St6gal2 (NM_172829) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR220251
St6gal2 (Myc-DDK-tagged) - Mouse beta galactoside alpha 2,6 sialyltransferase 2 (St6gal2)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol St6gal2
Synonyms C230064G14Rik; mKIAA1877; St6galII
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220251 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACCACACTTGAAGCAATGGCGACAACGAATGCTCTTTGGAATATTTGTTTGGGGGCTCCTCTTTT
TGGCAATTTTCATCTACTTCACCAACAGCAATCCTGCGGCACCTATGCCCAGCTCCTTTTCCTTCCTGGA
GAGACGTGGGCTCCTGCCTCTACAGGGCAAGCAGCGGGTCATCATGGGCGCTTTGCAGGAACCCTCTTTG
CCCAGAAGTTTGGATGCAAGCAAAGTGCTGCTGGACAGCCACCCTGAGAACCCTTTCCACCCTTGGCCTG
GGGACCCACAGAAATGGGATCAGGCCCCAAATGGCTTTGACAATGGGGATGAGTTTTTTACATCCCAGGT
TGGGAGGAAATCACAAAGCGCTTTCTATCCCGAGGAAGATAGCTATTTTTTTGTTGCGGATCAGCCTGAG
TTGTACCACCACAGGCAGGGTGCACTGGAGCTGCCATCTCCAGGGGAGACATCATGGCGATCAGGACCTG
TTCAGCCCAAGCAGAAGCTGCTCCACCCAAGGCGAGGCAGCTTGCCTGAGGAAGCCTATGACAGCGACAT
GCTGTCAGCCTCCATGTCGAGAGCCTTCCTGTACCGGCTCTGGAAGGGGGCCGTGTCCTCTAAGATGTTG
AACCCGCGCCTGCAGAAGGCCATGCGTTACTACATGTCCTTCAACAAGCATGGTGTGCGCTTCCGCAGGC
GGGGTCGGCGTGAAGCTACACGTACAGGGCCGGAGCTGCTGTGTGAGATGCGCAGACGTGTGCGTGTGCG
CACGTTGGACGGCAGAGAGGCGCCCTTCTCGGGGCTGGGCTGGCGGCCTCTGGTACCAGGTGTACCTCTG
AGCCAGTTGCACCCGCGCGGTCTGAGCAGCTGCGCAGTTGTCATGTCTGCCGGTGCCATCCTGAACTCCT
CCTTGGGGGAGGAAATCGATTCTCATGATGCAGTTTTGAGATTTAACTCTGCCCCTACACGTGGCTATGA
GAAAGATGTCGGAAATAAAACCACAGTACGCATCATTAATTCTCAGATTCTGGCCAACCCCAGCCATCAC
TTCATTGACAGTGCTTTATATAAAGATGTTATCCTGGTAGCCTGGGATCCTGCTCCTTATTCTGCCAATC
TTAACCTGTGGTATAAGAAGCCAGATTACAACCTTTTCACTCCATATATCCAGCATCGCCGGAAATACCC
GACTCAGCCATTTTACATTCTTCACCCCAAGTTCATATGGCAGCTTTGGGACATTATCCAGGAGAATACA
AGGGAGAAGATACAGCCCAACCCACCATCTTCTGGTTTTATTGGTACGTGTGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR220251 protein sequence
Red=Cloning site Green=Tags(s)

MKPHLKQWRQRMLFGIFVWGLLFLAIFIYFTNSNPAAPMPSSFSFLERRGLLPLQGKQRVIMGALQEPSL
PRSLDASKVLLDSHPENPFHPWPGDPQKWDQAPNGFDNGDEFFTSQVGRKSQSAFYPEEDSYFFVADQPE
LYHHRQGALELPSPGETSWRSGPVQPKQKLLHPRRGSLPEEAYDSDMLSASMSRAFLYRLWKGAVSSKML
NPRLQKAMRYYMSFNKHGVRFRRRGRREATRTGPELLCEMRRRVRVRTLDGREAPFSGLGWRPLVPGVPL
SQLHPRGLSSCAVVMSAGAILNSSLGEEIDSHDAVLRFNSAPTRGYEKDVGNKTTVRIINSQILANPSHH
FIDSALYKDVILVAWDPAPYSANLNLWYKKPDYNLFTPYIQHRRKYPTQPFYILHPKFIWQLWDIIQENT
REKIQPNPPSSGFIGTCV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172829
ORF Size 1314 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172829.2, NP_766417.1
RefSeq Size 2278 bp
RefSeq ORF 1317 bp
Locus ID 240119
UniProt ID Q76K27
Cytogenetics 17 C
MW 50.3 kDa
Summary Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Has alpha-2,6-sialyltransferase activity toward oligosaccharides that have the Gal-beta-1,4-GlcNAc sequence at the non-reducing end of their carbohydrate groups, but it has weak or no activities toward glycoproteins and glycolipids.UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_172829" in other vectors (4)
SKU Description Size Price
MC215882 St6gal2 (untagged) - Mouse beta galactoside alpha 2,6 sialyltransferase 2 (St6gal2), (10ug) 10 ug
$686.00
MG220251 St6gal2 (tGFP-tagged) - Mouse beta galactoside alpha 26 sialyltransferase 2 (St6gal2), (10ug) 10 ug
$886.00
MR220251L3 Lenti ORF clone of St6gal2 (Myc-DDK-tagged) - Mouse beta galactoside alpha 2,6 sialyltransferase 2 (St6gal2) 10 ug
$986.00
MR220251L4 Lenti ORF clone of St6gal2 (mGFP-tagged) - Mouse beta galactoside alpha 2,6 sialyltransferase 2 (St6gal2) 10 ug
$986.00

Plasmid Map for MR220251

Download sequence for

Cloning scheme for MR220251

Circular map for MR220251

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products