OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Thsd1 (NM_019576) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR220511
Thsd1 (Myc-DDK-tagged) - Mouse thrombospondin, type I, domain 1 (Thsd1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $780.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Thsd1 or NM_019576"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Thsd1
Synonyms 4833423O18Rik; Tmtsp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220511 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACCCATGTTGAAAGACTTTTCAAATCTCTTGCTGGTGGTGCTCTGTGACTATGTCCTCGGAGAAG
CCGAATACCTCCTCCTCCAAGAGCCAGTCCATGTGGCACTGAGCGACAGAACGGTGTCAGTGGGTTTCCA
CTACCTCAGTGACGTCAACGGGACACTGAGGAATGTGTCTGTCATGCTGTGGGAGGCCAACACCAATCGG
ACTCTTACCACTAAGTACCTCCTGACCAACCAGGCCCAAGGAACACTCCAGTTTGAATGTTTCTACTTCA
AAGAGGCTGGTGACTACTGGTTTGTAATGATCCCGGAAGTGACAGACAATGGCACGCAAGTTCCACTCTG
GGAGAAAAGTGCCTTTCTGAAGGTAGAATGGCCTGTCTTTCACATTGATTTAAATAGGACAGCCAAGGCA
GCAGAAGGCACCTTTCAAGTGGGTGTTTTTACCACCCAACCGCTCTGCCTGTTTCCCGTGGACAAGCCAG
ACATGCTGGTGGATGTTATTTTCACTGACCGTCTTCCGGAGGCAAGAGCAAGTTTGGGACAGCCGCTGGA
GATCAGAGCCAGCAAAAGGACAAAACTCACTCAAGGTCAGTGGGTCGAGTTTGGCTGTGCACCGGTAGGG
GTGGAAGCCTACGTTACAGTCATGCTGAGGCTGTTGGGTCAAGACTCAGTCATTGCTTCTACGGGACCTA
TTGACCTGGCTCAAAAATTCGGATACAAATTGATGATGGCACCGGAAGTCACGTGTGAGTCTGTGCTGGA
GGTGATGGTACTGCCACCTCCTTGTGTCTTCGTCCAAGGAGTCCTGGCTGTTTACAAAGAAGCCCCCAAA
CGCCCGGAGGAGAGGACTTTCCAGGTGGCTGAAAACAGACTGCCCCTGGGAGAGAGGAGAACGGTGTTCA
ACTGCACTTTATTTGATGTAGGGAAGAACAAATACTGTTTTAACTTTGGAATTGTGAAGAAAGGCCATTT
TTCTGCAAAGGAATGCATGCTAATTCAGAGAAATATAGAAACTTGGGGACCATGGCAGCCGTGGAGCCCG
TGTAGCACCACGTGCGGGGATGCTGTCCGAGAGCGTCGCCGCCTGTGTGTCACTTCTTTCCCCTCCAGAC
CCAGCTGCTCTGGAATGTCCTCAGAGACCTCTCCATGCTCCCTGGAGGAGTGTGCTGTTTTCCGGCCACC
AGGCCCATCGCCTGTTTCACCCCAGGACCCTGTGAAGTCCAACAACGTGGTGACCGTCACAGGGATCTCC
CTGTGCCTGTTCATCATCTTTGCCACGGTGCTCATCACTCTCTGGAGGAGGTTTGGCCGAGCCCCCAAAT
GCAGCACGCCCGTTCGCCACAACTCCATCCATTCCCCTGGCTTCCGGAAGAACTCCGATGAAGAGAACAT
CTGCGAGCTGAGTGAGCCTCGCGGAAGCTTCTCGGATGCCGGTGACGGACCTAGGGGAAGCCCAGGGGAC
ACGGGCATCCCATTGACTTACAGGTGCAGTGCATCAGCGCCTCCTGAGGATGAGGCCTCGGGCAGTGAGA
GCTTCCAGTCCAACGCTCAGAAGATCATCCCGCCCTTGTTTAGCTACCGCCTTGCCCAGCAGCAGCTGAA
GGAGATGAAGAAGAAAGGGCTGACCGAGACCACCAAAGTGTACCACGTATCTCAAAGCCCCCTGACAGAC
ACTGTAGTGGATGCCACGGCCAGCCCTCCCTTAGACCTGGAATGCCCCGAAGAGGCTGCAGCAAGCAAGT
TCCGAATCAAATCTCCATTTCTGGACCAGCCTGGGGCAGGTACCGGGGAAAGGCCTCCCTCCAGGCTGGA
TGGCATCGTGCCTCCTCCTGGCTGTGCGGTCAGTCCCAGCCAGACCCTGATCCGAAAGTCACAGATAAGG
TCGACCGGTGGCAGAGATGGCTCATCGGAGAGGTGCCACTCCAGAAGTTCCCTCTTCAGGAGGACTGCTA
GTTTTCATGAAACCAAGCAGTCTCGCCCTTTCCGGGAGAGGAGTTTGTCAGCCCTGACTCCCCGCCAGGT
CCCCGCCTACAGTTCCAGGATGCGGACCTGGGACCAGATGGAGGATAGATGTCGGCCTCCCAGTCGAAGT
ACCCACCTGCTTCCAGAGAGACCAGAGCACTTCCAAGGGGCAGGTCGGACCAGCAGTCCTTTGGGTCCAC
TCTCCAAATCCTACACTGTGGGGCATCCCAGGAGGAAACCGGACCCAGGGGATCGTCAGGCCGGATTGGT
GGCAGGAGCTGAGAAAATGGAGCCTCACCGAGCTCACAGGGGACCGTCCCCCAGTCACAGGAGTGCCTCA
AGGAAGCAGTCTTCCCCCATTTTCCTCAAAGATAGCTACCAGAAAGTCAGTCAGCTTAGCCCTTCTCACT
TCAGAAAAGATAAATGCCAGAGCTTCCCCATCCACCCCGAGTTCGCCTTCTATGACAATACCTCTTTCCG
CCTCACCGAGGCTGAGCAGAGAATGCTGGACCTCCCAGGATACTTCGGCTCCAACGAAGAGGACGAAACC
ACAAGTACACTCAGTGTGGAGAAGTTAGTGATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220511 protein sequence
Red=Cloning site Green=Tags(s)

