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Golga7b (NM_001141983) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221178
Golga7b (Myc-DDK-tagged) - Mouse golgi autoantigen, golgin subfamily a, 7B (Golga7b), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Other products for "Golga7b or NM_001141983"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Golga7b
Synonyms 4933417O08Rik; AI839934
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221178 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACTGAGGTCCACAATCTTCAGGAGCTCAGGCGAAGCGCTTCGCTGGCCACCAAGGTCTTTATCC
AGAGAGACTACAGTGACGGCACCATCTGTCAGTTCCAGACCAAATTCCCGCCAGAGCTGGATAGCCGGAT
TGAGCGGCAGCTCTTTGAGGAGACGGTGAAGACCCTCAATGGTTTTTATGCGGAGGCTGAGAAGATCGGG
GGCAGCTCCTATCTGGAGGGCTGCCTGGCCTGCGCCACGGCCTACTTCATCTTCCTCTGCATGGAGACCC
ACTATGAGAAGGTTCTCAAGAAGATTTCCCGTTACATCCAGGAGCAGAATGAGAAGGTCTTTGCTCCCCG
AGGGCTCCTGCTCACGGATCCGGTGGAACGCGGAATGAGGGTTATCGAGATCTCCATCTATGAGGACCGG
TGCAGCAGTGGTAGTTCCAGTAGCGGCAGCAGCAGCGGCAGCGGCAGCAGCAGCGCTGGCGGGGGAGGGG
CGGGGGCCCGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221178 protein sequence
Red=Cloning site Green=Tags(s)

MATEVHNLQELRRSASLATKVFIQRDYSDGTICQFQTKFPPELDSRIERQLFEETVKTLNGFYAEAEKIG
GSSYLEGCLACATAYFIFLCMETHYEKVLKKISRYIQEQNEKVFAPRGLLLTDPVERGMRVIEISIYEDR
CSSGSSSSGSSSGSGSSSAGGGGAGAR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001141983
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001141983.1, NP_001135455.1
RefSeq Size 2839 bp
RefSeq ORF 504 bp
Locus ID 71146
UniProt ID Q9D428
Cytogenetics 19 C3
MW 18.3 kDa
Summary May be involved in protein transport from Golgi to cell surface.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001141983" in other vectors (4)
SKU Description Size Price
MC211148 Golga7b (untagged) - Mouse golgi autoantigen, golgin subfamily a, 7B (Golga7b), transcript variant 1, (10ug) 10 ug
$330.00
MG221178 Golga7b (tGFP-tagged) - Mouse golgi autoantigen golgin subfamily a 7B (Golga7b) transcript variant 1, (10ug) 10 ug
$500.00
MR221178L3 Lenti ORF clone of Golga7b (Myc-DDK-tagged) - Mouse golgi autoantigen, golgin subfamily a, 7B (Golga7b), transcript variant 1 10 ug
$600.00
MR221178L4 Lenti ORF clone of Golga7b (mGFP-tagged) - Mouse golgi autoantigen, golgin subfamily a, 7B (Golga7b), transcript variant 1 10 ug
$600.00

Plasmid Map for MR221178

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR221178
Circular map for MR221178

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