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Ipo4 (NM_024267) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220703
Ipo4 (Myc-DDK-tagged) - Mouse importin 4 (Ipo4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $942.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ipo4
Synonyms 8430408O15Rik; AA409693; Imp4a; RanBP4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220703 representing NM_024267
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCTGCGGGTTTGGAACAGATCCTGAAGGAGCTGCTGCTGCCCGACACGGAGCGCATCCGCCGGG
CTACGGAGCAGCTCCAGACCATCCTTCGGGACCCTGCTGCCTTGCCCGCACTCTTCGATCTGCTGGCAAC
AGCGACCGACTCTCAGATCCGCCAGTTTGCAGCGGTCCTGACCCGCAGAAGGCTGAACAATCGCTGGCGA
CGCCTGGCACCTGAGCAGAGGGAGAGCCTCAAGTCCCTGGTCCTGACAGCACTGCAGAAAGAGACAGTGC
ATTCTGTGAGTGTCAGCTTGGCCCAGCTCTCAGCCACCATTTTTCGAAAGGAAGGCCTACAAGGATGGCC
TCAGTTCATGAACCTTCTTCAGCACAGCACTCATAGTTCCCATAGCCCCGAGAAAGAGGTGGGACTTCTG
CTGCTGAGTGTGGTGGTGTCTTCCCAGCCCGAGGCATTCCATGCCCACCAGCACGAGCTGCTTCAGCTTC
TGAATGAGACGCTCAGTGACGTCAGCTTTCCCGGAGTGCTCTTCTACTCCCTGCGCACTCTGACTGCCAT
CGCCCGCTATGTCAGGCCGGATGATGTGTCTCTTGCACGGATGTTGGTGCCCAAAGTCGTTACGGCACTG
AGGACTCTGATCCCCTTAGATGAGGTAAAGGCCTGTGAGGCCCTGGAGGCCCTGGATGAAATGCTGGAGA
CAGAGTTGCCCATCATCAACCCCCACCTCTCTGAGGTCCTCACGTTTTGCCTGGAGGTGGCTAAGAATGT
GGCCCTTGGCGAACCACTCCGCGTACGTGTTCTCTGCTGCCTCACTTTCTTGGTCAAAGTCAAAAGTAAG
GCCTTACTAAAGAACCGCCTCGTGCCTCCCTTGCTGCACGCCCTTTTCCCCCTCATGGCTGCTGAGCCAC
CCATGGGCCAACTGGATCCTGAAGATCAAGATTCGGATGACGATGACTTGGAGATCGGGCTGATGGGGGA
GACTCCCAAGCACTTTGCTGTACAGGTTGTGGACATGCTGGCACTACATCTTCCCCCTGAGAAGCTCTGT
CCCCATGTGATGCCCATGCTGGAAGAAGCCTTGCGGAGCGAGGACCCATACCAGCGCAAAGCAGGATTCC
TGGTGTTGGCTGTGCTGTCTGATGGTGCAGGCGACCACATCAGGCAGAGACTGCTGTACCCACTGCTACA
GATTGTGTGCAAGGGCCTGGATGACCCCTCACAGATTGTTCGTAATGCTGCTCTGTTCGCCCTGGGCCAG
TTTTCAGAGAACTTACAGCCCCATATCAGCAGCTACTCTGAGGAGGTGATGCCCCTTCTCCTCTCCTACC
TGAAGTCAGTGCCGATGGGAAACACACACCACCTGGCCAAGGCCTGCTATGCTCTGGAGAATTTTGTGGA
GAACCTAGGGCCCAAAGTGCAGCCCTACCTTCCGGAGCTTATGGAATGTATGCTGCAGCCATTGAAGAAC
CCCAGCAAAGCCCGGACCAAGGAGCTAGCTGTGAGCGCCATAGGGGCCATTGCCACAGCGGCCCAGGATT
CCCTGCTGCCCTACTTCCCTACCATCATGGATCTTCTCCGGGAGTTCTTGTTGACAGGCCACGAAGACTT
TCATCTTGTGCAGATCCAGAGCCTGGAGACCCTGGGAGTGCTAGCACGAGCCCTGGGAGAGTCCATGAAG
CCCCTGGCTGAGGAATGCTGTCAGCTTGGGCTAGGGTTATGCATCCATATAGATGACCCTGACGTGAGGC
GCTGCACGTACAGCCTGTTTGCAGCCTTATCAGGGTTAATGGGAGAGGGCTTGGGACCCTACCTGCCTCA
GATTACCACGCTCATGCTGCTGTCGCTGCGCTCCACTGAGGGCATTGTGCCACAGTATGACGGGATCAGC
TCCTTCCTGCTGTTTGATGACGACAGCGAAGCAGAAGAGGAGGAGGAGCTCATGGATGAAGATATGGAAG
AGGAGGGGGATGACTCTGAGATCTCGGGGTATAGTGTGGAGAATGCCTTCTTTGATGAGAAAGAAGACAC
CTGCACCGCCCTGGGGGAGATCTCCATGAACACCTGTGTGGCTTTTCTCCCATTCATGGATGCCACCTTC
GATGAAGTATACAAACTGCTGGAGTGCCCTCATATGAATGTGCGGAAGTCAGCTTACGAAGCGCTGGGAC
AGTTCTGCTGTGCACTGCATAAGGCCTCTCAGAGAAGCTCCTCAGATCCCAGCAGCAGTCCCGTTCTGCA
GACCTCCCTGGCTCGAGTGATGCCAGCTTACATGCAGGCCGTGAAAGTAGAAAGGGAGCGCCCAGTGGTG
ATGGCCGTGCTGGAGTCCCTGACAGGGGTGCTGCGCACCTGCGGCAGCCTTGCACTGCAGCCTCCTGGGC
GGCTCAGTGAGCTCTGCAACGTGCTCAAGGCTGTGCTGCAGAAAAAGACGGCCTGTCAGGACGCCGAGGA
GGATGATGACGAAGATGATGACCAGGCAGAGTATGACGCCATGTTACTAGAACATGCTGGAGAGGCCATT
CCCGTCCTGGCAGCCACAGCTGGAGGACACGCCTTCGCTCCCTTCTTTGCCACGTTCTTGCCATTGTTGC
TGTGTAAGACGAAACAGAGCTGTACAGTGGCAGAGAAGTCCTTTGCTGTGGGAACCCTGGCAGAGTCCAT
TCAGGGTCTAGGGACCGCCTCAGCCCAGTTTGTGTCTCGGCTGTTCCCTGTGCTGTTAAACAATGCCCGG
GAAGCAGACCCTGAGGTGCGGAGCAATGCCATCTTTGGGTTGGGCGTACTGGCAGAGCATGGCGGCTGCC
CTGCTCAGGACCACTTCCCTAAGCTACTGGGCCTCCTTTTGCCCCTGCTGGCAAGGGAGCGACATGATCG
AGTCCGTGATAACATCTGCGGGGCTCTTGCCCGTGTACTGATGGCCAGTCCAGTAGGAAAAACGGAGCCC
CAGGTGCTGGCTACCCTGCTACGTGCCCTGCCCCTGAAGGAAGACATGGAGGAGTGGCTCACTATAGGTC
ACCTCTTCAGCTTCCTGCACCAGAACAATCCTGAGCAGGTTGTGGATGTGGCTTCAGAACTCCTGCGCAT
CTGCAGCCTGATCCTGCCGGACAACCGGATCCCTCCAGACACCAAGGCGGCCCTGCTGCTGCTCCTGACG
TTCCTGGCCAAGCAGCACACCGACAGCTTCCACACAGCCCTGGGCTCTCTGCCAAATGATAAAGCTCAGG
AACTTCAGGCCATGATGGGCCTCACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220703 representing NM_024267
Red=Cloning site Green=Tags(s)

