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Npc1l1 (NM_207242) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221076
Npc1l1 (Myc-DDK-tagged) - Mouse NPC1-like 1 (Npc1l1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,160.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Npc1l1
Synonyms 9130221N23Rik; Gm243
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221076 representing NM_207242
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCTGCCTGGCAGGGATGGCTGCTCTGGGCCCTGCTCCTGAATTCGGCCCAGGGTGAGCTCTACA
CACCCACTCACAAAGCTGGCTTCTGCACCTTTTATGAAGAGTGTGGGAAGAACCCAGAGCTTTCTGGAGG
CCTCACATCACTATCCAATATCTCCTGCTTGTCTAATACCCCAGCCCGCCATGTCACAGGTGACCACCTG
GCTCTTCTCCAGCGCGTCTGTCCCCGCCTATACAATGGCCCCAATGACACCTATGCCTGTTGCTCTACCA
AGCAGCTGGTGTCATTAGACAGTAGCCTGTCTATCACCAAGGCCCTCCTTACACGCTGCCCGGCATGCTC
TGAAAATTTTGTGAGCATACACTGTCATAATACCTGCAGCCCTGACCAGAGCCTCTTCATCAATGTTACT
CGCGTGGTTCAGCGGGACCCTGGACAGCTTCCTGCTGTGGTGGCCTATGAGGCCTTTTATCAACGCAGTT
TTGCAGAGAAGGCCTATGAGTCCTGTAGCCGGGTGCGCATCCCTGCAGCTGCCTCGCTGGCTGTGGGCAG
CATGTGTGGAGTGTATGGCTCTGCCCTCTGCAATGCTCAGCGCTGGCTCAACTTCCAAGGAGACACAGGG
AATGGCCTGGCTCCGCTGGACATCACCTTCCACCTCTTGGAGCCTGGCCAGGCCCTGGCAGATGGGATGA
AGCCACTGGATGGGAAGATCACACCCTGCAATGAGTCCCAGGGTGAAGACTCGGCAGCCTGTTCCTGCCA
GGACTGTGCAGCATCCTGCCCTGTCATCCCTCCGCCCCCGGCCCTGCGCCCTTCTTTCTACATGGGTCGA
ATGCCAGGCTGGCTGGCTCTCATCATCATCTTCACTGCTGTCTTTGTATTGCTCTCTGTTGTCCTTGTGT
ATCTCCGAGTGGCTTCCAACAGGAACAAGAACAAGACAGCAGGCTCCCAGGAAGCCCCCAACCTCCCTCG
TAAGCGCAGATTCTCACCTCACACTGTCCTTGGCCGGTTCTTCGAGAGCTGGGGAACAAGGGTGGCCTCA
TGGCCACTCACTGTCTTGGCACTGTCCTTCATAGTTGTGATAGCCTTGTCAGTAGGCCTGACCTTTATAG
AACTCACCACAGACCCTGTGGAACTGTGGTCGGCCCCTAAAAGCCAAGCCCGGAAAGAAAAGGCTTTCCA
TGACGAGCATTTTGGCCCCTTCTTCCGAACCAACCAGATTTTTGTGACAGCTAAGAACAGGTCCAGCTAC
AAGTACGACTCCCTGCTGCTAGGGCCCAAGAACTTCAGTGGGATCCTATCCCTGGACTTGCTGCAGGAGC
TGTTGGAGCTACAGGAGAGACTTCGACACCTGCAAGTGTGGTCCCATGAGGCACAGCGCAACATCTCCCT
CCAGGACATCTGCTATGCTCCCCTCAAACCGCATAACACCAGCCTCACTGACTGCTGTGTCAACAGCCTC
CTTCAATACTTCCAGAACAACCACACACTCCTGCTGCTCACAGCCAACCAGACTCTGAATGGCCAGACCT
CCCTGGTGGACTGGAAGGACCATTTCCTCTACTGTGCCAATGCCCCTCTCACGTACAAAGATGGCACAGC
CCTGGCCCTGAGCTGCATAGCTGACTACGGGGCACCTGTCTTCCCCTTCCTTGCTGTTGGGGGCTACCAA
GGGACGGACTACTCGGAGGCAGAAGCCCTGATCATAACCTTCTCTATCAATAACTACCCCGCTGATGATC
CCCGCATGGCCCACGCCAAGCTCTGGGAGGAGGCTTTCTTGAAGGAAATGCAATCCTTCCAGAGAAGCAC
