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Cep295nl (NM_021440) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221032
BC100451 (Myc-DDK-tagged) - Mouse cDNA sequence BC100451 (BC100451)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $496.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Cep295nl or NM_021440"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cep295nl
Synonyms Ddc8; FS39; Kiaa1731nl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221032 representing NM_021440
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGAGACACGGAGCGAGCAGCTCAGCTGAGCCCTTCTTCCGAAGATGAAGCCTTAGTTTTGAGGC
AGAAGCCGTTAGAGATGCCAGCCCAAGAGGAGGACAGTACCACTCTGCAGCAGTGGAAAGCACGGCAGCT
GCAACGCCTGGCTGAGGAGTTAAAGGCAGAGTGGCAGGAGGCCCGGCTGCAGCAGGTCCGACAGGCTGAA
CGCCTCTACTTATCCCACCTGTTGGACGAGGCAGCCGAGCGGAGCATGGGAAACGATCCCAGCGTGCATG
AACAGAACCAGAGAAGAACAGCAAAACACACAAGGGCTAAAGAGAGAAACCGAGCAGCTTTCAGAGAGGA
GCGGGGCCGCAGGGAGGAGCACCCCAGGCAGCACCCCAAGTCCCGGAAAAAGGCACCATGCTCAGAGAGA
CGCAGTTCTGCCAAAGCCCGGGGCCCAGCCTCCGGTGAGAAGGGTAAAAGGAGACGGGTGTCTTCAAGTA
AGGACCACGATGGCTACCAGGGCCCTCGGGTGACCAGAAGGGTAGGTGTGGCAAAGCTCAACCCATTCTT
TGATGGAGACACTGACTGCATGGAGGACGTGCAGAAGGAATTCTTCCGGGAGGGGAGGCGGCCCTCGGCA
AAGGGGACTCACAACCTCAGGGACCAGAGTCTACAGGGCAAAACAACAGCCCTAACTCAGCCCTTGCTCC
ACGGCCCCACCTGCAAACAAGAGGCTGCAGCCCAGGAGCCCCCAAGCAAGTACAACAAGAATCTGTGGCA
CAAAGAAATCGAATCTACCTTTGAAGAATTGTTTAACATGAATAGGAAGCTGAAGAAACATTTGAATTTG
CACCTGGAACAGAGGCTCAAGGCAGACCAGAATCCAGATGAGCAGCAGAGCTACTCAGAGATTCGGAGTG
AGACCTTTGGCACCCCGAGAGAGGAGAGGACGGAGGAGGTAGAAACCGCCGAGGAGTCTGGGAGCCCAAC
CGAGGTGGAGACCACTGAGATGTGGTCCAAAGTCAATCTGAAACAGATACTGAGTGACTCAGAGTACCCC
AGATACCAGCAGATAGCAAAGTACCCGTTAAAGAGTGAAAGCCTAGTGCCTGTCAAGGCAGGGACATCTA
GAGAACAAGATGACTTACTCTCACTAAGCCCAGAGTCAGGACAGGAGCCGCCCAAGTCACCTTTGTTGGA
GGACGAAAGCCTCAAGCCTTACCTGCAGAAACAGGCAGACTCTGTAGCAAGCTGGATGGCTTTGAGGCAA
AAGCAGAAGGCAGAGTTGGAGCAGAGAAGGCAAAAGGCACTTTTGGAGCTGACAGAACACCCCAACATGA
GCTTGGAGATCCACTACAAGGCTGAGCTGGAGGAAGAACGCCGGGCGCGTAGACGGATGCGCTTGGCTCT
CCTCAAATCTAATTCCACTGGGATCTGTGCCCTGCCCCCTGACCGAAACAACCTGTCCCTGGACAACGGC
CTTCTCGATGAGGACAAACAGAACCAGATGATTCGTGACCTCCAGCAGCAGATCTTAGAGCAAAACAAGT
TACACCAGGAGTTTTTGGAAAAAGCCAGGAAACGCTTGCAAGAGTTTCAAAAATCATTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221032 representing NM_021440
Red=Cloning site Green=Tags(s)

MQRDTERAAQLSPSSEDEALVLRQKPLEMPAQEEDSTTLQQWKARQLQRLAEELKAEWQEARLQQVRQAE
RLYLSHLLDEAAERSMGNDPSVHEQNQRRTAKHTRAKERNRAAFREERGRREEHPRQHPKSRKKAPCSER
RSSAKARGPASGEKGKRRRVSSSKDHDGYQGPRVTRRVGVAKLNPFFDGDTDCMEDVQKEFFREGRRPSA
KGTHNLRDQSLQGKTTALTQPLLHGPTCKQEAAAQEPPSKYNKNLWHKEIESTFEELFNMNRKLKKHLNL
HLEQRLKADQNPDEQQSYSEIRSETFGTPREERTEEVETAEESGSPTEVETTEMWSKVNLKQILSDSEYP
RYQQIAKYPLKSESLVPVKAGTSREQDDLLSLSPESGQEPPKSPLLEDESLKPYLQKQADSVASWMALRQ
KQKAELEQRRQKALLELTEHPNMSLEIHYKAELEEERRARRRMRLALLKSNSTGICALPPDRNNLSLDNG
LLDEDKQNQMIRDLQQQILEQNKLHQEFLEKARKRLQEFQKSF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021440
ORF Size 1599 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021440.2, NP_067415.2
RefSeq Size 1993 bp
RefSeq ORF 1602 bp
Locus ID 58251
UniProt ID Q497N6
Cytogenetics 11 E2
MW 62 kDa
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Documents
Resources
"NM_021440" in other vectors (4)
SKU Description Size Price
MC217598 BC100451 (untagged) - Mouse cDNA sequence BC100451 (BC100451), (10ug) 10 ug
$547.00
MG221032 BC100451 (tGFP-tagged) - Mouse differential display clone 8 (Ddc8), (10ug) 10 ug
$696.00
MR221032L3 Lenti ORF clone of BC100451 (Myc-DDK-tagged) - Mouse cDNA sequence BC100451 (BC100451) 10 ug
$796.00
MR221032L4 Lenti ORF clone of BC100451 (mGFP-tagged) - Mouse cDNA sequence BC100451 (BC100451) 10 ug
$796.00

Plasmid Map for MR221032

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221032
Circular map for MR221032

file-alt Citations

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