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Garnl3 (NM_178888) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220535
Garnl3 (Myc-DDK-tagged) - Mouse GTPase activating RANGAP domain-like 3 (Garnl3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $910.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Garnl3
Synonyms AW120551
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220535 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAAGCTCTGTCTCAGAAGACCTAGGCTGTAGACGTGGGGATTTCAGTAGGAAGCATTATGGATCTG
TGGAGCTGCTCATCTCTAGTGATGCCGATGGAGCCATCCAAAGGGCAGGAAGATTCCGAGTGGAGAATGG
CTCTACAGATGAGAGTGCAGCTGCTCTGCCAGGCACTTGGCGAAGGACAGACGTGCACTTAGAAAACCCG
GAGTACCACACCCGGTGGTATTTCAAGTACTTTTTAGGACAAGTCCATCAGAATTACATTGGAAATGATG
CAGAGAAGAGCCCCTTCTTCTTGTCTGTCACCCTTTCTGACCAGAATAATCAGCGTGTCCCTCAGTATCG
TGCAATTCTTTGGCGGAAAACAGGTACGCAAAAAATATGTCTCCCCTACAGTCCCACAAAAACGCTGTCT
GTGAAGTCCATCTTAAGTGCCATGAACCTGGACAAGTTTGAGAAAGGACCTAGGGAAATTTTTCATCCCG
AGATACAAAAGGACTTGCTGGTTCTGGAAGAGCAGGAGGGCTCTGTGAATTTCAAATTTGGGGTTCTTTT
TGCCAAAGATGGGCAGCTCACAGATGACGAGATGTTCAGCAATGAAATTGGAAGTGAAGCTTTTCAAAAA
TTTTTAAATCTCCTCGGTGACACCATTACTCTAAAGGGCTGGACAGGCTACCGTGGCGGTCTGGACACCA
AAAATAACACCACAGGGATCAACTCAGTCTATACTGTGTACCAAGGGCATGAGGTCATGTTTCACGTTTC
CACCATGTTGCCATATTCCAAAGAGAACAGACAGCAGGTGGAAAGGAAGCGCCACATTGGAAACGATATT
GTCACCATCGTGTTCCAGGAAGGAGAGGAGTCCTCTCCCGCCTTTAAGCCTTCCATGATCCGCTCCCATT
TTACACATATTTTTGCCTTAGTGAGGTATGACCAGCAAAATGACAATTACAGGCTGAAAATATTTTCAGA
AGAAAGTGTGCCACTTTTTGGCCCACCCTTGCCATCCCCTCCGGTGTTTACTGACCACCAGGAATTCAGG
GACTTTTTGCTTGTGAAATTAATTAACGGTGAAAAAGCCACTTTGGAAACCCCAACTTTTGCCCAGAAAC
GCCGGCGTACTCTGGACATGTTGATCCGCTCCTTATACCAGGATTTGATGCCGGATTTGCATAAGAATAT
GCTCAATCGGCGATCTTTTAGTGATGTTTTACCGGAGTCACCCAAGTCAGCACGGAAAAAAGAGGAGGCC
CGGCAGGCCGAGTTTGTGAGAATAGGGCAGGCCCTGAAGCTCAAGTCCATTGTGAGAGGGGACGCCCCAT
CAAGCTTGGCAGCATCAGGGATGTGTAAAAAAGAGCCTTGGGAGCCCCAGTGTTTCTGCTGTAATTTCCC
TCATGAAGCCGTGTGTGCAGATCCCTGGGGCCAGGCCTTGCTGGTTTCCACGGATGCTGGCGTCTTGCTA
GTGGACGATGACCTCCCATCCGTGCCCGTGTTTGACAGAACTCTACCTGTGAAGCAGATCCACGTGCTGG
AGACCCTGGACCTTCTGGTTCTCAGAGCAGACAAAGGGAAAGACGCCCGCCTCTTTGTCTTCCGGCTCAG
TGCTGTGCAGAAAGGCCTTGATGGCAGGCAGACTGGAAGGAGCAGATCTGACTGCCGAGAAAACAAGCTG
GAGAAAACCAAAGGATGCCACCTGTATGCTATTAATACTCACCACAGCAGAGAGCTGAGAATCGTGGTTG
CAATTCGCAATAAACTGCTTCTCATCACAAGGAAACCACACAACAAGCCAAGTGGGGTGCCAGGTGTCTC
ACTCCTGTCTCCCCTGTCGGAGTCTCCTGTTGAGGAATTCCAGTACATCAGGGAAATCTGCCTGTGTGAC
TCCCCAGCAGTGATGGCCTTAGTGGATGGACCAACGGAAGAGAGCGACAACCTCATCTGTGTGGCATATC
GCCATCAGTTCGACGTGGTAAACGAGAGCACAGGAGAAGCCTTTAGGCTGCACCATGTGGAGGCCAACAA
GGTTAATTTTGTTGCAGCTATTGATGTTTATGAAGATGGAGAAGCTGGCCTGCTCCTGTGCTACAACTAT
AGTTGCATCTATAAAAAGGTTTGCCCCTTTAACGGTGGCTCTTTTTTGCTTCAACCCTCGGCATCGGATT
TCCAGTTCTGTTGGAACCAGGCTCCATATGCAATTGTCTGCGCCTTCCCATACCTCCTGGCCTTTACTAC
CGACTCCATGGAGATCCGCCTGGTGGTGAATGGGAACCTGGTCCACACGGCAGTTGTGCCACAGCTGCAG
CTCGTGGCCTCCAGGTCAGATATATACTTCACAGCAGCCACGACTGTGCATGAAGGCTCATCTGGAGGCA
GCTCAAAGGGAGCCAGTGCCCACACGTCTCCTCAGACACCACCAGCCCGGGACACTCCACTGTTTCCTTC
TTCCCTGGGGGAAGGTGAAATTCAGTCTAAAAATCTGTACAAGATTCCACTTAGAAACCTTGTGGGCAGA
AGCATCGAGCGACCTCTGAAATCACCCTTAGTCTCCAAGGTCATCACCCCGCCCACCTCCATCGGCCTTG
GAGTCGCTGCCATTCCTGTCACGCATTCCTTGTCCCTCTCCCGCATGGAAATTAAAGAGATTGCAAGCAG
GACCCGCAGGGAACTGCTAGGCCTCTCTGATGATGGTGGAACCAAAACAGAGGGAGCACCAAGGGCCAAA
TCAAAAACCCGGAAGCGGTTGGAAGAAAGCCAAGGAGGCCCCAAGCCAGAGACTGTGAGGTCAGCTAGTA
GTGACAGGATCCCATCAGGAATTTTGGAAAGTCCGGCATCAGAAGCCAATCCCGAGGGCCACAACCACTG
GGCAAGTTCTGAGCAGGACGCTGGGGTGGATAAAGAGGGCAGCCCGGGCTCAGGCAGCAGCCCCTTCCAG
CTCATGGCCTCCTCAGAGGAAGACATCATTGACTTGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220535 protein sequence
Red=Cloning site Green=Tags(s)

