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Xrcc3 (NM_028875) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219979
Xrcc3 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Xrcc3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219979 representing NM_028875
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTTGGATCAGCTGGACCTAAACCCCCGAATTACTGCTGCGGTTAAGAGGGGCAGACTGAAGTCAG
TGAAGGAGATTCTGTGCTACTCCGGACCAGACTTGCAGCGGCTCACCGGCCTGCCCAGCCACGATGTGCA
GTGCCTGCTGAGAGCTGCCTCTCTACACCTTCGGGGAAGCCGCGTCCTCTCAGCACTGCATCTGTTCCAG
CAGAAGGAGAGCTTCCCTGAGCAGCATCAACGCCTGAGCCTAGGCTGCCCTGTCCTGGATCAGTTCCTGG
GTGGAGGCCTGCCCCTGGAGGGCATCACTGGCCTGGCTGGCTGCAGCTCAGCAGGAAAGACCCAGCTGGC
GCTACAGCTCTGCCTGGCTGTGCAGTTCCCAAGACAATATGGAGGCCTAGAGGCCGGGGCTGTCTACATC
TGCACAGAAGATGCCTTCCCCAGCAAGCGGCTGTGGCAGCTCATCGCGCAGCAGCGGAGGCTGCGGACAG
ACGCGCCTGAGGAGCTGATCGAGAAGATCAGGTTCAGCAACCACATCTTCATTGAACACGCGGCCGACGT
GGACACCTTGTTGGAGTGTGTGAGTAAGAAAGTCCCCATCCTGCTGTCGAGGGGCATGGCCCGCCTGGTG
GTGGTTGACTCCATTGCAGCCCCATTTCGTTGTGAGTTCCACCTTCAGGCCTCAGCCATCAGGGCGAAGC
TCCTGCTCTCGCTGGGGGCCACACTGCGAAGACTGAGCAGCACCTTCAGGAGCCCAGTGCTGTGCATCAA
CCAGGTGACAGACATGGTGGAGGACCAGCAGTCTGTGTCCAGGTCACTAGGGGCTTCAGAAGAGCGCCTC
TCTCCAGCCCTTGGCATCACCTGGGCTAACCAGCTCCTGATGCGACTGATGGTTGACCGCACCCATGAGG
ACGATGTTACCACAGGCTTGCCCAGAAGCCCAGTTCGGACTCTCCGAGTGCTCTTCGCCCCCCACCTACC
CCTCTCCTCCTGCTGCTACACAGTCAGTGGGGAAGGCATTAGGGGGATGCCAGGAACACAGTCCTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219979 representing NM_028875
Red=Cloning site Green=Tags(s)

MDLDQLDLNPRITAAVKRGRLKSVKEILCYSGPDLQRLTGLPSHDVQCLLRAASLHLRGSRVLSALHLFQ
QKESFPEQHQRLSLGCPVLDQFLGGGLPLEGITGLAGCSSAGKTQLALQLCLAVQFPRQYGGLEAGAVYI
CTEDAFPSKRLWQLIAQQRRLRTDAPEELIEKIRFSNHIFIEHAADVDTLLECVSKKVPILLSRGMARLV
VVDSIAAPFRCEFHLQASAIRAKLLLSLGATLRRLSSTFRSPVLCINQVTDMVEDQQSVSRSLGASEERL
SPALGITWANQLLMRLMVDRTHEDDVTTGLPRSPVRTLRVLFAPHLPLSSCCYTVSGEGIRGMPGTQSY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028875
ORF Size 1047 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028875.3, NP_083151.1
RefSeq Size 2422 bp
RefSeq ORF 1050 bp
Locus ID 74335
UniProt ID Q9CXE6
Cytogenetics 12 F1
MW 38.9 kDa
Summary This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. Allelic variants in the human gene are associated with susceptibility to breast cancer and cutaneous malignant melanoma. provided by RefSeq, Sep 2015
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"NM_028875" in other vectors (4)
SKU Description Size Price
MC211524 Xrcc3 (untagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3), (10ug) 10 ug
$503.00
MG219979 Xrcc3 (tGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3), (10ug) 10 ug
$657.00
MR219979L3 Lenti ORF clone of Xrcc3 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3) 10 ug
$757.00
MR219979L4 Lenti ORF clone of Xrcc3 (mGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 3 (Xrcc3) 10 ug
$757.00

Plasmid Map for MR219979

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219979
Circular map for MR219979

file-alt Citations

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