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Rbbp9 (NM_015754) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR220115
Rbbp9 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 9 (Rbbp9)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Rbbp9 or NM_015754"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rbbp9
Synonyms Bog
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR220115 representing NM_015754
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCCCCAACAAGGCAGTGATTGTTCCCGGGAACGGAGGCGGGGATGTGGCCACCCACGGCTGGT
ACGGCTGGGTGAAAAAGGGGCTGGAGCAGATTCCTGGTTTCCAGTGTTTGGCTAAAAACATGCCTGACCC
AATTACAGCGCGAGAGAGCATCTGGCTGCCCTTCATGGAGACAGAGCTGCACTGTGACGAGAAGACCATC
ATCATAGGCCACAGTTCCGGGGCCATCGCAGCCATGAGGTATGCAGAGACACATCAGGTATATGCTCTCG
TATTGGTGTCTGCATACACATCAGACTTGGGAGATGAAAATGAGCGTGCAAGTGGGTACTTCAGCCGCCC
CTGGCAGTGGGAGAAGATCAAGGCCAACTGCCCTCACATTGTACAGTTTGGCTCTACTGATGACCCCTTC
CTTCCCTGGAAGGAACAACAAGAAGTAGCAGATAGGCTGGACGCCAAATTGTACAAATTCACTGACCGTG
GTCACTTTCAGAACACAGAGTTCCATGAACTGATTAGTGTGGTGAAGTCTATGCTGAAAGGACCAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR220115 representing NM_015754
Red=Cloning site Green=Tags(s)

MASPNKAVIVPGNGGGDVATHGWYGWVKKGLEQIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTI
IIGHSSGAIAAMRYAETHQVYALVLVSAYTSDLGDENERASGYFSRPWQWEKIKANCPHIVQFGSTDDPF
LPWKEQQEVADRLDAKLYKFTDRGHFQNTEFHELISVVKSMLKGPE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015754
ORF Size 558 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015754.2, NP_056569.2
RefSeq Size 2183 bp
RefSeq ORF 561 bp
Locus ID 26450
UniProt ID O88851
Cytogenetics 2 G1
MW 20.9 kDa
Summary May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta1 through interaction with retinoblastoma and the subsequent displacement of E2F-1.UniProtKB/Swiss-Prot Function
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"NM_015754" in other vectors (4)
SKU Description Size Price
MC209733 Rbbp9 (untagged) - Mouse retinoblastoma binding protein 9 (Rbbp9), (10ug) 10 ug
$330.00
MG220115 Rbbp9 (tGFP-tagged) - Mouse retinoblastoma binding protein 9 (Rbbp9), (10ug) 10 ug
$500.00
MR220115L3 Lenti ORF clone of Rbbp9 (Myc-DDK-tagged) - Mouse retinoblastoma binding protein 9 (Rbbp9) 10 ug
$600.00
MR220115L4 Lenti ORF clone of Rbbp9 (mGFP-tagged) - Mouse retinoblastoma binding protein 9 (Rbbp9) 10 ug
$600.00

Plasmid Map for MR220115

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR220115
Circular map for MR220115

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