MKPMLKDFSNLLLVVLCDYVLGEAEYLLLQEPVHVALSDRTVSVGFHYLSDVNGTLRNVSVMLWEANTNR
TLTTKYLLTNQAQGTLQFECFYFKEAGDYWFVMIPEVTDNGTQVPLWEKSAFLKVEWPVFHIDLNRTAKA
AEGTFQVGVFTTQPLCLFPVDKPDMLVDVIFTDRLPEARASLGQPLEIRASKRTKLTQGQWVEFGCAPVG
VEAYVTVMLRLLGQDSVIASTGPIDLAQKFGYKLMMAPEVTCESVLEVMVLPPPCVFVQGVLAVYKEAPK
RPEERTFQVAENRLPLGERRTVFNCTLFDVGKNKYCFNFGIVKKGHFSAKECMLIQRNIETWGPWQPWSP
CSTTCGDAVRERRRLCVTSFPSRPSCSGMSSETSPCSLEECAVFRPPGPSPVSPQDPVKSNNVVTVTGIS
LCLFIIFATVLITLWRRFGRAPKCSTPVRHNSIHSPGFRKNSDEENICELSEPRGSFSDAGDGPRGSPGD
TGIPLTYRCSASAPPEDEASGSESFQSNAQKIIPPLFSYRLAQQQLKEMKKKGLTETTKVYHVSQSPLTD
TVVDATASPPLDLECPEEAAASKFRIKSPFLDQPGAGTGERPPSRLDGIVPPPGCAVSPSQTLIRKSQIR
STGGRDGSSERCHSRSSLFRRTASFHETKQSRPFRERSLSALTPRQVPAYSSRMRTWDQMEDRCRPPSRS
THLLPERPEHFQGAGRTSSPLGPLSKSYTVGHPRRKPDPGDRQAGLVAGAEKMEPHRAHRGPSPSHRSAS
RKQSSPIFLKDSYQKVSQLSPSHFRKDKCQSFPIHPEFAFYDNTSFRLTEAEQRMLDLPGYFGSNEEDET
TSTLSVEKLVI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019576
ORF Size 2553 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019576.2, NP_062522.1
RefSeq Size 4432 bp
RefSeq ORF 2556 bp
Locus ID 56229
UniProt ID Q9JM61
Cytogenetics 8 A2
MW 94.7 kDa
Summary Is a positive regulator of nascent focal adhesion assembly, involved in the modulation of endothelial cell attachment to the extracellular matrix.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_019576" in other vectors (4)
SKU Description Size Price
MC222056 Thsd1 (untagged) - Mouse thrombospondin, type I, domain 1 (Thsd1), transcript variant 1, (10ug) 10 ug
$858.00
MG220511 Thsd1 (tGFP-tagged) - Mouse thrombospondin type I domain 1 (Thsd1), (10ug) 10 ug
$980.00
MR220511L3 Lenti ORF clone of Thsd1 (Myc-DDK-tagged) - Mouse thrombospondin, type I, domain 1 (Thsd1), transcript variant 1 10 ug
$1,080.00
MR220511L4 Lenti ORF clone of Thsd1 (mGFP-tagged) - Mouse thrombospondin, type I, domain 1 (Thsd1), transcript variant 1 10 ug
$1,080.00

Plasmid Map for MR220511

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220511
Circular map for MR220511

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products