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Ift74 (NM_026319) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220468
Ift74 (Myc-DDK-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $837.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ift74
Synonyms 1700029H06Rik; b2b796Clo; Ccdc2; Cmg1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220468 representing NM_026319
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGTAATCACAAATCTTCAGCCCCTCGCCCTATTTCTCGGGGTGGAATAGGACTAACAGGAAGGC
CTCCTTCTGGAATAAGACCTCCATCTGGCAATGTTCGAGTGGCAACTGCAATGCCACCAACAACAGCAAG
ACCAGGTTCTCGTGGTGGTCCCTTAGGGACTGGTGGAGTTTTGTCATCTCAAATCAAAGTTGCTGATCGT
CCTGTGACCCAACAAGGTTTGAGTGGAATGAAGACTGGCATGAAAGGTCCCCAGAGGCAAATTTTAGACA
AATCTTACTATCTTGGACTTCTTAGGAGCAAAATAAGTGAACTTACAACTGAAATTAATAAGCTTCAGAA
AGAAATAGAAATGTACAACCAAGAAAATTCAGTGTATTTGTCATATGAAAAGAGGGCAGAGACATTAGCT
GTTGAAATTAAAGACTTTCAAGGACAACTAGCAGACTACAACATGTTGGTAGATAAACTTAATACCAATA
CTGAAATGGAAGAAGTAATGAGTGATTACAACATGCTTAAAGCTCAAAATGACAGAGAAACACAAAGTAT
GGATGTCATATTTACTGAAAGACAAGCGAAGGAAAAACAAATCAGAAGTGTAGAAGAAGAAGTTGAACAA
GAAAAGCAAGCAGCAGATGGCATTATCAAAAATATGTCTCCTGAAAAACAAGTCAAGTACATAGAAATGA
AAACCACAAATGAGAAACTGTTGCAGGAATTAGATACACTTCAGCAACAGCTAGATTCTCTGAACATGAA
AAAGGAGAGCCTAGAAACTGAAATAGCACACTCCCAAGTAAAACAAGAGGCTGTATTGCTGTATGAAAAA
CTTTATGAGTTAGAGTCCCATCGAGATCAAATGATTGCAGAAGACAAAAGCATGGGATCGCCAATGGAGG
AAAGGGAGAGATTGCTTAAGCAGGTTAAAGAAGATAATCAGGAAATAGCCAGCATGGAGAGACAGTTAAC
AGATATAAAAGAAAAAATAAACCAATTTAGTGAAGAAATTAGACAACTTGACATGGATTTAGAGGAGCAC
CAAGGTGAAATGAATCAAAAATACAAGGAGCTTAAAAAAAGGGAGGAAAATATGGATGCTTTTATAGAGA
CTTTTGAGGAAACGAAGAATCAGGAACTAGAGCGGAAGGCACAGATAGAAGCCAGCATCATCACACTGTT
AGAGCATTGCAGTCGGAATATAAATCGTATGAAGCAGATATCCTCCATCACCAACCAAGAGCTGAAGATG
ATGCAGGATGACCTCAGCTTTAAATCAACTGAGATGCAGAAGTCACAGACAACAGCTAGGAATTTGACCT
CAGACAGTCAGCGTCTGCAGCTGGATCTACAGAAAATGGAACTTCTGGAAAGTAAGATGACAGAAGAGCA
ACAGTCTCTGAAAAACAAAATTAAGCAAATGACTGCTGACCTGGAGACATACAGTGACCTGGCAGCTTTA
AAATCATCAGCTGAAGAAAAGAAGAAGAAATTGCATCAAGAAAGAACAGTATTGTCAACTCACAGAAATG
CCTTTAAGAAAATAATGGAGAAGCTGACCTCAGATTATGATACCCTTAAAAGGCAACTTCAAGACAATGA
GACACATGCTCAGCTTACAAATTTGGAGAGGAAGTGGCAGCACCTGGAGCAAAATAACTTCGTAATGAAA
GAGTTCATAGCAACCAAGAGTCAAGAGAGTGATTACCAGCCGGTGATTAAAAATGTGATGAAGCAGATTG
CAGAGTATAATAAAACCATCATGGATGCTCTACATAATGCCAGCAGAAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220468 representing NM_026319
Red=Cloning site Green=Tags(s)

MASNHKSSAPRPISRGGIGLTGRPPSGIRPPSGNVRVATAMPPTTARPGSRGGPLGTGGVLSSQIKVADR
PVTQQGLSGMKTGMKGPQRQILDKSYYLGLLRSKISELTTEINKLQKEIEMYNQENSVYLSYEKRAETLA
VEIKDFQGQLADYNMLVDKLNTNTEMEEVMSDYNMLKAQNDRETQSMDVIFTERQAKEKQIRSVEEEVEQ
EKQAADGIIKNMSPEKQVKYIEMKTTNEKLLQELDTLQQQLDSLNMKKESLETEIAHSQVKQEAVLLYEK
LYELESHRDQMIAEDKSMGSPMEERERLLKQVKEDNQEIASMERQLTDIKEKINQFSEEIRQLDMDLEEH
QGEMNQKYKELKKREENMDAFIETFEETKNQELERKAQIEASIITLLEHCSRNINRMKQISSITNQELKM
MQDDLSFKSTEMQKSQTTARNLTSDSQRLQLDLQKMELLESKMTEEQQSLKNKIKQMTADLETYSDLAAL
KSSAEEKKKKLHQERTVLSTHRNAFKKIMEKLTSDYDTLKRQLQDNETHAQLTNLERKWQHLEQNNFVMK
EFIATKSQESDYQPVIKNVMKQIAEYNKTIMDALHNASRS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026319
ORF Size 1800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026319.3, NP_080595.2
RefSeq Size 2157 bp
RefSeq ORF 1803 bp
Locus ID 67694
UniProt ID Q8BKE9
Cytogenetics 4 C5
MW 69.3 kDa
Summary Component of the intraflagellar transport (IFT) complex B: together with IFT81, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds beta-tubulin via its basic region. Required for ciliogenesis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_026319" in other vectors (4)
SKU Description Size Price
MC219528 Ift74 (untagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74), (10ug) 10 ug
$839.00
MG220468 Ift74 (tGFP-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74), (10ug) 10 ug
$1,037.00
MR220468L3 Lenti ORF clone of Ift74 (Myc-DDK-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74) 10 ug
$1,137.00
MR220468L4 Lenti ORF clone of Ift74 (mGFP-tagged) - Mouse intraflagellar transport 74 homolog (Chlamydomonas) (Ift74) 10 ug
$1,137.00

Plasmid Map for MR220468

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220468
Circular map for MR220468

file-alt Citations

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