AGCTGACAAGTTCCAGATTGCGTTCTCAGCTGAGCGTTCTCTGGAGGACGAGATCAATCGCACTACCATC
CAGGACCTGCCTGTCTTTGCCATCAGCTACCTTATCGTCTTCCTGTACATCTCCCTGGCCCTGGGCAGCT
ACTCCAGATGGAGCCGAGTTGCGGTGGATTCCAAGGCTACTCTGGGCCTAGGTGGGGTGGCTGTTGTGCT
GGGAGCAGTCGTGGCTGCCATGGGCTTCTACTCCTACCTGGGTGTCCCCTCCTCTCTGGTCATCATTCAA
GTGGTACCTTTCCTGGTGCTGGCTGTGGGAGCTGACAACATCTTCATCTTTGTTCTTGAGTACCAGAGGC
TGCCTAGGATGCCCGGGGAGCAGCGAGAGGCTCACATTGGCCGCACCCTGGGTAGTGTGGCCCCCAGCAT
GCTGCTGTGCAGCCTCTCTGAGGCCATCTGCTTCTTTCTAGGGGCCCTGACCTCCATGCCAGCTGTGAGG
ACCTTTGCCTTGACCTCTGGCTTAGCAATCATCTTTGACTTCCTGCTCCAGATGACAGCCTTTGTGGCCC
TGCTCTCCCTGGATAGCAAGAGGCAGGAGGCCTCTCGCCCCGACGTCGTGTGCTGCTTTTCAAGCCGAAA
TCTGCCCCCACCGAAACAAAAAGAAGGCCTCTTACTTTGCTTCTTCCGCAAGATATACACTCCCTTCCTG
CTGCACAGATTCATCCGCCCTGTTGTGCTGCTGCTCTTTCTGGTCCTGTTTGGAGCAAACCTCTACTTAA
TGTGCAACATCAGCGTGGGGCTGGACCAGGATCTGGCTCTGCCCAAGGATTCCTACCTGATAGACTACTT
CCTCTTTCTGAACCGGTACTTGGAAGTGGGGCCTCCAGTGTACTTTGACACCACCTCAGGCTACAACTTT
TCCACCGAGGCAGGCATGAACGCCATTTGCTCTAGTGCAGGCTGTGAGAGCTTCTCCCTAACCCAGAAAA
TCCAGTATGCCAGTGAATTCCCTAATCAGTCTTATGTGGCTATTGCTGCATCCTCCTGGGTAGATGACTT
CATCGACTGGCTGACCCCATCCTCCTCCTGCTGCCGCATTTATACCCGTGGCCCCCATAAAGATGAGTTC
TGTCCCTCAACGGATACTTCCTTCAACTGTCTCAAAAACTGCATGAACCGCACTCTGGGTCCCGTGAGAC
CCACAACAGAACAGTTTCATAAGTACCTGCCCTGGTTCCTGAATGATACGCCCAACATCAGATGTCCTAA
AGGGGGCCTAGCAGCGTATAGAACCTCTGTGAATTTGAGCTCAGATGGCCAGATTATAGCCTCCCAGTTC
ATGGCCTACCACAAGCCCTTACGGAACTCACAGGACTTTACAGAAGCTCTCCGGGCATCCCGGTTGCTAG
CAGCCAACATCACAGCTGAACTACGGAAGGTGCCTGGGACAGATCCCAACTTTGAGGTCTTCCCTTACAC
GATCTCCAATGTGTTCTACCAGCAATACCTGACGGTTCTCCCTGAGGGAATCTTCACTCTTGCTCTCTGC
TTCGTGCCCACCTTTGTGGTCTGCTACCTCCTACTGGGCCTGGACATACGCTCAGGCATCCTCAACCTGC
TCTCCATCATTATGATCCTCGTGGACACCATCGGCCTCATGGCTGTGTGGGGTATCAGCTACAATGCTGT
GTCCCTCATCAACCTTGTCACGGCAGTGGGCATGTCTGTGGAGTTCGTGTCCCACATTACCCGGTCCTTT
GCTGTAAGCACCAAGCCTACCCGGCTGGAGAGAGCCAAAGATGCTACTATCTTCATGGGCAGTGCGGTGT
TTGCTGGAGTGGCCATGACCAACTTCCCGGGCATCCTCATCCTGGGCTTTGCTCAGGCCCAGCTTATCCA
GATTTTCTTCTTCCGCCTCAACCTCCTGATCACCTTGCTGGGTCTGCTACACGGCCTGGTCTTCCTGCCC
GTTGTCCTCAGCTATCTGGGGCCAGATGTTAACCAAGCTCTGGTACTGGAGGAGAAACTAGCCACTGAGG
CAGCCATGGTCTCAGAGCCTTCTTGCCCACAGTACCCCTTCCCGGCTGATGCAAACACCAGTGACTATGT
TAACTACGGCTTTAATCCAGAATTTATCCCTGAAATTAATGCTGCTAGCAGCTCTCTGCCCAAAAGTGAC
CAAAAGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221076 representing NM_207242
Red=Cloning site Green=Tags(s)

