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Plpp4 (NM_001080963) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220596
Ppapdc1a (Myc-DDK-tagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1A (Ppapdc1a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Plpp4 or NM_001080963"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Plpp4
Synonyms C030048B12Rik; Gm1090; Ppapdc1a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220596 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGAGCTGGCCATCGAGATCGGGGTGCGAGCCCTTCTCTTCGGGGTCTTTGTTTTTACAGAATTTT
TGGATCCATTCCAGAGAGTCATCCAGCCGGAAGAGATCTGGCTCTATAAAAATCCTTTGGTGCAGTCAGA
CAACATACCTACCCGCCTCATGTTTGCAATTTCTTTCCTCACACCCTTGGCTGTTATTTGCGTGGTGAAA
ATTATCCGGCGGACAGACAAGACTGAAATTAAGGAAGCCTTCTTAGCAGTGTCCTTGGCTCTTGCTTTGA
ATGGAGTCTGCACAAACACTATTAAACTAATAGTGGGAAGACCTCGCCCCGATTTCTTTTACCGCTGCTT
TCCAGATGGGGTGATGAATTCGGAGATGCGCTGCACAGGTGACCCTGACCTGGTGTCGGAGGGCCGCAAA
AGCTTCCCCAGCATCCATTCCTCCTTTGCCTTCTCGGGCCTCGGTTTCACAACATTCTACCTGGCTGGCA
AGCTGCACTGCTTCACCGAGAGTGGGCGGGGGAAGAGCTGGAGGCTCTGTGCTGCCATCTTGCCCTTGTA
CTGTGCCATGATGATCGCCCTGTCCCGCATGTGTGACTACAAGCATCATTGGCAAGATTCTTTTGTTGGA
GGAGTGATTGGCCTCATTTTTGCCTATATTTGCTACAGACAACATTATCCTCCTCTGGCCAACACAGCAT
GCCACAAACCGTATGTCAGCCTTCGAGTGCCCACTTCACTGAAGAAGGAAGAGAGGCCTACAGCCGACAG
TGCGCCCAGCTTGCCCCTGGAGGGGATCACCGAGGGCCCCGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220596 protein sequence
Red=Cloning site Green=Tags(s)

MRELAIEIGVRALLFGVFVFTEFLDPFQRVIQPEEIWLYKNPLVQSDNIPTRLMFAISFLTPLAVICVVK
IIRRTDKTEIKEAFLAVSLALALNGVCTNTIKLIVGRPRPDFFYRCFPDGVMNSEMRCTGDPDLVSEGRK
SFPSIHSSFAFSGLGFTTFYLAGKLHCFTESGRGKSWRLCAAILPLYCAMMIALSRMCDYKHHWQDSFVG
GVIGLIFAYICYRQHYPPLANTACHKPYVSLRVPTSLKKEERPTADSAPSLPLEGITEGPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001080963
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080963.1, NP_001074432.1
RefSeq Size 1088 bp
RefSeq ORF 816 bp
Locus ID 381925
UniProt ID Q0VBU9
Cytogenetics 7 F3
MW 30.4 kDa
Summary Displays magnesium-independent phosphatidate phosphatase activity in vitro. Catalyzes the conversion of phosphatidic acid to diacylglycerol (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001080963" in other vectors (4)
SKU Description Size Price
MC214738 Ppapdc1a (untagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1A (Ppapdc1a), (10ug) 10 ug
$330.00
MG220596 Ppapdc1a (tGFP-tagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1A (Ppapdc1a), (10ug) 10 ug
$500.00
MR220596L3 Lenti ORF clone of Ppapdc1a (Myc-DDK-tagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1A (Ppapdc1a) 10 ug
$600.00
MR220596L4 Lenti ORF clone of Ppapdc1a (mGFP-tagged) - Mouse phosphatidic acid phosphatase type 2 domain containing 1A (Ppapdc1a) 10 ug
$600.00

Plasmid Map for MR220596

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR220596
Circular map for MR220596

file-alt Citations

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