Gpr82 (NM_175669) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR220418
Gpr82 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 82 (Gpr82)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr82
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220418 representing NM_175669
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTAATAACTCGACATGCATTCAACCATCCGTGATTTCTACCACAGCTTTACCGGTCACTTACATCT
TTTTGTTTATTATTGGTCTCTTTGGAAACTCACTTGCTCAGTGGGTATTTTTAACAAAAATAGGTAAGAA
AACATCAACACATATCTACTTGGCAAATCTTGTGACTGCAAACTTACTTGTGTGCACTGCTATGCCTTTC
ATGGGTATATATTTCTTGAGAGGTTTTTATTGGAAATATCAATCTGTACAATGCAGACTGGTCAATTTTT
TGGGAACTCTTTCTATGCATGTAAGTATGTTTGTCAGCCTCTTAATTTTAAGCTGGATTGCCATAAGCCG
CTATGCTACCTTAATGAAAAAGGAGTCCAAGCAAGAGGCCACCTCATGCTACGAGAGAATGTTTTATGGT
CACGTACTGAAAAGATTTCGCCAGCCCAACTTTGCCAGAACAATGTGTATTTACATATGGGGAGTTGTGC
TTGTCATAATTATTCCAGTTACCCTATACTACTCAGTAGTCGAGGCTACAGAAGAAGGACAGAGCCAGTG
CTACAATCGGCAGATGGAACTGGGAGCCAGACCTTCTCAGATTGCGGGTCTCATTGGAACCACATTTATT
GGATTCTCCTTTTTAGTAGTAGTGACATCATACTATTCTCTTGTCAGCCATCTGCGAAGAGTAAGGACCT
GTACCTCCATTACCGAGAAAGATTTGACCTACAGATCTGTGAAAAGACACCTTTTGATCATCCAGGTCCT
CCTAGTAGTTTGCTTTCTTCCATATAGTATTTTTAAACCCATTTTTTATGTTCTGCACCAGAGAGAAGGA
GACTGTCAGCAGCTGAATTATTTAATAGAAGCAAAAAACATCCTCACTTGTCTTGCATCAGCCAGAAGTA
GTACAGACCCCATTATATTTCTTTTATTAGATAAAACATTCAAGAAGACACTATATGGTCTCCTTACAAA
ATCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR220418 representing NM_175669
Red=Cloning site Green=Tags(s)

MTNNSTCIQPSVISTTALPVTYIFLFIIGLFGNSLAQWVFLTKIGKKTSTHIYLANLVTANLLVCTAMPF
MGIYFLRGFYWKYQSVQCRLVNFLGTLSMHVSMFVSLLILSWIAISRYATLMKKESKQEATSCYERMFYG
HVLKRFRQPNFARTMCIYIWGVVLVIIIPVTLYYSVVEATEEGQSQCYNRQMELGARPSQIAGLIGTTFI
GFSFLVVVTSYYSLVSHLRRVRTCTSITEKDLTYRSVKRHLLIIQVLLVVCFLPYSIFKPIFYVLHQREG
DCQQLNYLIEAKNILTCLASARSSTDPIIFLLLDKTFKKTLYGLLTKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175669
ORF Size 984 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175669.3, NP_783600.1
RefSeq Size 2497 bp
RefSeq ORF 987 bp
Locus ID 319200
UniProt ID Q8BZR0
Cytogenetics X A1.1
MW 38 kDa
Summary Orphan receptor.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175669" in other vectors (4)
SKU Description Size Price
MC214399 Gpr82 (untagged) - Mouse G protein-coupled receptor 82 (Gpr82), (10ug) 10 ug
$450.00
MG220418 Gpr82 (tGFP-tagged) - Mouse G protein-coupled receptor 82 (Gpr82), (10ug) 10 ug
$650.00
MR220418L3 Lenti ORF clone of Gpr82 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 82 (Gpr82) 10 ug
$750.00
MR220418L4 Lenti ORF clone of Gpr82 (mGFP-tagged) - Mouse G protein-coupled receptor 82 (Gpr82) 10 ug
$750.00

Plasmid Map for MR220418

Download sequence for

Cloning scheme for MR220418

Circular map for MR220418

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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