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Kcnd3 (NM_019931) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR221003
Kcnd3 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, Shal-related family, member 3 (Kcnd3), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $610.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kcnd3
Synonyms AW045978; Kncd3; Kv4.3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR221003 representing NM_019931
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGGAGTTGCAGCCTGGCTGCCTTTTGCCCGGGCTGCGGCCATTGGATGGATGCCAGTGGCCA
ACTGCCCCATGCCCCTAGCTCCAGCGGACAAGAACAAGCGGCAAGACGAGCTGATCGTCCTCAACGTAAG
TGGACGACGGTTCCAGACCTGGAGGACCACTCTGGAGCGCTATCCCGACACCTTGCTGGGTAGCACAGAG
AAGGAGTTCTTCTTCAATGAGGACACGAAGGAGTACTTCTTTGACCGTGACCCGGAAGTGTTCCGTTGTG
TGCTTAACTTCTACCGCACCGGGAAGCTGCACTACCCACGCTATGAATGCATCTCTGCCTACGACGATGA
ACTGGCCTTCTATGGCATCCTCCCCGAGATCATCGGAGACTGCTGCTACGAGGAGTACAAAGACCGCAAG
CGGGAGAATGCGGAGCGGCTCATGGATGACAATGACTCTGAAAACAACCAGGAGTCCATGCCCTCTCTCA
GCTTCCGTCAGACCATGTGGCGGGCCTTCGAGAACCCACACACCAGCACCCTGGCACTGGTCTTCTACTA
TGTGACTGGCTTCTTCATTGCCGTCTCGGTCATCACCAATGTGGTGGAGACGGTGCCTTGCGGCACCGTG
CCTGGGAGCAAGGAGCTGCCGTGTGGAGAGCGCTATTCGGTGGCTTTCTTTTGCCTGGACACTGCCTGTG
TAATGATCTTCACAGTGGAGTACCTCCTCCGACTCTTTGCGGCACCCAGCAGATACCGCTTCATCCGCAG
TGTGATGAGCATTATCGACGTTGTGGCCATCATGCCCTATTACATTGGCCTGGTCATGACCAACAATGAG
GACGTGTCTGGGGCATTTGTCACACTCCGGGTCTTCCGTGTCTTCAGGATCTTCAAGTTCTCCCGACATT
CCCAGGGTCTACGGATCCTGGGCTACACCCTGAAGAGCTGTGCCTCAGAACTGGGCTTTCTTCTCTTCTC
CCTTACCATGGCCATCATCATCTTTGCCACTGTGATGTTTTATGCTGAGAAGGGCTCCTCTGCCAGCAAG
TTCACAAGCATCCCTGCATCTTTCTGGTACACCATAGTCACCATGACTACACTGGGATATGGAGACATGG
TGCCTAAGACAATCGCAGGGAAGATATTTGGCTCCATCTGCTCCCTCAGCGGTGTCCTGGTCATTGCCCT
GCCGGTCCCTGTGATAGTCTCCAACTTTAGCAGGATCTACCACCAGAATCAGAGAGCAGATAAACGCAGG
GCACAAAAGAAGGCCCGCCTTGCCAGGATCCGGGTGGCCAAAACGGGAAGCTCCAATGCCTACCTGCACA
GCAAGCGCAATGGGCTCCTCAATGAGGCCCTGGAGCTGACGGGCACCCCAGAAGAGGAGCAGATGGGCAA
GACCACCTCACTCATCGAGAGCCAGCACCACCACCTGCTACACTGCTTAGAAAAGACCACTGGGTTATCC
TATCTTGTGGATGATCCCCTGTTATCTGTACGAACCTCCACCATCAAGAACCATGAGTTTATTGATGAGC
AGATGTTTGAGCAGAACTGCATGGAGAGCTCGATGCAGAACTACCCCTCCACCAGAAGTCCTTCTCTGTC
CAGCCACTCGGGCCTCACCACCACCTGCTGCTCCCGTCGTAGCAAGAAGACCACACACCTGCCCAACTCT
AACCTGCCTGCCACCCGCCTGCGCAGCATGCAGGAGCTCAGCACGCTCCACATCCAGGGCAGCGAGCAGC
CCTCCCTCACCACCAGTCGCTCCAGCCTTAATTTGAAAGCAGACGATGGACTGAGACCAAACTGCAAAAC
ATCCCAGATTACCACGGCCATCATCAGCATCCCCACCCCCCCAGCTCTGACCCCCGAGGGAGAAAGTCGG
CCACCCCCTGCCAGCCCAGGCCCCAACACGAACATTCCTTCCATAACCAGCAATGTCGTCAAGGTCTCTG
CCTTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR221003 representing NM_019931
Red=Cloning site Green=Tags(s)

