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Fgd4 (NM_139232) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220124
Fgd4 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 4 (Fgd4), transcript variant alpha
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $701.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fgd4
Synonyms 9030023J02Rik; 9330209B17Rik; Fr; Frabp; ZFYVE; ZFYVE6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220124 representing NM_139232
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGTCTAATCCGGCCCCTACTTCTTGTACCTCAAAAGGGAAGCATAGTAAGGTATCGGATCTCA
TCAGCCACTTTGAAGGAGGCAGTGTTTTGTCAAGTTACATTGATTTGCAGAAAGATTCTACTATGAACCT
CAATATTCCTCAAACTCTAGGACAGCCTGGGTTAACCTCCTCACCTCCACGAAAATTTCTGCCCCAGCAC
TCTCCACAGAAGCAGGAAAACGACCCAGATCAGACTCAGGGGCAACATGGTTGTTTGGCCAATGGTGTAG
TGGCTGCACAAAACCAGATGGAATGTGAAGATGAGAAGGAGACTACTCTTAGCCCAGAGATGGCTATTCA
GACTGCTGCCGCCTCTCCTGATACACATGTGCTGAATGGAGAAAGAAATGAAACCATCACAGATTCTGCA
TCATCCATAGCCAATAGTCATGATGAAAATGCTTCTGACAGCAGCTGCAGGACTCCAGGGACGGACTTAG
GGCTTCCCTCAAAAGAAGGGGAGCCAGGGATGGACGCTGAGCTCCAAGAGAGGGAAAATGGGGTGAACAC
CATGGGATTGGATACGTTGGACCAGCACCATGAAGTGAAGGAGACTAATGAACAGAAACTTCACAAAATA
GCCACTGAACTTTTACTTACAGAAAGAGCTTATGTCAGCCGGCTCGACCTCCTAGATCAGGTATTTTATT
GCAAACTATTAGAAGAAGCAAACCGAGGCTCATTTCCTGCAGAGATGGTGAATAAAATCTTTTCTAACAT
TTCATCAATAAATGCCTTCCATAGTAAATTCCTATTACCTGAGCTGGAGAAACGAATGCAAGAATGGGAA
ACTACACCCAGAATTGGAGATATCCTGCAAAAGTTGGCGCCATTCCTTAAGATGTATGGAGAATACGTGA
AGGGATTTGATAATGCAGTGGAACTGGTTAAAACCATGACAGAGCGTGTTCCCCAGTTTAAATCAGTGAC
TGAAGAGATTCAGAAACAGAAGATCTGTGGAAGCTTAACGCTGCAGCATCACATGCTGGAGCCTATTCAG
CGCATTCCTCGCTATGAGATGCTCCTGAAGGACTACCTGAAGAAGTTGTCTCCTGACTCCCCAGACTGGA
ATGATGCAAAAAAGTCACTTGAAATTATATCTACAGCAGCAAGCCATTCTAATAGTGCAATAAGAAAAAT
GGAGAACCTGAAGAAACTTTTAGAAATTTATGAGATGTTGGGAGAAGAAGAGGATATTGTAAATCCCTCA
AATGAACTAATAAAAGAAGGACAAATCCTCAAACTAGCAGCTCGGAACACATCAGCACAAGAGCGCTACC
TCTTCTTATTCAACAACATGTTGCTATATTGTGTGCCCAGATTCAGCTTGGTTGGCTCAAAATTCACAGT
TCGAACCAGAGTTGGCATTGATGGAATGAAAATTGTGGAGACTCACAATGAAGAATATCCACACACTTTC
CAGATATCTGGGAAAGAAAGAACCCTGGAGCTGCAGGCCAGTTCTGAACAAGACAAGGAAGAATGGATCA
AGGCCCTTCAAGAAAGTATTGATGCTTTTCATCAAAGGCATGAAACTTTCAGAAATGCAATCGCAAAGGA
AAATGACATTCCCCTAGAAGTTTCTACTGCTGAGCTGGGAAAACGAGCTCCAAGATGGATACGTGATAAT
GAAGTGACCATGTGTATGAAGTGCAAAGAGTCCTTCAATGCACTGACCAGAAGGCGGCATCACTGCCGGG
CATGTGGACATGTGGTTTGTTGGAAATGTTCTGACTACAAAGCTCAGCTTGAGTATGATGGTGGAAGATT
GAACAAGGTTTGTAAAGACTGTTACCAGATAATAAGTGGATTCACAGACAGTGAAGAAAAGAAAAGAAGG
GGCATTTTAGAGATTGAATCAGCAGAAGTATCAGGAAATAGTGAGGTGTGCAGTTTCCTTCAGTACATGG
AAAAGTCAAAGCCTTGGCAGAAAATCTGGTGTGTGATCCCCAAGCAAGACCCCCTTGTGCTGTACATGTA
TGGCGCTCCTCAGGATGTCAGAGCCCAAGCGACCATACCCCTCCTGGGCTACGTTGTGGATGATATGCCT
AAGAGTGCAGATCTGCCACATAGTTTCAAGCTGACCCAGTCCAAGTCTGTGCATAGCTTTGCTGCAGACA
ATGAGGAACTGAAACAGAAATGGCTGAAAATCATCCTTCTAGCTGTCACAGGTGAGACACCAGATGGCCC
AAGTGAGCATCTAGCCACCTTGAACAATCTCCCTGGACCCAAGAAAAAGTCAGAATGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220124 representing NM_139232
Red=Cloning site Green=Tags(s)

