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Mief1 (NM_178719) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220851
Smcr7l (Myc-DDK-tagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7-like (human) (Smcr7l)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Mief1 or NM_178719"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mief1
Synonyms A230016E22; AI452372; Smcr7l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220851 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGCGCTGGTGAGCGCAAAGGGAAGAAGGATGACAATGGCATTGGTACGGCCATCGATTTTGTGC
TCTCCAATGCCCGGCTGGTGCTGGGTGTGGGTGGAGCAGCTATGTTGGGCATCGCCACACTGGCAGTTAA
GCGGATGTATGACCGGGCAATCAGTGCCCCTACCAGCCCTACCCGCCTAAGCCATTCAGGGAAGAGGAGC
TGGGAGGAGCCAAACTGGATGGGCTCTCCTCGCCTATTGAACAAGGACATGAAGGCAGGCCTGAGCCGGT
CCCTGCAGACCCTTCCCACAGACTCTTCTGCCTTTGACACAGATACATTCTGCCCACCCCGGCCCAAACC
ATTGGCCAGGAGGGGCCAGGTAGACTTGAAGAAGTCACGACTCCGCATGTCCCTGCAGGAGAAACTTCTT
TCTTACTACCGGAACCGGGCAGCCATCCCTGCTGGAGAGCAGGCTCGGGCCAAGCAAGCTGCGGTGGACA
TATGTGCTGAGCTCCGGAGCTTCCTGCGGGCCAAGCTGCCTGATATGCCACTTCGGGACATGTACTTGAG
TGGCAGCCTCTATGATGATCTGCAGGTGGTGACGGCTGACCATATCCAACTCATTGTCCCCCTTGTGCTG
GAGCAGAACCTGTGGTCATGCATACCTGGGGAGGACACCATCATGAATGTCCCTGGTTTCTTCCTGGTTC
GTCGTGAGAACCCAGAGTACTTTCCTCGTGGTAGCAGTTATTGGGACCGATGTGTTGTAGGTGGCTACCT
CTCCCCAAAGACAGTGGCAGACACCTTTGAGAAAGTGGTGGCAGGCTCCATCAACTGGCCAGCCATAGGG
TCCCTCTTAGACTATGTGATCCGACCAGCACCACCCCCAGAGGCCCTGACACTAGAAGTTCAGTATGAGA
AGGACAAACATCTTGTCATTGACTTCCTGCCATCAGTAACCCTTGGTGACACTGTCTTGGTGGCCAGACC
ACACCGGTTAGCTCAGTATGACAACCTGTGGCGGCTGAGCCTTCGTCCTGCTGAGACAGCACGCTTACGG
GCTTTGGACCAGGCGGACTCCGGCTGCCGGTCTCTTTGCCTCAAGATCCTCAAGGCCATATGCAAGTCCA
CTCCAGCTCTGGGCCACCTCACTGCCAGCCAGCTAACCAACGTCATCCTCCACTTGGCCCAGGAGGAGGC
TGACTGGTCTCCAGACATGTTGGCTGACCGCTTTCTACAGGCCCTGAGGGGACTCATCAGCTACTTGGAG
GCTGGGGTCTTGCCCAGTGCCCTGAACCCTAAGGTGAACCTATTTGCAGAGCTCACCCCTCAAGAAATAG
ACGAGTTGGGATATACCCTCTACTGCTCACTGTCTGAGCCGGAGGTGCTGCTGCAGACG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220851 protein sequence
Red=Cloning site Green=Tags(s)

MAGAGERKGKKDDNGIGTAIDFVLSNARLVLGVGGAAMLGIATLAVKRMYDRAISAPTSPTRLSHSGKRS
WEEPNWMGSPRLLNKDMKAGLSRSLQTLPTDSSAFDTDTFCPPRPKPLARRGQVDLKKSRLRMSLQEKLL
SYYRNRAAIPAGEQARAKQAAVDICAELRSFLRAKLPDMPLRDMYLSGSLYDDLQVVTADHIQLIVPLVL
EQNLWSCIPGEDTIMNVPGFFLVRRENPEYFPRGSSYWDRCVVGGYLSPKTVADTFEKVVAGSINWPAIG
SLLDYVIRPAPPPEALTLEVQYEKDKHLVIDFLPSVTLGDTVLVARPHRLAQYDNLWRLSLRPAETARLR
ALDQADSGCRSLCLKILKAICKSTPALGHLTASQLTNVILHLAQEEADWSPDMLADRFLQALRGLISYLE
AGVLPSALNPKVNLFAELTPQEIDELGYTLYCSLSEPEVLLQT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178719
ORF Size 1389 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178719.1, NM_178719.2, NM_178719.3, NM_178719.4, NM_178719.5, NP_848834.2
RefSeq Size 5099 bp
RefSeq ORF 1392 bp
Locus ID 239555
UniProt ID Q8BGV8
Cytogenetics 15 E1
MW 51.2 kDa
Summary Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178719" in other vectors (4)
SKU Description Size Price
MC216374 Smcr7l (untagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7-like (human) (Smcr7l), (10ug) 10 ug
$457.00
MG220851 Smcr7l (tGFP-tagged) - Mouse Smith-Magenis syndrome chromosome region candidate 7-like (human) (Smcr7l), (10ug) 10 ug
$657.00
MR220851L3 Lenti ORF clone of Smcr7l (Myc-DDK-tagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7-like (human) (Smcr7l) 10 ug
$757.00
MR220851L4 Lenti ORF clone of Smcr7l (mGFP-tagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7-like (human) (Smcr7l) 10 ug
$757.00

Plasmid Map for MR220851

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220851
Circular map for MR220851

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