Olfr147 (NM_146869) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR220148
Olfr147 (Myc-DDK-tagged) - Mouse olfactory receptor 147 (Olfr147)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr147
Synonyms M3; MOR164-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220148 representing NM_146869
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAAGCATGCTGGCTGGAAATGGCTCTTCTGTGACAGAATTTGTCCTTGCTGGTTTGACAGATCGTC
CAGAGCTCCAGCTGCCTCTCTTTTACCTGTTTCTAATAATCTACATAATCACAGTGGTGGGAAACTTGGG
CTTGATCATCCTGATTGGCCTCAATCCTCACCTGCACACCCCCATGTACTATTTCCTCTTCAACCTCTCC
TTCATTGATCTCTGTTACTCTTCTGTCTTCAGCCCCAAAATGCTGATTAACTTTGTCTCTGAGAAGAATT
CCATCTCCTATGCGGGGTGCATGACTCAACTGTTTCTCTTTCTCTTTTTTGTCATCTCTGAATGCTACAT
GTTGACCTCAATGGCCTATGATCGCTATGTGGCCATCTGTAATCCACTATTGTATAAGGTCACCATGTCC
CCTCAGATCTGTTCTGTGATATCTTTTGCTGCGTATGGGATGGGATTTGCTGGATCCTCTGCCCACACAG
GCTGTATGCTCAGACTGACCTTCTGCAATGTCAATGTCATCAACCATTACTTATGTGACATTCTTCCTCT
CCTTCAACTTTCCTGCACCAGTACCTATGTCAATGAGGTTGTGGTTCTCATAGTTGTGGGTATTAACATC
ACAGTTCCAAGCTTCACCATCCTCATTTCCTATGTTTTCATCCTTGCCAACATTCTAAACATCAAATCCA
CACAAGGAAGAGCAAAAGCCTTCAGCACCTGTAGCTCTCACATCATGGCAATTTCTCTTTTCTTTGGATC
AGCTGCATTTATGTATCTTAAATATTCTTCTGGATCTATGGAACAAGGAAAGATATCTTCAGTTTTCTAC
ACTAATGTTGGTCCCATGCTCAACCCTCTGATTTACAGTTTGAGGAATAAGGATGTCAAGGTGGCATTAA
GGAAATCATTGATTAAGTTCAGAGAAAAGACAGATTTTAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR220148 representing NM_146869
Red=Cloning site Green=Tags(s)

MISMLAGNGSSVTEFVLAGLTDRPELQLPLFYLFLIIYIITVVGNLGLIILIGLNPHLHTPMYYFLFNLS
FIDLCYSSVFSPKMLINFVSEKNSISYAGCMTQLFLFLFFVISECYMLTSMAYDRYVAICNPLLYKVTMS
PQICSVISFAAYGMGFAGSSAHTGCMLRLTFCNVNVINHYLCDILPLLQLSCTSTYVNEVVVLIVVGINI
TVPSFTILISYVFILANILNIKSTQGRAKAFSTCSSHIMAISLFFGSAAFMYLKYSSGSMEQGKISSVFY
TNVGPMLNPLIYSLRNKDVKVALRKSLIKFREKTDFN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146869
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146869.2, NP_667080.1
RefSeq Size 1175 bp
RefSeq ORF 954 bp
Locus ID 258869
UniProt ID Q60886
Cytogenetics 9 A5.1
MW 35.8 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_146869" in other vectors (4)
SKU Description Size Price
MC214005 Olfr147 (untagged) - Mouse olfactory receptor 147 (Olfr147), (10ug) 10 ug
$330.00
MG220148 Olfr147 (tGFP-tagged) - Mouse olfactory receptor 147 (Olfr147), (10ug) 10 ug
$500.00
MR220148L3 Lenti ORF clone of Olfr147 (Myc-DDK-tagged) - Mouse olfactory receptor 147 (Olfr147) 10 ug
$600.00
MR220148L4 Lenti ORF clone of Olfr147 (mGFP-tagged) - Mouse olfactory receptor 147 (Olfr147) 10 ug
$600.00

Plasmid Map for MR220148

Download sequence for

Cloning scheme for MR220148

Circular map for MR220148

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