MEESNPAPTSCTSKGKHSKVSDLISHFEGGSVLSSYIDLQKDSTMNLNIPQTLGQPGLTSSPPRKFLPQH
SPQKQENDPDQTQGQHGCLANGVVAAQNQMECEDEKETTLSPEMAIQTAAASPDTHVLNGERNETITDSA
SSIANSHDENASDSSCRTPGTDLGLPSKEGEPGMDAELQERENGVNTMGLDTLDQHHEVKETNEQKLHKI
ATELLLTERAYVSRLDLLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPELEKRMQEWE
TTPRIGDILQKLAPFLKMYGEYVKGFDNAVELVKTMTERVPQFKSVTEEIQKQKICGSLTLQHHMLEPIQ
RIPRYEMLLKDYLKKLSPDSPDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEIYEMLGEEEDIVNPS
NELIKEGQILKLAARNTSAQERYLFLFNNMLLYCVPRFSLVGSKFTVRTRVGIDGMKIVETHNEEYPHTF
QISGKERTLELQASSEQDKEEWIKALQESIDAFHQRHETFRNAIAKENDIPLEVSTAELGKRAPRWIRDN
EVTMCMKCKESFNALTRRRHHCRACGHVVCWKCSDYKAQLEYDGGRLNKVCKDCYQIISGFTDSEEKKRR
GILEIESAEVSGNSEVCSFLQYMEKSKPWQKIWCVIPKQDPLVLYMYGAPQDVRAQATIPLLGYVVDDMP
KSADLPHSFKLTQSKSVHSFAADNEELKQKWLKIILLAVTGETPDGPSEHLATLNNLPGPKKKSEC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_139232
ORF Size 2298 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139232.3, NP_631978.1
RefSeq Size 8344 bp
RefSeq ORF 2301 bp
Locus ID 224014
UniProt ID Q91ZT5
Cytogenetics 16 A2- A3
MW 86.5 kDa
Summary This gene is a member of the FYVE, RhoGEF and PH domain containing (FGD) family. The encoded protein is a Cdc42-specific guanine nucleotide exchange factor (GEF) that plays an essential role in regulating the actin cytoskeleton and cell morphology. Disruption of the gene in mouse causes abnormal nerve development and dysmyelination. Mutations in a similar gene in human can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2014
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"NM_139232" in other vectors (4)
SKU Description Size Price
MC221459 Fgd4 (untagged) - Mouse FYVE, RhoGEF and PH domain containing 4 (Fgd4), transcript variant alpha, (10ug) 10 ug
$772.00
MG220124 Fgd4 (tGFP-tagged) - Mouse FYVE RhoGEF and PH domain containing 4 (Fgd4) transcript variant alpha, (10ug) 10 ug
$901.00
MR220124L3 Lenti ORF clone of Fgd4 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 4 (Fgd4), transcript variant alpha 10 ug
$1,001.00
MR220124L4 Lenti ORF clone of Fgd4 (mGFP-tagged) - Mouse FYVE, RhoGEF and PH domain containing 4 (Fgd4), transcript variant alpha 10 ug
$1,001.00

Plasmid Map for MR220124

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220124
Circular map for MR220124

file-alt Citations

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