MAAAWQGWLLWALLLNSAQGELYTPTHKAGFCTFYEECGKNPELSGGLTSLSNISCLSNTPARHVTGDHL
ALLQRVCPRLYNGPNDTYACCSTKQLVSLDSSLSITKALLTRCPACSENFVSIHCHNTCSPDQSLFINVT
RVVQRDPGQLPAVVAYEAFYQRSFAEKAYESCSRVRIPAAASLAVGSMCGVYGSALCNAQRWLNFQGDTG
NGLAPLDITFHLLEPGQALADGMKPLDGKITPCNESQGEDSAACSCQDCAASCPVIPPPPALRPSFYMGR
MPGWLALIIIFTAVFVLLSVVLVYLRVASNRNKNKTAGSQEAPNLPRKRRFSPHTVLGRFFESWGTRVAS
WPLTVLALSFIVVIALSVGLTFIELTTDPVELWSAPKSQARKEKAFHDEHFGPFFRTNQIFVTAKNRSSY
KYDSLLLGPKNFSGILSLDLLQELLELQERLRHLQVWSHEAQRNISLQDICYAPLKPHNTSLTDCCVNSL
LQYFQNNHTLLLLTANQTLNGQTSLVDWKDHFLYCANAPLTYKDGTALALSCIADYGAPVFPFLAVGGYQ
GTDYSEAEALIITFSINNYPADDPRMAHAKLWEEAFLKEMQSFQRSTADKFQIAFSAERSLEDEINRTTI
QDLPVFAISYLIVFLYISLALGSYSRWSRVAVDSKATLGLGGVAVVLGAVVAAMGFYSYLGVPSSLVIIQ
VVPFLVLAVGADNIFIFVLEYQRLPRMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTSMPAVR
TFALTSGLAIIFDFLLQMTAFVALLSLDSKRQEASRPDVVCCFSSRNLPPPKQKEGLLLCFFRKIYTPFL
LHRFIRPVVLLLFLVLFGANLYLMCNISVGLDQDLALPKDSYLIDYFLFLNRYLEVGPPVYFDTTSGYNF
STEAGMNAICSSAGCESFSLTQKIQYASEFPNQSYVAIAASSWVDDFIDWLTPSSSCCRIYTRGPHKDEF
CPSTDTSFNCLKNCMNRTLGPVRPTTEQFHKYLPWFLNDTPNIRCPKGGLAAYRTSVNLSSDGQIIASQF
MAYHKPLRNSQDFTEALRASRLLAANITAELRKVPGTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALC
FVPTFVVCYLLLGLDIRSGILNLLSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSF
AVSTKPTRLERAKDATIFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLP
VVLSYLGPDVNQALVLEEKLATEAAMVSEPSCPQYPFPADANTSDYVNYGFNPEFIPEINAASSSLPKSD
QKF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207242
ORF Size 3999 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207242.2, NP_997125.2
RefSeq Size 4571 bp
RefSeq ORF 4002 bp
Locus ID 237636
Cytogenetics 11 A1
MW 147.6 kDa
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"NM_207242" in other vectors (4)
SKU Description Size Price
MC224273 Npc1l1 (untagged) - Mouse NPC1-like 1 (Npc1l1), (10ug) 10 ug
$1,255.00
MG221076 Npc1l1 (tGFP-tagged) - Mouse NPC1-like 1 (Npc1l1), (10ug) 10 ug
$1,360.00
MR221076L3 Lenti ORF clone of Npc1l1 (Myc-DDK-tagged) - Mouse NPC1-like 1 (Npc1l1) 10 ug
$1,460.00
MR221076L4 Lenti ORF clone of Npc1l1 (mGFP-tagged) - Mouse NPC1-like 1 (Npc1l1) 10 ug
$1,460.00

Plasmid Map for MR221076

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221076
Circular map for MR221076

file-alt Citations

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