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Bspry (NM_138653) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221167
Bspry (Myc-DDK-tagged) - Mouse B-box and SPRY domain containing (Bspry)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bspry
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221167 representing NM_138653
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGCGGATGTCTCCGGGACGGAGTCCGGGTCCGAGTCCGGGCCGGAGCCGGAGCCGGGACCGGAGC
CCGGACCGGAGTCCCGACCGGAGTCCCGGCCGAAGCCGGGACCGGGACCGGAGCCCAGACCCGAGTCCGG
GCCGGAGCCGGGGCCGCGATCCGGACTGAGGAGGGGACCGAAGCAGGGGTCCGAGCGGAGCCAGTTGTGC
CCCGAGCACTTCGAGCCTCTGAGCTGGTTCTGCCTCTCGGAGCGACGGCCGGTATGCGCCACCTGCGCCG
GGTTTGGCGGCCGCTGTCACCGGCACCGCATCCGTCGAGCGGAGGAGCACGCGGAGGAGCTGAGAAACAA
GATTGTGGACCAGTGTGAGAGGCTGCAGTTACAGAGTGCTGGCATCTCCAAGTATATGGCCGAGGTCCTG
CAGGGGAAGAACCAGAAAGCAGTGGTCATGGCTAGTGCAGCGAGGGACCTGATCATCCAGAGGCTGAGTC
TGGTGCGGAGCCTGTGTGAGAGTGAGGAACAGCGGTTATTGGAGCAGGTGCACGGCGAAGAGGAGCGGGC
CCACCAGAGTATCCTGACTCAGCGGGCACACTGGGTGGACGCAGTCCAGAAACTGGACACCCTCCGCACC
AACATGGTGGACATGATCACCCACCTGGATGACCTCCAGCTGATTCAGAGGGAACAGGAGATTTTTGAGA
GGGCCGAGGAAGCAGAGGGCATTTTGGATCCCCAGGATTCGGAGAAATTAAGCTTTAATGAGAAGTGTGC
TTGGAGCCCACTACTGACGCAACTGTGGGCAGCATCGGTTCTCGGGTCTCTCTCAGGCGTGGAGGAGGTG
CTGATTGATGAAAGGACTGTCAGCCCCTTACTGCATCTGTCTGAAGATCGAAGGACCCTGACCTTCATTG
CCAAGAAGTCTAAGGTCTGCTCAGATGAACCAGAGCGCTTTGACCACTGGCCCAATGCCCTGGCTGCCAC
TGCCTTCCAGACTGGGCTCCATGCCTGGATAGTGAACGTTAAACACAGTTGTGCTTATAAAGTGGGTGTG
GCCTCAGATCAGCTGCCCCGAAAGGGCTCTGGCAATGACTGCCGCCTGGGCCACAATGCCTTCTCCTGGG
TCTTCTCCCGCTATGATCAAGAGTTCTGTTTCTCACACAATGGGTATCATGAGCCTCTGCCACTACTTCG
GTGCCCAGCACAGCTGGGCATGCTGCTGGACCTGCAGGCAGGAGAGCTGGTCTTCTACGAGCCTGCCTCA
GGGACGGTGCTTCATATCCACCGTGCGTCCTTCCCTCAGGTGCTCTTCCCAGTCTTTGCTGTGGCTGACC
AGCTCATCTCCATCGTTCGCGGACCTCTGGCCACAGTGAGACTAGATTCACCTCCCCATCACCCTTGCAG
CATACGAAGGACAACCTGCCAGGATACTGTTGGCCAGAACGTCGTCGTGGGAGTGTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138653
ORF Size 1458 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138653.1, NP_619594.1
RefSeq Size 1743 bp
RefSeq ORF 1461 bp
Locus ID 192120
UniProt ID Q80YW5
Cytogenetics 4 B3
MW 54.6 kDa
Summary May regulate epithelial calcium transport by inhibiting TRPV5 activity.UniProtKB/Swiss-Prot Function
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"NM_138653" in other vectors (4)
SKU Description Size Price
MC216705 Bspry (untagged) - Mouse B-box and SPRY domain containing (Bspry), (10ug) 10 ug
$503.00
MG221167 Bspry (tGFP-tagged) - Mouse B-box and SPRY domain containing (Bspry), (10ug) 10 ug
$657.00
MR221167L3 Lenti ORF clone of Bspry (Myc-DDK-tagged) - Mouse B-box and SPRY domain containing (Bspry) 10 ug
$757.00
MR221167L4 Lenti ORF clone of Bspry (mGFP-tagged) - Mouse B-box and SPRY domain containing (Bspry) 10 ug
$757.00

Plasmid Map for MR221167

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221167
Circular map for MR221167

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