MAAGVAAWLPFARAAAIGWMPVANCPMPLAPADKNKRQDELIVLNVSGRRFQTWRTTLERYPDTLLGSTE
KEFFFNEDTKEYFFDRDPEVFRCVLNFYRTGKLHYPRYECISAYDDELAFYGILPEIIGDCCYEEYKDRK
RENAERLMDDNDSENNQESMPSLSFRQTMWRAFENPHTSTLALVFYYVTGFFIAVSVITNVVETVPCGTV
PGSKELPCGERYSVAFFCLDTACVMIFTVEYLLRLFAAPSRYRFIRSVMSIIDVVAIMPYYIGLVMTNNE
DVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGSSASK
FTSIPASFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRR
AQKKARLARIRVAKTGSSNAYLHSKRNGLLNEALELTGTPEEEQMGKTTSLIESQHHHLLHCLEKTTGLS
YLVDDPLLSVRTSTIKNHEFIDEQMFEQNCMESSMQNYPSTRSPSLSSHSGLTTTCCSRRSKKTTHLPNS
NLPATRLRSMQELSTLHIQGSEQPSLTTSRSSLNLKADDGLRPNCKTSQITTAIISIPTPPALTPEGESR
PPPASPGPNTNIPSITSNVVKVSAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019931
ORF Size 1965 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019931.2
RefSeq Size 1968 bp
RefSeq ORF 1968 bp
Locus ID 56543
UniProt ID Q9Z0V1
Cytogenetics 3 F2.2
MW 73.9 kDa
Summary Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019931" in other vectors (4)
SKU Description Size Price
MC220177 Kcnd3 (untagged) - Mouse potassium voltage-gated channel, Shal-related family, member 3 (Kcnd3), transcript variant 2, (10ug) 10 ug
$672.00
MG221003 Kcnd3 (tGFP-tagged) - Mouse potassium voltage-gated channel Shal-related family member 3 (Kcnd3) transcript variant 2, (10ug) 10 ug
$489.00 $810.00
MR221003L3 Lenti ORF clone of Kcnd3 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, Shal-related family, member 3 (Kcnd3), transcript variant 2 10 ug
$910.00
MR221003L4 Lenti ORF clone of Kcnd3 (mGFP-tagged) - Mouse potassium voltage-gated channel, Shal-related family, member 3 (Kcnd3), transcript variant 2 10 ug
$910.00

Plasmid Map for MR221003

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR221003
Circular map for MR221003

file-alt Citations

Kv4. 1, a key ion channel for low frequency firing of dentate granule cells, is crucial for pattern separation
Kim, Lee, Kim et al
bioRxiv (2019)
Snippet: 6 (DNA/lipid). The GFP-tagged Kv4.1 (Cat# MG220056), GFP-tagged Kv4.2 (Cat# MG209597) and Myc-tagged Kv4.3 (Cat# MR221003) expression constructs were purchased from OriGene (Rockville, MD, USA). Transfected View as HTML
Showing 1-1 of 1 papers.
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