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Spaca5 (NM_001085393) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220186
Spaca5 (Myc-DDK-tagged) - Mouse sperm acrosome associated 5 (Spaca5)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spaca5
Synonyms Gm718; Lyze
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220186 representing NM_001085393
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGTCTGCAGCATTGTGGTAGTGATCTTGGCCGTATTGTTGATTGCCAAATTGGATGCCAAGATTT
ATGAACGCTGTGAGCTGGCAAAGAAGCTGGAGGAGGCTGGCCTCGATGGCTTCAAAGGCTATACTGTTGG
AGACTGGCTGTGTGTGGCACACTATGAGAGTGGCTTTGACACCTCTTTTGTGGACCACAATCCAGATGGC
AGCAGTGAATATGGCATTTTCCAGCTGAACTCTGCCTGGTGGTGTAACAATGGCATCACACCCACTCAGA
ACCTCTGCAACATCGATTGTAATGACCTGCTCAACCGCCATATTCTGGATGATATCATATGTGCCAAGAG
GGTTGCATCCTCACATAAGAGTATGAAGGCCTGGGATTCCTGGACCCAGCACTGTGCCGGTCATGATTTA
TCAGAATGGCTAAAGGGGTGTTCTGTGCGTCTGAAAACTGACTCAAGCTATAATAACTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220186 representing NM_001085393
Red=Cloning site Green=Tags(s)

MKVCSIVVVILAVLLIAKLDAKIYERCELAKKLEEAGLDGFKGYTVGDWLCVAHYESGFDTSFVDHNPDG
SSEYGIFQLNSAWWCNNGITPTQNLCNIDCNDLLNRHILDDIICAKRVASSHKSMKAWDSWTQHCAGHDL
SEWLKGCSVRLKTDSSYNNW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001085393
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001085393.3
RefSeq Size 554 bp
RefSeq ORF 483 bp
Locus ID 278203
UniProt ID A2AE20
Cytogenetics X A1.3
MW 18 kDa
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"NM_001085393" in other vectors (4)
SKU Description Size Price
MC214344 Spaca5 (untagged) - Mouse sperm acrosome associated 5 (Spaca5), (10ug) 10 ug
$165.00
MG220186 Spaca5 (tGFP-tagged) - Mouse sperm acrosome associated 5 (Spaca5), (10ug) 10 ug
$350.00
MR220186L3 Lenti ORF clone of Spaca5 (Myc-DDK-tagged) - Mouse sperm acrosome associated 5 (Spaca5) 10 ug
$450.00
MR220186L4 Lenti ORF clone of Spaca5 (mGFP-tagged) - Mouse sperm acrosome associated 5 (Spaca5) 10 ug
$450.00

Plasmid Map for MR220186

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220186
Circular map for MR220186

file-alt Citations

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