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Eps8l1 (NM_026146) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220159
Eps8l1 (Myc-DDK-tagged) - Mouse EPS8-like 1 (Eps8l1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $656.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eps8l1
Synonyms 2310051G19Rik; 4632407K17Rik; AW060268; DRC3; EPS8R1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220159 representing NM_026146
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCACCACCACAGGGCCTGAAGCTGCACCCAAACCAAGTGCAAAGTCTATCTATGAACAGAGAAAAC
GTTACTCCACCGTGGTTATGGCTGACGTGTCCCAGTATCATGTCAATCACCTGGTCACCTTCTGCTTGGG
GGAAGAAGATGGCGTGCACACTGTGGAAGATGCTTCCAGAAAGCTGGCAGTCATGGACAGCCAGGGGCGT
GTCTGGGCACAGGAGATGCTACTGAGAGTGTCACCTAGTCAAGTCACTCTGCTTGATCCAGTCTCCAAGG
AAGAACTAGAGTCATATCCTCTCGATGCCATCGTACGCTGCGACGCGGTGATGCCCCGAGGACGGAGCCG
TTCATTGCTGCTCCTGGTGTGCCAGGAGCCAGAGCGTGCACAGCCGGATGTTCATTTCTTCCAGGGCCTG
CTCCTCGGAGCGGAGCTGATCCGTGAGGACATCCAAGGGGCTCTGCAGAACTACCGCTCCGGACGTGGAG
AACGCAGGGCAGCAGCGCTCAGGGCGACGCAGGAGGAGCTGCGACGCGGCGCCTCTCCAGCAGCGGAGAC
CCCGCCCTTACAGCGTCGCCCGTCTGTCCGCCTGGTGATCAACACCGTGGAACCATCCGCAGTCCGCGGG
CGACCCCAAGTGGAGTCAATTCCAGAGACTGAGGAAGCAAGGAAACCCGACCAAGCTCGCACGACTAGCA
GTGCTGACCCTACCTCCCCAGACTTGGGCCCAAGAGGACCTGAGCTGGCGGGTCTGCAGGCAGAGCGGGA
TGTGGATATTCTGAACCACGTGTTTGACGACGTGGAGAGTTTTGTATCCAGACTGCAAAAATCAGCAGAG
GCTACCCGGGTTTTGGAGCACCGCGAGAGAGGCCGCAGGACACGACGCCGAGCTGCGGGAGAGGGTCTGC
TGACACTGAGGGCCAAGCCACCCACTGAGGCTGAGTACACCGACGTGCTTCAGAAAATCAAATACGCCTT
CAGTCTGCTGGCCCGGTTGCGAGGCAACATCGCCAACCCCTCCTCTCCAGAGCTGTTGCACTTTCTGTTT
GGACCTCTGCAGATGATTGTGAACACTTCAGGCGGCCCTGAGTTCGCGAAGAGTGTGCGACGACCGCATT
TGACGTTGGAGGCTGTGACTCTGCTCCGCGACAATGTCACTCCTGGTGAAAATGAACTATGGACCTCGCT
GGGAGACTCCTGGACCTGCCCAGGGGTGGAGCTGCCCCCGGAAGAAGGGTCTCCATACAGTCCGGAATTC
TACAATGGCTGGGAGCCTCCGGCCACTGATCCACAGGGCCGCCCCTGGGAGGACCCAGTGGAAAAACAGC
TACAACACGAGAAGCGGCGCAGGCAGCAAAGCGCCCCCCAGGTCGCGGTCAATGGTCAGCAAGATCCAGA
ACTGGAAACTGAGTCCCAGCTAGAAGAGAAAGCCAGAAAATGGGTTCTGTGTAATTATGACTTCCAGGCC
CGAAATGGCAGCGAGCTATCTGTCAAGCACCGAGATGTGTTGGAGGTCCTGGATGACAGGCGCAAATGGT
GGAAGGTTCGGGACCATCAGGGACAAGAGGGCTATGTACCCTATAATATCCTGACACCCCACCCTGGACC
TCAGGTGCACCGCAGCCAAAGCCCCGCAAGACACCTAGAGACTAGCACACCTCCTCCCCCACCTGCCCCA
GCTCCAGCCCCAACTCAGGTGCGGCCCCAGTGGGACAGTTGCGACAGTCTAAACAGTTTGGATCCCAGCG
AGAAGGAGAAATTCTCCCAGATGCTTTGTGTCAATGAGGAGCTGCAGTCGCGCCTGGCGCAGGGCCGTTC
GGGTCCCAGCCGGGTAACCCCGGGGCCCCGAGCCCAAGAGCCCCAGCTCAGCCCGCGCTCTGAGGCCTCG
GTGGTCCGTGCCTGGCTGCAGACCAAGGGCTTTAGCTCCGGGACTGTGGAGGCGCTCGGTGTGCTGACCG
GCGCACAGCTCTTCTCGCTGCAAAAGGAAGAGTTGCGGGCGGTGTGCCCCGAGGAAGGGGCGCGAGTGTA
CAGCCAAGTCACTGTGCAGCGCGCGCTGCTGGAGGACAGAGAAAAAGTATCAGAGCTGGAGGCCGTGATG
GAGAAGCAAAAGAAAAAAGTGGAAGGCGAGACCAAAACAGAAGTCATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220159 representing NM_026146
Red=Cloning site Green=Tags(s)

