Npffr2 (NM_133192) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR220938
Npffr2 (Myc-DDK-tagged) - Mouse neuropeptide FF receptor 2 (Npffr2)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Npffr2
Synonyms Gpr74; HG31; NPFF2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220938 representing NM_133192
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGAGAAATGGGACTCAAACTCTTCAGAAAGCTGGAATCACATCTGGAGTGGCAATGATACACAGC
ATCACTGGTATTCAGATATCAACATTACCTATGTGAACTACTATCTCCACCAGCCCCAAGTGGCAGCTGT
CTTCATCAGCTCCTACCTCCTGATCTTTGTCTTGTGCATGGTGGGAAATACTGTCGTTTGCTTTATTGTG
ATAAGGAATAGACACATGCACACAGTCACTAATTTCTTCATCTTAAACCTTGCCATAAGTGATTTACTGG
TTGGAATATTCTGTATGCCTATCACATTGCTGGACAACATCATAGCAGGATGGCCATTCGGAAGCAGCAT
GTGCAAGATCAGTGGGCTGGTGCAAGGGATATCAGTTGCGGCTTCCGTCTTCACCTTGGTTGCAATAGCT
GTGGACAGATTCCGCTGTGTGGTCTACCCCTTTAAGCCAAAGCTCACTGTCAAGACAGCCTTTGTCACGA
TTGTGATCATCTGGGGCCTGGCCATCGCCATTATGACTCCATCTGCAATAATGTTACATGTACAAGAAGA
AAAATACTACCGTGTGAGACTCAGCTCCCACAATAAAACCAGCACAGTCTACTGGTGTCGGGAGGACTGG
CCAAGACACGAAATGAGGAGGATCTATACCACGGTGCTATTTGCCACCATCTATCTTGCTCCTCTCTCAC
TCATTGTTATCATGTATGCAAGGATTGGGGCTTCCCTCTTCAAGACGGCAGCACACTGCACAGGCAAGCA
GCGTCCAGTGCAGTGGCATGTATCCAAGAAGAAACAGAAGGTCATCAAGATGCTGCTGACTGTGGCCCTC
CTTTTCATCCTTTCCTGGCTTCCCCTGTGGACCCTGATGATGCTCTCAGACTATACTGACCTGTCTCCTA
ACAAACTGCGTATCATCAACATCTACATCTACCCTTTCGCCCACTGGCTCGCCTTCTGCAACAGCAGTGT
CAACCCTATTATTTATGGATTCTTTAATGAAAATTTTCGCAATGGTTTCCAAGATGCTTTCCAGATCTGC
CAAAAGAAAGCCAAGCCCCAGGAAGCCTATTCCCTGAGAGCGAAACGCAACATAGTCATAAACACATCGG
GCCTGCTGGTGCAGGAACCGGTGTCTCAAAACCCAGGTGGGGAAAATTTGGGATGTGGAAAAAGTGCAGA
CAATCCCACACAGGAATCCTTGATAGAGGAAATGGGAGAAGCTACTAACAGTACTGTGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR220938 representing NM_133192
Red=Cloning site Green=Tags(s)

MSEKWDSNSSESWNHIWSGNDTQHHWYSDINITYVNYYLHQPQVAAVFISSYLLIFVLCMVGNTVVCFIV
IRNRHMHTVTNFFILNLAISDLLVGIFCMPITLLDNIIAGWPFGSSMCKISGLVQGISVAASVFTLVAIA
VDRFRCVVYPFKPKLTVKTAFVTIVIIWGLAIAIMTPSAIMLHVQEEKYYRVRLSSHNKTSTVYWCREDW
PRHEMRRIYTTVLFATIYLAPLSLIVIMYARIGASLFKTAAHCTGKQRPVQWHVSKKKQKVIKMLLTVAL
LFILSWLPLWTLMMLSDYTDLSPNKLRIINIYIYPFAHWLAFCNSSVNPIIYGFFNENFRNGFQDAFQIC
QKKAKPQEAYSLRAKRNIVINTSGLLVQEPVSQNPGGENLGCGKSADNPTQESLIEEMGEATNSTVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133192
ORF Size 1251 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133192.3, NP_573455.2
RefSeq Size 1643 bp
RefSeq ORF 1254 bp
Locus ID 104443
UniProt ID Q924H0
Cytogenetics 5 E1
MW 47.9 kDa
Summary Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133192" in other vectors (4)
SKU Description Size Price
MC212100 Npffr2 (untagged) - Mouse neuropeptide FF receptor 2 (Npffr2), (10ug) 10 ug
$686.00
MG220938 Npffr2 (tGFP-tagged) - Mouse neuropeptide FF receptor 2 (Npffr2), (10ug) 10 ug
$886.00
MR220938L3 Lenti ORF clone of Npffr2 (Myc-DDK-tagged) - Mouse neuropeptide FF receptor 2 (Npffr2) 10 ug
$986.00
MR220938L4 Lenti ORF clone of Npffr2 (mGFP-tagged) - Mouse neuropeptide FF receptor 2 (Npffr2) 10 ug
$986.00

Plasmid Map for MR220938

Download sequence for

Cloning scheme for MR220938

Circular map for MR220938

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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