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5330417C22Rik (NM_001033304) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220873
5330417C22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5330417C22 gene (5330417C22Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $834.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "5330417C22Rik or NM_001033304"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 5330417C22Rik
Synonyms BB183350
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220873 representing NM_001033304
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGACCAGTCCTGCAAGCCCTGCGCGGAGGGCCGCTACTCCCTCGGCACTGGCATCCGATTTGATG
AGTGGGATGAGCTACCTCACGGCTTTGCCAGCCTCTCGGCCAACTTGGAGGTCGATGACAGCATCTCGGA
GTCCACTGAGAACTGCACTTCGTCCAAGTGGGTTCCCCGGGGAGACTACATCGCCTCCAACACAGATGAA
TGCACCGCCACACTGATGTATGCCGTCAACCTAAAGCAGTCTGGCACCGTTAACTTTGAGTACTACTACC
CGGACTCCAGCATCATCTTTGAATTTTTTGTCCAGAATGACCAGTGCCAGCCCAGTGCTGATGACTCCAG
GTGGATGAAGACTACGGAGAAAGGATGGGAATTCCACAGTGTTGAGCTAAATAGAGGCAATAACGTCCTC
TATTGGAGAACCACAGCTTTCTCGGTGTGGTCCAAAGTGTCCAAGCCCGTGCTTGTTAGAAACATCGCCA
TAACAGGGGTGGCTTACACCTCCGAGTGTTTCCCCTGCAAGCCTGGCACATACGCTGCCAAGCAGGGCTC
CCCTTTCTGCAAGCTTTGTCCAGCCAACTACTACTCAAACAAAGGAGAAACGTCCTGCCATCCGTGTGAT
GCTGACAAATACTCAGAAAAAGGATCTTCCACCTGCAAAGTGCGCCCAGCCTGCACAGACAAGGACTATT
TCTACACCCACACAGCCTGTGATGCTCAGGGAGAGACACAGCTCATGTACAAATGGGCCATACCAAAAAT
CTGCGGTGAGGACCTGGAGGGGGCGGTGAAGCTGCCCGCCTCTGGTGTGAAGACCCGCTGCCCACCCTGT
AACCCGGGCTTCTTCAAAACCGACAACAGCACCTGTGAACCTTGCCCATACGGCTCGTACTCCAACGGCT
CGGATTGTACCCACTGCCCAGCAGGAACTGAGCCTGCCGTGGGATTTGAGTACAAATGGTGGAACACACT
GCCCTCCAACATGGAAACTACGGTTCTCAGTGGGATCAACTTTGAATACAAGGGCCTGACAGGCTGGGAG
GTGGCTGGTGACCACATTTATACAGCTGTTGGTGCCTCAGACAACGACTTCATGATTCTCACTCTGGTTG
TACCAGGATTTAGACCTCCACAGTCAGTGGTGGCAGACACAGAGAACAAAGAGGTGGCCAGGATCACATT
TGTCTTCGAGACCATCTGCTCTGTGAACTGTGAGCTCTACTTCATGGTGGGCATGAATTCCAGGACCAAT
ACTCCTGTGGAGACGTGGAAGGGCACCAAAGGCAAGCAGTCCTATACCTACACCATCGAAGAGAATGCTA
CTGTGAGCTTCACATGGGCCTTCCAGAGGACCACCCTTCATGAAACAGGCAGGAAGTATACTAATGATGT
CGCCAAGATCTACTCAATCAATGTCACCAACGTCATGGGGGGCGTGGCCTCCTACTGCCGTCCCTGTGCC
CTAGAAGCCTCTGATTTGGGCTCCTCCTGCACCTCTTGTCCTGCTGGCCATTACATCAACCGGGATTCTG
GGACCTGCCACCTCTGTCCCTCTAACACCATCCTGAAAGCTCACCAGCCTTACGGTGCACAGGCCTGTGT
GCCCTGTGGTCCAGGGACCAAGAATAACAAGATCCACTCTCTCTGCTACAACGACTGCACCTTCTCCCGA
AACACCCCCAGCAGGATTTTCAACTACAACTTCTCAGCTCTGGCAGGCACCGTCTCTCTGGCAGGGGTGC
CGAGCTTCACCTCGAAGGGGCTGAAGTATTTTCATCATTTCACCCTGAGTCTCTGTGGAAACCAGGGGAA
AAAAATGGCTGTGTGCACAGACAATGTCACTGACCTCCGGATCCCTGATGGTGAGGCCGGCTTCTCTAAG
TCCGTCACAGCCTATGTCTGCCAGGTTGTCATCATCCCCTCTGAGGTGATGGGCTACAAGGCCGGGGTGT
CCTCACAGCCCGTCAGCCTTGCTGACCGACTTGTTGGAGTGTCAACAGACATGACCTTGGAAGGAATCGT
CTCCCCAGTTGAACTTTTTCACCCAGAGACCTCGGGAATTCCGGACATAGTCTTCTTTTTTAGATCCAAT
GATGTGACCCAGTCCTGCAGTTCTGGAAGATCAACCACCATCCGCCTCAGATGCAATCCAATGAAAGCTG
CTCCTGGCACCCTGCGGCTACCCAGCATGTGCTCTGATGGGACCTGTGATGGCTGTAACTTCCACTTCCT
GTGGGAGAGTGTGGCCGCTTGTCCACTCTGCTCGGCCTCTGACTACCACACTTTTGTCAGCAGCTGTGTG
GCTGGGATCCAGAAGACTACTTACATGTGGCGAGAACCAAAGCTGTGCTCTGGGGGCATTTCCCTGCCTG
AGCAGAGAGTTACCATCTGCAAAACGATCGATTTCTGGTTAAAAGTGGGCATCTCGGCTGGCACCTGCAC
TGCCATCCTGCTTACTGTCCTGACCTGCTACTTTTGGAAGAAGAATCAAAAATTAGAATACAAATACTCC
AAGTTGGTAATGAACGCTACTCTTAAAGACTGCGACCTGCCGGCAGCAGACAGCTGTGCCATCATGGAAG
GAGAGGACGTGGAGGATGACTTCATCTTTACCAGCAAGAAGTCCCTCTTTGGGAAGATCAAATCCTTTAC
CTCCAAGAGGACTCCTGATGGATTTGACTCGGTGCCGCTGAAGACGTCTTCAGGAGGCCCAGACATGGAC
ATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220873 representing NM_001033304
Red=Cloning site Green=Tags(s)

