Apobec2 (NM_009694) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR221030
Apobec2 (Myc-DDK-tagged) - Mouse apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2 (Apobec2)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Apobec2
Synonyms Arp1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221030 representing NM_009694
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGAAGGAAGAGGCCGCTGAGGCTGCGGCGCCAGCCTCTCAGAATGGAGATGATTTGGAGAACC
TGGAAGACCCTGAGAAGCTGAAAGAGCTGATCGATCTTCCGCCCTTCGAGATTGTCACTGGGGTGCGGCT
ACCAGTCAACTTCTTCAAGTTTCAGTTCCGGAACGTGGAATACAGTTCCGGGCGGAATAAGACCTTTCTC
TGCTATGTGGTCGAAGTACAGAGTAAGGGCGGCCAAGCGCAGGCGACGCAGGGCTACCTGGAGGATGAAC
ACGCAGGTGCCCACGCCGAGGAGGCTTTCTTTAACACCATCCTGCCAGCTTTCGACCCGGCCCTCAAGTA
CAATGTCACCTGGTATGTGTCCTCCAGCCCCTGTGCAGCCTGCGCTGACCGGATTCTCAAAACCCTCAGC
AAGACTAAGAACCTTCGTCTGCTCATTCTGGTGAGCCGGCTCTTCATGTGGGAGGAGCCAGAGGTCCAGG
CCGCTCTGAAAAAGCTGAAGGAGGCCGGCTGCAAACTTCGCATCATGAAACCCCAGGACTTCGAGTACAT
CTGGCAGAATTTTGTGGAGCAAGAAGAGGGTGAATCCAAGGCCTTTGAGCCCTGGGAGGACATTCAGGAG
AACTTCCTATACTATGAGGAGAAGTTGGCAGACATCCTGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR221030 representing NM_009694
Red=Cloning site Green=Tags(s)

MAQKEEAAEAAAPASQNGDDLENLEDPEKLKELIDLPPFEIVTGVRLPVNFFKFQFRNVEYSSGRNKTFL
CYVVEVQSKGGQAQATQGYLEDEHAGAHAEEAFFNTILPAFDPALKYNVTWYVSSSPCAACADRILKTLS
KTKNLRLLILVSRLFMWEEPEVQAALKKLKEAGCKLRIMKPQDFEYIWQNFVEQEEGESKAFEPWEDIQE
NFLYYEEKLADILK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009694
ORF Size 672 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009694.3, NP_033824.1
RefSeq Size 1218 bp
RefSeq ORF 675 bp
Locus ID 11811
UniProt ID Q9WV35
Cytogenetics 17 24.02 cM
MW 26.1 kDa
Summary Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009694" in other vectors (6)
SKU Description Size Price
MC208137 Apobec2 (untagged) - Mouse apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2 (Apobec2), (10ug) 10 ug
$480.00
MG221030 Apobec2 (tGFP-tagged) - Mouse apolipoprotein B mRNA editing enzyme catalytic polypeptide 2 (Apobec2), (10ug) 10 ug
$650.00
MR221030L1 Lenti ORF clone of Apobec2 (Myc-DDK-tagged) - Mouse apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2 (Apobec2) 10 ug
$750.00
MR221030L2 Lenti ORF clone of Apobec2 (mGFP-tagged) - Mouse apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2 (Apobec2) 10 ug
$750.00
MR221030L3 Lenti ORF clone of Apobec2 (Myc-DDK-tagged) - Mouse apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2 (Apobec2) 10 ug
$750.00
MR221030L4 Lenti ORF clone of Apobec2 (mGFP-tagged) - Mouse apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2 (Apobec2) 10 ug
$750.00

Plasmid Map for MR221030

Download sequence for

Cloning scheme for MR221030

Circular map for MR221030

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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