Dgki (NM_001081206) Mouse Tagged ORF Clone

SKU MR221210

Dgki (Myc-DDK-tagged) - Mouse diacylglycerol kinase, iota (Dgki)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Dgki
• Synonyms: C130010K08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR221210 representing NM_001081206; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATGCTGCGGGAAGGGGCTGCCATTTGCTGCCCCTGCCAGCGGCGCGCGGACCTGCCCGCGCTCCCG; CCGCCTCCAGCGCCCTCAGCCCGACCGGCCTCTGCAGCGGCACCACCTCGGCTTCTTTCGCGGCCGCCGG; AGCGGTCGCCATGAACCCCAGCTCCTCGGCGGGAGAGGAGAGAGGGGCGACGGGTGGCAGCAGCAGCAGC; GGAAGCGGCGCCGGGAGCTGCTGCCTGGGCGCCGAGGGCGGCGCGGACCCGCGGGGTGCAGGGGCAGCGG; CTGCTGCTGCCTTGGAGGAGCCCGCGGCCGCCGGACAGAAAGAGAAGGAAGAGGCGCTGGAGGAGAAGCT; GAGGGACTTAACTTTCCGGAAGCAGGTCTCTTACAGGAAAGCCATCTCCCGGACAGGCCTCCAGCATCTG; GCACCTGCACATCCTCTTGGTCTTCCTGTGGCTAACGGTCCAGCGAAGGAGCCCAGAGCCACTCTGGACT; GGAGCGAGAATGCTGTGAATGGGGAGCACCTGTGGCTGGAGACCAACGTTTCAGGGGACCTCTGCTACCT; GGGGGAGGAGAACTGCCAAGTCCGATTTGCAAAATCCGCTCTCAGGAGGAAGTGTGCCGTCTGCAAAATC; GTGGTCCACACAGCCTGCATTGAGCAACTAGAGAAGATTAATTTCAGATGTAAACCAACATTTCGGGAAG; GAGGCTCCAGATCGCCAAGAGAAAACTTTGTGCGTCACCACTGGGTCCACAGGAGGCGGCAGGAGGGAAA; ATGTAAGCAGTGTGGTAAGGGCTTCCAGCAAAAGTTCTCCTTCCACAGTAAAGAGATCGTGGCCATCAGC; TGTTCTTGGTGCAAGCAGGCGTTTCACAATAAAGTGACCTGCTTCATGCTGCATCATATTGAGGAACCCT; GCTCCCTGGGGGCTCACGCCGCTGTTATTGTCCCGCCCACCTGGATCATTAAGGTGAAGAAACCTCAGAA; CTCCCTGAAGGCTTCCAACCGGAAGAAGAAGAGGACAAGTTTTAAAAGGAAAGCCAGTAAAAGAGGAACT; GAGCAGGAAACCAAAGGCCGTCCTTTTGTGATCAAGCCCATCTCCTCCCCTCTCATGAAGCCTTTGCTTG; TCTTTGTGAACCCAAAGAGTGGGGGAAACCAGGGAACCAAAGTCCTACAGATGTTTATGTGGTACCTGAA; TCCACGGCAAGTCTTTGATCTTTCTCAGGAAGGGCCAAAAGATGCGCTGGAGATGTACAGGAAGGTACCA; AATCTGAGAATTCTGGCCTGCGGTGGGGATGGAACGGTGGGCTGGATCCTCTCCATCCTGGACGAGCTGC; AGCTGAGCCCACAGCCTCCTGTGGGGGTGCTTCCACTGGGGACTGGGAATGACCTGGCTCGGACGCTCAA; CTGGGGAGGGGGTTACACCGATGAGCCTGTTTCTAAGATCCTGTGCCAGGTGGAAGATGGGACCATTGTA; CAGTTGGATCGCTGGAACCTCCACGTAGAGAGAAACCCAGACCTGCCGCCCGAAGAACTTGAAGATGGCG; TGTGTAAGCTTCCTCTGAATGTCTTCAATAATTACTTCAGCCTTGGATTTGATGCTCACGTCACATTGGA; GTTCCATGAATCCCGAGAAGCAAATCCAGAGAAATTCAATAGTCGTTTTCGAAATAAAATGTTCTATGCA; GGGGCAGCCTTTTCTGATTTCCTACAAAGAAGTTCTAGAGATCTATCCAAACACGTCAAAGTTGTCTGTG; ATGGGACAGACCTCACCCCAAAGATCCAGGACCTGAAGTTCCAGTGTATAGTATTTTTAAATATTCCCAG; ATATTGTGCTGGCACAATGCCTTGGGGAAACCCTGGAGATCATCATGACTTTGAACCCCAACGTCATGAT; GACGGCTACATTGAAGTCATTGGATTCACTATGGCCTCTTTGGCTGCCCTGCAAGTTGGGGGGCACGGAG; AAAGGCTGCACCAGTGTCGGGAAGTGATGCTTCTGACTTACAAGTCCATTCCCATGCAAGTGGACGGGGA; GCCATGTCGGTTGGCACCTGCTATGATTCGGATCTCCTTGAGGAATCAGGCCAACATGGTACAGAAGAGC; AAGCGGAGAACCTCGATGCCTTTGCTCAATGACATCCATCAGGTGCAAGCTGCGGACCTGCGGCGAGTGT; CTGCTCCCCCCGGCTCCTTCACCATTCCCCAGTCTGTCCCAGATCGCCTGAGGATCCGTGTGAACAAAAT; CAGTTTACAAGACTATGAAGGACTCCACTATGACAAGGACAAACTCCGGGAAGCTTCCATCCCTCTGGGT; ATTCTAGTTGTTCGTGGAGACTGTGATTTGGAGACTTGCCGTATGTACATAGACCGTCTACAGGAGGACT; TGCAGTCAGTTTCTTCTGGGTCACAGAGAGTTCATTACCAGGACCAAGAAACCTCCTTCCCCAGGGCACT; CTCAGCTCAGAGGCTGTCCCCACGGTGGTGCTTCCTAGATGCAACGTCTGCTGACCGCTTTTATCGAATA; GACAGATCTCAGGAACATTTGCACTTTGTGATGGAGATTTCTCACGATGAAATTTTTATTCTGGACCCAG; ATATGGTGGTGTCACAGCAGGCAGGGACCCCTCCAGGAATGCCTGACCTGGTGGTTGAACAGGCTTCTGG; GCTGTCTGACTGGTGGAACCCTGCCCTGAGGAAACGTATGCTGAGTGACAGTGGGATGATCACACCCCAC; TATGAGGACTCAGATCTGAAAGACTTCAGCCACTCCAGAGTGCTACAGTCCCCAGTCTCTTCAGAAGATC; ATGCAATTTTGCAGGCAGTCCTCACCGGTGACCTTATGAAGCTCATGGAGAGCTATAAGAATGGAGGGAG; CTTACTAATTCAGGGACCAGGGCACTGTTCTCTCCTTCACTACGCAGCTAAGACCGGCAATGGGGACATT; GTGAAGTACATCCTTGACCACGGACCTGCAGAATTACTGGATATGGCAGACAGTGAAACGGGCGAGACTG; CACTGCACAAGGCTGCCTGCCAAAGGAACCGGGCTGTATGCCAGCTTCTGGTGGATGCAGGAGCATCTCT; GAGACAGACAGACTCCAAGGGCAAGACACCCCAGGAACGAGCACAGCAGGCCGGGGACCCAGATTTGGCT; GCTTACCTAGAAAGCCGACAGAACTATAAGATCATTGGCCATGAAGACCTGGAAACTGCTGTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR221210 representing NM_001081206; Red=Cloning site Green=Tags(s); ; MDAAGRGCHLLPLPAARGPARAPAASSALSPTGLCSGTTSASFAAAGAVAMNPSSSAGEERGATGGSSSS; GSGAGSCCLGAEGGADPRGAGAAAAAALEEPAAAGQKEKEEALEEKLRDLTFRKQVSYRKAISRTGLQHL; APAHPLGLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKI; VVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAIS; CSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGT; EQETKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALEMYRKVP; NLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIV; QLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYA; GAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQDLKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHD; DGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKS; KRRTSMPLLNDIHQVQAADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGLHYDKDKLREASIPLG; ILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDQETSFPRALSAQRLSPRWCFLDATSADRFYRI; DRSQEHLHFVMEISHDEIFILDPDMVVSQQAGTPPGMPDLVVEQASGLSDWWNPALRKRMLSDSGMITPH; YEDSDLKDFSHSRVLQSPVSSEDHAILQAVLTGDLMKLMESYKNGGSLLIQGPGHCSLLHYAAKTGNGDI; VKYILDHGPAELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRQTDSKGKTPQERAQQAGDPDLA; AYLESRQNYKIIGHEDLETAV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081206
• ORF Size: 3213 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001081206.2
• RefSeq Size: 4598 bp
• RefSeq ORF: 3216 bp
• Locus ID: 320127
• UniProt ID: D3YWQ0
• Cytogenetics: 6 B1
• MW: 118.7 kDa
• Summary: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Has probably no preference for any of the diacylglycerols in terms of the acyl chain composition, especially for the acyl chain at the sn-2 position (By similarity). By controlling the diacylglycerol/DAG-mediated activation of RASGRP3, negatively regulates the Rap1 signaling pathway (PubMed:15894621). May play a role in presynaptic diacylglycerol/DAG signaling and control neurotransmitter release during metabotropic glutamate receptor-dependent long-term depression (PubMed:21119615).[UniProtKB/Swiss-Prot Function]