCAGC
CGCTGGTGTGGTTCTGGATCTTGTTTGGCCTAGCCTACTTCGCCTCAGTGCTCACCACCATCGGCAACTG
GTTGCGAGCAGTGTCCCGCCGAACTCGGGCAGAGATGGGTGGCCTAACGGCACAGGCTGCTAGCTGGACC
GGCACAGTGACAGCGCGAGTGACCCAGCGAACTGGGCCCAGCGCCCCGCCGCCAGAGAAGGAGCAACCAC
TCCTGCCCTCCTCTTTGCCGGCACCGCCTGCTGTTGTTGAGCCAGCCGGCAGGCCCGGCTCCCCTGCACC
CGCAGAGAAGGTTGAGACTCCGTCCCCGCCCACGGCCTCAGCTCTGGATTACCCCAGTGAGAATCTGGCC
TTCATCGACGAGTCCTCAGACACGCAGAGTGAGCGTGGCTGTGCCCTGCCTCGGGCTCCTCGGGGTCGCC
GCCGACCCAACCCATCCAAAAAGCCTTCCAGACCCCGGGGTCCTGGGCGACTCCGAGACAAGGCCGTGCC
GGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221486 protein sequence
Red=Cloning site Green=Tags(s)

MRSTTLLALLALVLLYLVSGALVFQALEQPHEQQAQKKMDHGRDQFLRDHPCVSQKSLEDFIKLLVEALG
GGANPETSWTNSSNHSSAWNLGSAFFFSGTIITTIGYGNIVLHTDAGRLFCIFYALVGIPLFGMLLAGVG
DRLGSSLRRGIGHIEAIFLKWHVPPGLVRSLSAVLFLLIGCLLFVLTPTFVFSYMESWSKLEAIYFVIVT
LTTVGFGDYVPGDGTGQNSPAYQPLVWFWILFGLAYFASVLTTIGNWLRAVSRRTRAEMGGLTAQAASWT
GTVTARVTQRTGPSAPPPEKEQPLLPSSLPAPPAVVEPAGRPGSPAPAEKVETPSPPTASALDYPSENLA
FIDESSDTQSERGCALPRAPRGRRRPNPSKKPSRPRGPGRLRDKAVPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008431
ORF Size 1194 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008431.3
RefSeq Size 1757 bp
RefSeq ORF 1197 bp
Locus ID 16528
UniProt ID O88454
Cytogenetics 19 5.08 cM
MW 43.1 kDa
Summary Voltage-insensitive potassium channel (PubMed:9628867). Channel opening is triggered by mechanical forces that deform the membrane. Channel opening is triggered by raising the intracellular pH to basic levels (By similarity). The channel is inactive at 24 degrees Celsius (in vitro); raising the temperature to 37 degrees Celsius increases the frequency of channel opening, with a further increase in channel activity when the temperature is raised to 42 degrees Celsius (By similarity). Plays a role in the sensory perception of pain caused by pressure (PubMed:19279663). Plays a role in the perception of pain caused by heat (PubMed:19279663).UniProtKB/Swiss-Prot Function
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"NM_008431" in other vectors (4)
SKU Description Size Price
MC208813 Kcnk4 (untagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4), (10ug) 10 ug
$503.00
MG221486 Kcnk4 (tGFP-tagged) - Mouse potassium channel subfamily K member 4 (Kcnk4), (10ug) 10 ug
$657.00
MR221486L3 Lenti ORF clone of Kcnk4 (Myc-DDK-tagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4) 10 ug
$757.00
MR221486L4 Lenti ORF clone of Kcnk4 (mGFP-tagged) - Mouse potassium channel, subfamily K, member 4 (Kcnk4) 10 ug
$757.00

Plasmid Map for MR221486

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221486
Circular map for MR221486

file-alt Citations

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