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Otud6a (NM_001163191) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220220
Otud6a (Myc-DDK-tagged) - Mouse OTU domain containing 6A (Otud6a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Otud6a
Synonyms EG408193; Hshin6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220220 representing NM_001163191
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGACACCGAGCAGGAACTCCAACGGGTGATCCGTCGCCACTATCGCGAAAAAAGGGAGCTACAAG
CACACATCCAGACTTTGAAAGCTTCCGTCCCAAAGAACGACAAGGGCAGACGGAAGCAGATGCTCGCGGA
CATCTCCCGCCTCGAGGCCGAGATGGAGCAGAGGCACAAGCAGGAGCTGGAGAAATTTGGAGAAAATCCT
GACAGCAGTGTGGATTCGGTTACAGCCGACCTCGAAAAGATGAATCTCGAGAATATGCCTCCACGCCCAC
CCAAGGCCCAGAAACGGCGCGACCGAAGAGCTCACCAGGAGAGACGGCACCAGGAGAGGATGCCTGCGGC
CCAGGCCGAACAACTGGCTGCCAACCGTCGTGAGGAGGAGGAAAAGGTCGCTGCCATTCTAGGGGCTAAA
AATTTGGAGATGAAGACCATCCCAGCTGACGGCCACTGCATGTATCGTGCCATCCAGGATCAGCTGGTGT
TCTCCGTGACAATCGAGAGCCTTCGCTACCGCACTGCCTACTACATGCGGAAACATATTGATGACTTCTT
ACCTTTCTTCACCGAGCCCGAGGCCGGCAACTTCTACACCCGAGAGGACTTCCTGAGGTACTGTGATGAC
ATTGTGCACAATGCTTCGTGGGGAGGTCAGCTGGAGCTGAGAGCCCTGTCGCATGTCCTGCAGACCCCCA
TCGAGGTGGTACAGGCCAACTCACCCACCATTGTCATCGGGGAGGAGTACACCAGGAAGCCGGTCACGCT
GGTCTATCTGCACTACGCCTGCGACTTCGGAGAGCACTACAATTCGGTGAAGCCCATTGAGGTTGCTGGA
GCATTTGGAGGAATGGCACCGCGCCTCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220220 representing NM_001163191
Red=Cloning site Green=Tags(s)

MSDTEQELQRVIRRHYREKRELQAHIQTLKASVPKNDKGRRKQMLADISRLEAEMEQRHKQELEKFGENP
DSSVDSVTADLEKMNLENMPPRPPKAQKRRDRRAHQERRHQERMPAAQAEQLAANRREEEEKVAAILGAK
NLEMKTIPADGHCMYRAIQDQLVFSVTIESLRYRTAYYMRKHIDDFLPFFTEPEAGNFYTREDFLRYCDD
IVHNASWGGQLELRALSHVLQTPIEVVQANSPTIVIGEEYTRKPVTLVYLHYACDFGEHYNSVKPIEVAG
AFGGMAPRLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001163191
ORF Size 870 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163191.2
RefSeq Size 873 bp
RefSeq ORF 873 bp
Locus ID 408193
UniProt ID Q6IE21
Cytogenetics X
MW 34.2 kDa
Summary Deubiquitinating enzyme that hydrolyzes 'Lys-27'-, 'Lys-29'- and 'Lys-33'-linked polyubiquitin chains. Also able to hydrolyze 'Lys-11'-linked ubiquitin chains (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001163191" in other vectors (4)
SKU Description Size Price
MC214924 Otud6a (untagged) - Mouse OTU domain containing 6A (Otud6a), (10ug) 10 ug
$330.00
MG220220 Otud6a (tGFP-tagged) - Mouse OTU domain containing 6A (Otud6a), (10ug) 10 ug
$500.00
MR220220L3 Lenti ORF clone of Otud6a (Myc-DDK-tagged) - Mouse OTU domain containing 6A (Otud6a) 10 ug
$600.00
MR220220L4 Lenti ORF clone of Otud6a (mGFP-tagged) - Mouse OTU domain containing 6A (Otud6a) 10 ug
$600.00

Plasmid Map for MR220220

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220220
Circular map for MR220220

file-alt Citations

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