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Tas2r114 (NM_207019) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220134
Tas2r114 (Myc-DDK-tagged) - Mouse taste receptor, type 2, member 114 (Tas2r114)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tas2r114
Synonyms mGR14; mt2r46; T2R14; Tas2r14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220134 representing NM_207019
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAGCACAATGGAAGGTGTCCTCCTTTCAGTTTCAACTAGTGAGGCTGTGCTGGGCATTGTAGGGA
ACACATTCATTGCACTTGTAAACTGTATGGACTATAACAGGAACAAGAAGCTCTCTAATATTGGCTTTAT
TCTCACTGGCTTGGCAATTTCCAGAATTTGCCTTGTGTTGATCTTAATCACAGAGGCATACATAAAAATA
TTCTATCCACAGTTGCTGTCTCCTGTCAACATAATTGAGCTCATCAGTTATCTATGGATAATTATCTGTC
AATTGAATGTCTGGTTTGCCACTAGTCTCAGTATTTTTTATTTCCTGAAGATAGCAAATTTTTCCCACTA
CATATTTGTCTGGTTAAAAAGAAGAATTGATTTAGTTTTTTTCTTCCTGATAGGGTGCTTGCTTATCTCA
TGGCTATTTTCTTTCCCAGTTGTTGCGAAGATGGTTAAAGATAATAAAATGCTGTATATAAACACATCTT
GGCAGATCCACATGAAGAAAAGTGAGTTAATCATTAACTATGTTTTCACCAATGGGGGAGTATTTTTATT
TTTTATGATAATGTTAATTGTATGTTTCCTGTTAATCATTTCACTTTGGAGACATCGCAGGCAGATGGAA
TCAAATAAATTAGGATTCAGAGATCTCAACACAGAAGTTCATGTGAGAACAATAAAAGTTTTATTGTCTT
TTATTATCCTTTTTATATTGCATTTCATGGGTATTACCATAAATGTAATTTGTCTGTTAATCCCAGAAAG
CAACTTGTTATTCATGTTTGGTTTGACAACTGCATTCATCTATCCCGGCTGCCACTCACTTATCCTAATT
CTAGCAAACAGTCGGCTGAAGCAGTGCTCTGTAATGATACTGCAACTATTAAAGTGCTGTGAGAATGGTA
AAGAACTCAGAGACACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220134 representing NM_207019
Red=Cloning site Green=Tags(s)

MLSTMEGVLLSVSTSEAVLGIVGNTFIALVNCMDYNRNKKLSNIGFILTGLAISRICLVLILITEAYIKI
FYPQLLSPVNIIELISYLWIIICQLNVWFATSLSIFYFLKIANFSHYIFVWLKRRIDLVFFFLIGCLLIS
WLFSFPVVAKMVKDNKMLYINTSWQIHMKKSELIINYVFTNGGVFLFFMIMLIVCFLLIISLWRHRRQME
SNKLGFRDLNTEVHVRTIKVLLSFIILFILHFMGITINVICLLIPESNLLFMFGLTTAFIYPGCHSLILI
LANSRLKQCSVMILQLLKCCENGKELRDT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207019
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207019.1, NP_996902.1
RefSeq Size 930 bp
RefSeq ORF 930 bp
Locus ID 387346
UniProt ID Q7M722
Cytogenetics 6 F3
MW 35.7 kDa
Summary Putative taste receptor which may play a role in the perception of bitterness.UniProtKB/Swiss-Prot Function
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"NM_207019" in other vectors (4)
SKU Description Size Price
MC214826 Tas2r114 (untagged) - Mouse taste receptor, type 2, member 114 (Tas2r114), (10ug) 10 ug
$330.00
MG220134 Tas2r114 (tGFP-tagged) - Mouse taste receptor type 2 member 114 (Tas2r114), (10ug) 10 ug
$500.00
MR220134L3 Lenti ORF clone of Tas2r114 (Myc-DDK-tagged) - Mouse taste receptor, type 2, member 114 (Tas2r114) 10 ug
$600.00
MR220134L4 Lenti ORF clone of Tas2r114 (mGFP-tagged) - Mouse taste receptor, type 2, member 114 (Tas2r114) 10 ug
$600.00

Plasmid Map for MR220134

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220134
Circular map for MR220134

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