MKDQSCKPCAEGRYSLGTGIRFDEWDELPHGFASLSANLEVDDSISESTENCTSSKWVPRGDYIASNTDE
CTATLMYAVNLKQSGTVNFEYYYPDSSIIFEFFVQNDQCQPSADDSRWMKTTEKGWEFHSVELNRGNNVL
YWRTTAFSVWSKVSKPVLVRNIAITGVAYTSECFPCKPGTYAAKQGSPFCKLCPANYYSNKGETSCHPCD
ADKYSEKGSSTCKVRPACTDKDYFYTHTACDAQGETQLMYKWAIPKICGEDLEGAVKLPASGVKTRCPPC
NPGFFKTDNSTCEPCPYGSYSNGSDCTHCPAGTEPAVGFEYKWWNTLPSNMETTVLSGINFEYKGLTGWE
VAGDHIYTAVGASDNDFMILTLVVPGFRPPQSVVADTENKEVARITFVFETICSVNCELYFMVGMNSRTN
TPVETWKGTKGKQSYTYTIEENATVSFTWAFQRTTLHETGRKYTNDVAKIYSINVTNVMGGVASYCRPCA
LEASDLGSSCTSCPAGHYINRDSGTCHLCPSNTILKAHQPYGAQACVPCGPGTKNNKIHSLCYNDCTFSR
NTPSRIFNYNFSALAGTVSLAGVPSFTSKGLKYFHHFTLSLCGNQGKKMAVCTDNVTDLRIPDGEAGFSK
SVTAYVCQVVIIPSEVMGYKAGVSSQPVSLADRLVGVSTDMTLEGIVSPVELFHPETSGIPDIVFFFRSN
DVTQSCSSGRSTTIRLRCNPMKAAPGTLRLPSMCSDGTCDGCNFHFLWESVAACPLCSASDYHTFVSSCV
AGIQKTTYMWREPKLCSGGISLPEQRVTICKTIDFWLKVGISAGTCTAILLTVLTCYFWKKNQKLEYKYS
KLVMNATLKDCDLPAADSCAIMEGEDVEDDFIFTSKKSLFGKIKSFTSKRTPDGFDSVPLKTSSGGPDMD
M

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033304
ORF Size 2733 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033304.2
RefSeq Size 3326 bp
RefSeq ORF 2736 bp
Locus ID 229722
UniProt ID A2AFS3
Cytogenetics 3 F3
MW 100.5 kDa
Summary May protect cells from cell death by inducing cytosolic vacuolization and upregulating the autophagy pathway.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033304" in other vectors (4)
SKU Description Size Price
MC222556 5330417C22Rik (untagged) - Mouse RIKEN cDNA 5330417C22 gene (5330417C22Rik), (10ug) 10 ug
$918.00
MG220873 5330417C22Rik (tGFP-tagged) - Mouse RIKEN cDNA 5330417C22 gene (5330417C22Rik), (10ug) 10 ug
$1,034.00
MR220873L3 Lenti ORF clone of 5330417C22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 5330417C22 gene (5330417C22Rik) 10 ug
$1,134.00
MR220873L4 Lenti ORF clone of 5330417C22Rik (mGFP-tagged) - Mouse RIKEN cDNA 5330417C22 gene (5330417C22Rik) 10 ug
$1,134.00

Plasmid Map for MR220873

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220873
Circular map for MR220873

file-alt Citations

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