MSTTTGPEAAPKPSAKSIYEQRKRYSTVVMADVSQYHVNHLVTFCLGEEDGVHTVEDASRKLAVMDSQGR
VWAQEMLLRVSPSQVTLLDPVSKEELESYPLDAIVRCDAVMPRGRSRSLLLLVCQEPERAQPDVHFFQGL
LLGAELIREDIQGALQNYRSGRGERRAAALRATQEELRRGASPAAETPPLQRRPSVRLVINTVEPSAVRG
RPQVESIPETEEARKPDQARTTSSADPTSPDLGPRGPELAGLQAERDVDILNHVFDDVESFVSRLQKSAE
ATRVLEHRERGRRTRRRAAGEGLLTLRAKPPTEAEYTDVLQKIKYAFSLLARLRGNIANPSSPELLHFLF
GPLQMIVNTSGGPEFAKSVRRPHLTLEAVTLLRDNVTPGENELWTSLGDSWTCPGVELPPEEGSPYSPEF
YNGWEPPATDPQGRPWEDPVEKQLQHEKRRRQQSAPQVAVNGQQDPELETESQLEEKARKWVLCNYDFQA
RNGSELSVKHRDVLEVLDDRRKWWKVRDHQGQEGYVPYNILTPHPGPQVHRSQSPARHLETSTPPPPPAP
APAPTQVRPQWDSCDSLNSLDPSEKEKFSQMLCVNEELQSRLAQGRSGPSRVTPGPRAQEPQLSPRSEAS
VVRAWLQTKGFSSGTVEALGVLTGAQLFSLQKEELRAVCPEEGARVYSQVTVQRALLEDREKVSELEAVM
EKQKKKVEGETKTEVI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026146
ORF Size 2148 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026146.4, NP_080422.1
RefSeq Size 3774 bp
RefSeq ORF 2151 bp
Locus ID 67425
UniProt ID Q8R5F8
Cytogenetics 7 A1
MW 80 kDa
Summary Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton (By similarity).UniProtKB/Swiss-Prot Function
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"NM_026146" in other vectors (4)
SKU Description Size Price
MC221013 Eps8l1 (untagged) - Mouse EPS8-like 1 (Eps8l1), (10ug) 10 ug
$722.00
MG220159 Eps8l1 (tGFP-tagged) - Mouse EPS8-like 1 (Eps8l1), (10ug) 10 ug
$856.00
MR220159L3 Lenti ORF clone of Eps8l1 (Myc-DDK-tagged) - Mouse EPS8-like 1 (Eps8l1) 10 ug
$956.00
MR220159L4 Lenti ORF clone of Eps8l1 (mGFP-tagged) - Mouse EPS8-like 1 (Eps8l1) 10 ug
$956.00

Plasmid Map for MR220159

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220159
Circular map for MR220159

file-alt Citations

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