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Npm3 (NM_008723) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220197
Npm3 (Myc-DDK-tagged) - Mouse nucleoplasmin 3 (Npm3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Npm3 or NM_008723"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Npm3
Synonyms Nub1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220197 representing NM_008723
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGGCGCGGCGGCTGCTTTAGCGTTCTTGAACCAAGAGAGCCGAGCCCGAGCTGGGGGCGTCG
GGGGCCTGAGGGTCCCGGCCCCGGTCACAATGGACAGTTTCTTCTTCGGTTGTGAGCTCTCCGGCCACAC
CCGCTCCTTCACTTTCAAGGTTGAGGAGGAGGATGACACGGAGCACGTGCTGGCGTTGAACATGCTGTGC
CTTACCGAGGGGGCCACGGACGAGTGTAATGTGGTGGAAGTTGTGGCCCGGGATCACGACAATCAGGAGA
TTGCAGTTCCTGTGGCCAACCTCAGGTTGTCTTGCCAGCCCATGCTCAGTGTGGATGATTTCCAACTCCA
ACCACCTGTCACCTTCCGCCTGAAGTCCGGTTCTGGCCCAGTTCGGATCACTGGCCGGCATCAGATCGTT
TGTATAAACAACGACCTTTCTGAAGAAGAAAGTGATGATGAGAGCGAAGAAGATGAAATTAAGCTGTGTG
GCATCCTTCCTGCCAAGAAGCACAGGGGCAGGCCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220197 representing NM_008723
Red=Cloning site Green=Tags(s)

MAAGAAAALAFLNQESRARAGGVGGLRVPAPVTMDSFFFGCELSGHTRSFTFKVEEEDDTEHVLALNMLC
LTEGATDECNVVEVVARDHDNQEIAVPVANLRLSCQPMLSVDDFQLQPPVTFRLKSGSGPVRITGRHQIV
CINNDLSEEESDDESEEDEIKLCGILPAKKHRGRP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008723
ORF Size 525 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008723.2
RefSeq Size 866 bp
RefSeq ORF 528 bp
Locus ID 18150
UniProt ID Q9CPP0
Cytogenetics 19 38.75 cM
MW 19.5 kDa
Summary Plays a role in the regulation of diverse cellular processes such as ribosome biogenesis, chromatin remodeling or protein chaperoning. Modulates the histone chaperone function and the RNA-binding activity of nucleolar phosphoprotein B23/NPM. Efficiently mediates chromatin remodeling when included in a pentamer containing NPM3 and NPM.UniProtKB/Swiss-Prot Function
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"NM_008723" in other vectors (4)
SKU Description Size Price
MC209048 Npm3 (untagged) - Mouse nucleoplasmin 3 (Npm3), (10ug) 10 ug
$330.00
MG220197 Npm3 (tGFP-tagged) - Mouse nucleoplasmin 3 (Npm3), (10ug) 10 ug
$500.00
MR220197L3 Lenti ORF clone of Npm3 (Myc-DDK-tagged) - Mouse nucleoplasmin 3 (Npm3) 10 ug
$600.00
MR220197L4 Lenti ORF clone of Npm3 (mGFP-tagged) - Mouse nucleoplasmin 3 (Npm3) 10 ug
$600.00

Plasmid Map for MR220197

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220197
Circular map for MR220197

file-alt Citations

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