MKSSVSEDLGCRRGDFSRKHYGSVELLISSDADGAIQRAGRFRVENGSTDESAAALPGTWRRTDVHLENP
EYHTRWYFKYFLGQVHQNYIGNDAEKSPFFLSVTLSDQNNQRVPQYRAILWRKTGTQKICLPYSPTKTLS
VKSILSAMNLDKFEKGPREIFHPEIQKDLLVLEEQEGSVNFKFGVLFAKDGQLTDDEMFSNEIGSEAFQK
FLNLLGDTITLKGWTGYRGGLDTKNNTTGINSVYTVYQGHEVMFHVSTMLPYSKENRQQVERKRHIGNDI
VTIVFQEGEESSPAFKPSMIRSHFTHIFALVRYDQQNDNYRLKIFSEESVPLFGPPLPSPPVFTDHQEFR
DFLLVKLINGEKATLETPTFAQKRRRTLDMLIRSLYQDLMPDLHKNMLNRRSFSDVLPESPKSARKKEEA
RQAEFVRIGQALKLKSIVRGDAPSSLAASGMCKKEPWEPQCFCCNFPHEAVCADPWGQALLVSTDAGVLL
VDDDLPSVPVFDRTLPVKQIHVLETLDLLVLRADKGKDARLFVFRLSAVQKGLDGRQTGRSRSDCRENKL
EKTKGCHLYAINTHHSRELRIVVAIRNKLLLITRKPHNKPSGVPGVSLLSPLSESPVEEFQYIREICLCD
SPAVMALVDGPTEESDNLICVAYRHQFDVVNESTGEAFRLHHVEANKVNFVAAIDVYEDGEAGLLLCYNY
SCIYKKVCPFNGGSFLLQPSASDFQFCWNQAPYAIVCAFPYLLAFTTDSMEIRLVVNGNLVHTAVVPQLQ
LVASRSDIYFTAATTVHEGSSGGSSKGASAHTSPQTPPARDTPLFPSSLGEGEIQSKNLYKIPLRNLVGR
SIERPLKSPLVSKVITPPTSIGLGVAAIPVTHSLSLSRMEIKEIASRTRRELLGLSDDGGTKTEGAPRAK
SKTRKRLEESQGGPKPETVRSASSDRIPSGILESPASEANPEGHNHWASSEQDAGVDKEGSPGSGSSPFQ
LMASSEEDIIDLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178888
ORF Size 2979 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178888.2, NM_178888.3, NM_178888.4, NP_849219.2
RefSeq Size 3387 bp
RefSeq ORF 2982 bp
Locus ID 99326
UniProt ID Q3V0G7
Cytogenetics 2 B
MW 110.5 kDa
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Documents
Resources
"NM_178888" in other vectors (4)
SKU Description Size Price
MC223005 Garnl3 (untagged) - Mouse GTPase activating RANGAP domain-like 3 (Garnl3), (10ug) 10 ug
$1,001.00
MG220535 Garnl3 (tGFP-tagged) - Mouse GTPase activating RANGAP domain-like 3 (Garnl3), (10ug) 10 ug
$1,110.00
MR220535L3 Lenti ORF clone of Garnl3 (Myc-DDK-tagged) - Mouse GTPase activating RANGAP domain-like 3 (Garnl3) 10 ug
$1,210.00
MR220535L4 Lenti ORF clone of Garnl3 (mGFP-tagged) - Mouse GTPase activating RANGAP domain-like 3 (Garnl3) 10 ug
$1,210.00

Plasmid Map for MR220535

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220535
Circular map for MR220535

file-alt Citations

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