MEPAGLEQILKELLLPDTERIRRATEQLQTILRDPAALPALFDLLATATDSQIRQFAAVLTRRRLNNRWR
RLAPEQRESLKSLVLTALQKETVHSVSVSLAQLSATIFRKEGLQGWPQFMNLLQHSTHSSHSPEKEVGLL
LLSVVVSSQPEAFHAHQHELLQLLNETLSDVSFPGVLFYSLRTLTAIARYVRPDDVSLARMLVPKVVTAL
RTLIPLDEVKACEALEALDEMLETELPIINPHLSEVLTFCLEVAKNVALGEPLRVRVLCCLTFLVKVKSK
ALLKNRLVPPLLHALFPLMAAEPPMGQLDPEDQDSDDDDLEIGLMGETPKHFAVQVVDMLALHLPPEKLC
PHVMPMLEEALRSEDPYQRKAGFLVLAVLSDGAGDHIRQRLLYPLLQIVCKGLDDPSQIVRNAALFALGQ
FSENLQPHISSYSEEVMPLLLSYLKSVPMGNTHHLAKACYALENFVENLGPKVQPYLPELMECMLQPLKN
PSKARTKELAVSAIGAIATAAQDSLLPYFPTIMDLLREFLLTGHEDFHLVQIQSLETLGVLARALGESMK
PLAEECCQLGLGLCIHIDDPDVRRCTYSLFAALSGLMGEGLGPYLPQITTLMLLSLRSTEGIVPQYDGIS
SFLLFDDDSEAEEEEELMDEDMEEEGDDSEISGYSVENAFFDEKEDTCTALGEISMNTCVAFLPFMDATF
DEVYKLLECPHMNVRKSAYEALGQFCCALHKASQRSSSDPSSSPVLQTSLARVMPAYMQAVKVERERPVV
MAVLESLTGVLRTCGSLALQPPGRLSELCNVLKAVLQKKTACQDAEEDDDEDDDQAEYDAMLLEHAGEAI
PVLAATAGGHAFAPFFATFLPLLLCKTKQSCTVAEKSFAVGTLAESIQGLGTASAQFVSRLFPVLLNNAR
EADPEVRSNAIFGLGVLAEHGGCPAQDHFPKLLGLLLPLLARERHDRVRDNICGALARVLMASPVGKTEP
QVLATLLRALPLKEDMEEWLTIGHLFSFLHQNNPEQVVDVASELLRICSLILPDNRIPPDTKAALLLLLT
FLAKQHTDSFHTALGSLPNDKAQELQAMMGLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024267
ORF Size 3246 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024267.7
RefSeq Size 3611 bp
RefSeq ORF 3249 bp
Locus ID 75751
UniProt ID Q8VI75
Cytogenetics 14 28.19 cM
MW 119.3 kDa
Summary Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. Acts as chaperone for exposed basic domains.UniProtKB/Swiss-Prot Function
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"NM_024267" in other vectors (4)
SKU Description Size Price
MC223483 Ipo4 (untagged) - Mouse importin 4 (Ipo4), (10ug) 10 ug
$1,037.00
MG220703 Ipo4 (tGFP-tagged) - Mouse importin 4 (Ipo4), (10ug) 10 ug
$1,142.00
MR220703L3 Lenti ORF clone of Ipo4 (Myc-DDK-tagged) - Mouse importin 4 (Ipo4) 10 ug
$1,242.00
MR220703L4 Lenti ORF clone of Ipo4 (mGFP-tagged) - Mouse importin 4 (Ipo4) 10 ug
$1,242.00

Plasmid Map for MR220703

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220703
Circular map for MR220703

file-alt Citations

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