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Spink12 (NM_030061) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220393
Spink12 (Myc-DDK-tagged) - Mouse serine peptidase inhibitor, Kazal type 11 (Spink12)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spink12
Synonyms 9230117E20Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220393 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCAACGCCCCTAACATTTCTGTGTTCTCAGACCTCAAGATTCTCAGCGCCATGAAGCCAGCAGGTG
CCTTTCTGCTTTTGATCAGCCTGGCCTGTTTGTTCCTCTCTGTAGATGCTGTGAGCCAAGGAGGCTTTCA
GGCTTTTTGCAGCAACTATGAGAAGACACTGGCCCCAGATGGAAAATCATGCCCAAAGACCCACAAACCT
GTGTGTGGCACTGATGGAAAAACCTACCAAAACCGCTGTGCATTCTGCCAAACAGCCATGGAAAGAAGTT
TGGGGAAACTTGGTTTCAAACATGAAGGGAAATGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220393 protein sequence
Red=Cloning site Green=Tags(s)

MFNAPNISVFSDLKILSAMKPAGAFLLLISLACLFLSVDAVSQGGFQAFCSNYEKTLAPDGKSCPKTHKP
VCGTDGKTYQNRCAFCQTAMERSLGKLGFKHEGKC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030061
ORF Size 315 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030061.3, NP_084337.1
RefSeq Size 891 bp
RefSeq ORF 318 bp
Locus ID 78242
UniProt ID Q9D256
Cytogenetics 18 B3
MW 11.3 kDa
Summary Inhibits trypsin.UniProtKB/Swiss-Prot Function
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"NM_030061" in other vectors (4)
SKU Description Size Price
MC211863 Spink12 (untagged) - Mouse serine peptidase inhibitor, Kazal type 11 (Spink12), (10ug) 10 ug
$165.00
MG220393 Spink12 (tGFP-tagged) - Mouse serine peptidase inhibitor Kazal type 11 (Spink12), (10ug) 10 ug
$350.00
MR220393L3 Lenti ORF clone of Spink12 (Myc-DDK-tagged) - Mouse serine peptidase inhibitor, Kazal type 11 (Spink12) 10 ug
$450.00
MR220393L4 Lenti ORF clone of Spink12 (mGFP-tagged) - Mouse serine peptidase inhibitor, Kazal type 11 (Spink12) 10 ug
$450.00

Plasmid Map for MR220393

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220393
Circular map for MR220393

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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